Abstract
The expression of mammalian recombinant proteins in insect cell lines using transient-plasmid-based gene expression enables the production of high-quality protein samples. Here, the procedure for virus-free transient gene expression (TGE) in High Five insect cells is described in detail. The parameters that determine the efficiency and reproducibility of the method are presented in a robust protocol for easy implementation and set-up of the method. The applicability of the TGE method in High Five cells for proteomic, structural, and functional analysis of the expressed proteins is shown.
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Schubert, M., Nimtz, M., Bertoglio, F., Schmelz, S., Lukat, P., van den Heuvel, J. (2021). Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells. In: Owens, R.J. (eds) Structural Proteomics. Methods in Molecular Biology, vol 2305. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1406-8_6
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DOI: https://doi.org/10.1007/978-1-0716-1406-8_6
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1405-1
Online ISBN: 978-1-0716-1406-8
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