Abstract
Aging is associated with alterations in the arterial wall that promote vascular disease development and its clinical manifestations, including myocardial infarction, stroke, and arterial dissection. The arterial wall is comprised of three layers, intima, media and adventitia, each with distinct cellular composition and function, which can therefore contribute differently to vascular disease initiation and progression. Hence, studying transcriptomic alterations, either in the entire arterial wall or separately in the three arterial layers, can aid in disentangling the etiopathology of vascular disease and thus pave the way for innovative treatments. This chapter describes protocols for total RNA extraction from complete mouse aorta and separately from intima, media, and adventitia layers for subsequent transcriptomic analysis.
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Acknowledgments
We thank Pilar Gonzalo for optimizing intima collection protocol. Work in Dr. Andrés’ laboratory is supported by the Spanish Ministerio de Ciencia e Innovación (MCIN)/Agencia Estatal de Investigación (AEI)/10.13039/501100011033 (grant PID2019-108489RB-I00) and the Instituto de Salud Carlos III (ISCIII) (AC16/00091, AC17/00067, and CB16/11/00405) with cofunding from the European Regional Development Fund (ERDF/FEDER, “Una manera de hacer Europa”), and the Progeria Research Foundation. R.M.N. is supported by the Ministerio de Educación, Cultura y Deporte (pre-doctoral contract FPU16/05027). M.R.H. is supported by the MCIN (post-doctoral contract IJC2019-040798-I). The CNIC is supported by the MCIN, the ISCIII, and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (grant CEX2020-001041-S funded by MICIN/AEI/10.13039/501100011033).
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Nevado, R.M., Hamczyk, M.R., Andrés, V. (2022). Isolation of Mouse Aortic RNA for Transcriptomics. In: Ramji, D. (eds) Atherosclerosis. Methods in Molecular Biology, vol 2419. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1924-7_38
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DOI: https://doi.org/10.1007/978-1-0716-1924-7_38
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