Abstract
Although flow cytometry and cell sorting are widely used by immunologists both for basic and translation research, many aspects of these techniques should be optimized to obtain reproducible and meaningful data. In this chapter we provide some protocols and tips on instrument setting, multicolor panel design and T-cell immunophenotyping and proliferation assay.
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Anselmo, A., Colombo, F.S. (2021). Flow Cytometry Instrument Setting as a Crucial Checkpoint for Optimal T-Cell Analysis and Sorting. In: Gigante, M., Ranieri, E. (eds) Cytotoxic T-Cells. Methods in Molecular Biology, vol 2325. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1507-2_1
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DOI: https://doi.org/10.1007/978-1-0716-1507-2_1
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1506-5
Online ISBN: 978-1-0716-1507-2
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