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Choosing Fluorescent Probes and Labeling Systems

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Confocal Microscopy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2304))

Abstract

Fluorescence microscopy is advantageous for investigating biological processes and mechanisms in living cells. One of the most important considerations when designing an experiment is the selection of an appropriate fluorescent probe. Equally important is deciding how the probe will be attached to the protein of interest. The advantages and disadvantages of different fluorescent probe types and their respective labeling methods are discussed to provide an overview on selecting appropriate fluorophores and labeling systems for fluorescence-based assays. Protocols are outlined when appropriate.

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Author information

Authors and Affiliations

  1. Section on Biophotonics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA

    Kimberly Jacoby-Morris & George H. Patterson

Authors
  1. Kimberly Jacoby-Morris
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  2. George H. Patterson
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Corresponding author

Correspondence to George H. Patterson .

Editor information

Editors and Affiliations

  1. NIAID, National Institutes of Health, Rockville, MD, USA

    Joseph Brzostowski

  2. NIAID, National Institutes of Health, Rockville, MD, USA

    Haewon Sohn

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© 2021 This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply and Springer Nature US

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Jacoby-Morris, K., Patterson, G.H. (2021). Choosing Fluorescent Probes and Labeling Systems. In: Brzostowski, J., Sohn, H. (eds) Confocal Microscopy. Methods in Molecular Biology, vol 2304. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1402-0_2

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  • DOI: https://doi.org/10.1007/978-1-0716-1402-0_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1401-3

  • Online ISBN: 978-1-0716-1402-0

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