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Imaging of the Cytoskeleton Using Live and Fixed Drosophila Tissue Culture Cells

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Cytoskeleton

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2364))

Abstract

In recent years, the convergence of multiple technologies and experimental approaches has led to the expanded use of cultured Drosophila cells as a model system. Their ease of culture and maintenance, susceptibility to RNA interference, and imaging characteristics have led to extensive use in both traditional experimental approaches and high-throughput RNAi screens. Here we describe Drosophila S2 cell culture and preparation for live-cell and fixed-cell fluorescence microscopy and scanning electron microscopy.

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Author information

Authors and Affiliations

  1. Department of Biology, Reed College, Portland, OR, USA

    Derek A. Applewhite

  2. Nikon Imaging Center, University of California San Diego, San Diego County, CA, USA

    Eric R. Griffis

  3. Department of Biology, University of Richmond, Richmond, VA, USA

    Christine A. Lacy & Omar A. Quintero-Carmona

Authors
  1. Derek A. Applewhite
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  2. Christine A. Lacy
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  3. Eric R. Griffis
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  4. Omar A. Quintero-Carmona
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Corresponding author

Correspondence to Omar A. Quintero-Carmona .

Editor information

Editors and Affiliations

  1. Professor Emeritus, Brooklyn College – CUNY, Brooklyn, NY, USA

    Ray H. Gavin

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Applewhite, D.A., Lacy, C.A., Griffis, E.R., Quintero-Carmona, O.A. (2022). Imaging of the Cytoskeleton Using Live and Fixed Drosophila Tissue Culture Cells. In: Gavin, R.H. (eds) Cytoskeleton . Methods in Molecular Biology, vol 2364. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1661-1_8

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  • DOI: https://doi.org/10.1007/978-1-0716-1661-1_8

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1660-4

  • Online ISBN: 978-1-0716-1661-1

  • eBook Packages: Springer Protocols

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