Abstract
The recent development of the bright luciferase NanoLuc (Nluc) has greatly improved the sensitivity of bioluminescence imaging, enabling real-time cellular imaging with high spatial resolution. However, the limited color variants of Nluc have restricted its wider application to multicolor imaging of biological phenomena. To address this issue, we developed five new spectral variants of the bright bioluminescent protein with emissions across the visible spectrum. In this chapter, we describe the following two protocols for single-cell bioluminescence imaging: (a) multicolor bioluminescence imaging of subcellular structures and (b) multicolor calcium imaging in single living cells.
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Acknowledgments
This work was supported by a Grant-in-Aid for Scientific Research (A) of MEXT (No. 26251018), the JST-SENTAN program, The Uehara Memorial Foundation, and the Naito Foundation to T.N. K.S. was supported by Grant-in-Aid 18J01772 for the JSPS Research Fellow.
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Suzuki, K., Hossain, M.N., Matsuda, T., Nagai, T. (2021). Multicolor Bioluminescence Imaging of Subcellular Structures and Multicolor Calcium Imaging in Single Living Cells. In: Zamir, E. (eds) Multiplexed Imaging. Methods in Molecular Biology, vol 2350. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1593-5_14
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DOI: https://doi.org/10.1007/978-1-0716-1593-5_14
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