Abstract
Electrochemical biosensors offer a sensitive, specific, and rapid detection platform for in situ real-time monitoring of intracellular and extracellular metabolites. These sensors have been widely used to evaluate the efficacy of preclinical drugs, especially for natural products with antioxidant potency. Ultraviolet (UV) radiation causes oxidative stress in cells and induces cells to release reactive oxygen species. Tocopherol is a fat-soluble vitamin found in vegetable oils as well as in grains, seeds, and nuts, which plays an important protective role as an antioxidant in resisting oxidative stress caused by UV radiation. Here, we describe a protocol using a glass carbon electrode functionalized with nanotube@DNA-Mn3(PO4)2 composite to monitor and quantify the production of superoxide ions in UV-irradiated melanoma cells in the presence or absence of tocopherol. This study demonstrates the advantages and potential application of label-free electrochemical sensors in the measurement of natural antioxidants from plant materials.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Gao L, Teng Y (2016) Exploiting plug-and-play electrochemistry for drug discovery. Future Med Chem 8(5):567–577
Gao L, Wang X, Tang Y, Huang S, Hu CA, Teng Y (2017) FGF19/FGFR4 signaling contributes to the resistance of hepatocellular carcinoma to sorafenib. J Exp Clin Cancer Res 36(1):8. https://doi.org/10.1186/s13046-016-0478-9
Berwick M, Lachiewicz A, Pestak C, Thomas N (2008) Solar UV exposure and mortality from skin tumors. Adv Exp Med Biol 624:117–124
Duthie MS, Kimber I, Dearman RJ, Norval M (2000) Differential effects of UVA1 and UVB radiation on Langerhans cell migration in mice. J Photochem Photobiol B 57(2-3):123–131
Dizdaroglu M, Jaruga P, Birincioglu M, Rodriguez H (2002) Free radical-induced damage to DNA: mechanisms and measurement. Free Radic Biol Med 32(11):1102–1115
Gao LX, Bian C, Wu Y, Nisar MF, Chen S, Li CM et al (2018) Label-free electrochemical sensor to investigate the effect of tocopherol on generation of superoxide ions following UV irradiation. J Biol Eng 12:17. https://doi.org/10.1186/s13036-018-0099-2
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Gao, L., Teng, Y. (2022). Label-Free Electrochemical Biosensors to Evaluate the Antioxidant Effect of Tocopherol in Ultraviolet Radiation. In: Guest, P.C. (eds) Physical Exercise and Natural and Synthetic Products in Health and Disease. Methods in Molecular Biology, vol 2343. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1558-4_16
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1558-4_16
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1557-7
Online ISBN: 978-1-0716-1558-4
eBook Packages: Springer Protocols