Abstract
Biosynthesis method has emerged as simple, nontoxic, and environmental friendly and has been developed to produce Gold nanoparticles (AuNPs). In the present protocol, the extracellular synthesis of AuNPs is carried out using a culture supernatant of isolated actinomycetes. Bioreduction of AuNPs is confirmed by a UV-visible spectrophotometer that shows the peak between 520 and 550 nm. The size and distribution of the biosynthesized AuNPs are analyzed by HR-TEM that shows the formation of AuNPs. The crystalline nature and mean size of the AuNPs can be confirmed using XRD. The study shows the rapid and eco-friendly synthesis of AuNPs from actinomycetes.
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References
Hulkoti NI, Taranath T (2014) Biosynthesis of nanoparticles using microbes-a review. Colloids Surf B: Biointerfaces 121:474–483
Ranjitha V, Rai VR (2017) Actinomycetes mediated synthesis of gold nanoparticles from the culture supernatant of Streptomyces griseoruber with special reference to catalytic activity. 3 Biotech 7(5):299
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Vimala, R.T.V. et al. (2022). Biosynthesis and Characterization of Gold Nanoparticles from Actinobacteria. In: Dharumadurai, D. (eds) Methods in Actinobacteriology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1728-1_104
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DOI: https://doi.org/10.1007/978-1-0716-1728-1_104
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1727-4
Online ISBN: 978-1-0716-1728-1
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