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The Chicken Egg Chorioallantoic Membrane (CAM) Model as an In Vivo Method for the Investigation of the Invasion and Metastasis Cascade of Malignant Tumor Cells

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Metastasis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2294))

Abstract

The CAM model enables an in vivo analysis of the individual sub-steps of the metastatic cascade like local invasion, intravasation, or the establishment of metastasis in particular organs. Incubated fertilized chicken eggs are inoculated with human tumor cells and further processed for up to 9–10 days. The invasion and metastasis of these cells is then detected quantitatively with high specificity and sensitivity by means of a PCR for human ALU sequences, using the genomic DNA isolated from distant portions of the CAM, as well as from diverse internal organs of the developing embryo.

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Acknowledgments

H.A. was supported by the Alfried Krupp von Bohlen und Halbach Foundation, Essen, the Deutsche Krebshilfe, Bonn (70112168), the Deutsche Forschungsgemeinschaft (DFG, grant number AL 465/9-1), the HEiKA Initiative (Karlsruhe Institute of Technology/University of Heidelberg collaborative effort), Dr. Hella-Bühler-Foundation, the DKFZ-MOST Cooperation, Heidelberg (grant number CA149), the HIPO/POP-Initiative for Personalized Oncology, Heidelberg (H032 and H027).

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Authors and Affiliations

  1. Department of Experimental Surgery – Cancer Metastasis, Medical Faculty Mannheim, Ruprecht Karls University of Heidelberg, Mannheim, Germany

    Jörg H. Leupold, Nitin Patil & Heike Allgayer

  2. Centre for Biomedicine and Medical Technology Mannheim (CBTM), Medical Faculty Mannheim, Ruprecht Karls University of Heidelberg, Mannheim, Germany

    Jörg H. Leupold, Nitin Patil & Heike Allgayer

Authors
  1. Jörg H. Leupold
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  2. Nitin Patil
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  3. Heike Allgayer
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Corresponding author

Correspondence to Heike Allgayer .

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Editors and Affiliations

  1. Translational Oncology of Solid Tumors Experimental and Clinical Research Center, Charité – Universitätsmedizin Berlin and Max-Delbrück-Center for Molecular Medicine, Berlin, Germany

    Ulrike S. Stein

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Leupold, J.H., Patil, N., Allgayer, H. (2021). The Chicken Egg Chorioallantoic Membrane (CAM) Model as an In Vivo Method for the Investigation of the Invasion and Metastasis Cascade of Malignant Tumor Cells. In: Stein, U.S. (eds) Metastasis. Methods in Molecular Biology, vol 2294. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1350-4_2

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  • DOI: https://doi.org/10.1007/978-1-0716-1350-4_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1349-8

  • Online ISBN: 978-1-0716-1350-4

  • eBook Packages: Springer Protocols

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