Abstract
A sensitive, rapid, and cost-effective method for quantitative analysis of proteins (e.g., detection, purification, depletion) for a wide variety of purposes is required in a number of areas, such as immunodiagnostics and biotechnology. Double-layer imprinting technique, which is carried out via polymerization of polymer solution with higher monomer concentration, covering and filling the supermacroporous structure of a pre-synthesized cryogel column with a lower monomer concentration, thus improving the surface area and adsorption capacity of final product, is a brand new approach for the application of cryogels in molecular imprinting technology. Within the scope of this chapter, BSA is selected as a model protein for the application of double-layer imprinting protocol. In this chapter, synthesis of double-layer BSA-imprinted and non-imprinted cryogel columns (BSA-DLIP and DLNIP, respectively) are described. In addition, characterization of synthesized columns and BSA depletion studies from aqueous solutions are described in detail, as well as selectivity of BSA-DLIPs for BSA, against competitors.
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Zenger, O., Baydemir Peşint, G. (2021). Synthesis of Double-Layer Imprinted Polymers: BSA Depletion. In: Martín-Esteban, A. (eds) Molecularly Imprinted Polymers. Methods in Molecular Biology, vol 2359. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1629-1_6
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DOI: https://doi.org/10.1007/978-1-0716-1629-1_6
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