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Quantifying the Affinity of Trypanosoma cruzi RPA-1 to the Single-Stranded DNA Overhang of the Telomere Using Surface Plasmon Resonance

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Single Stranded DNA Binding Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2281))

Abstract

Surface plasmon resonance (SPR) biosensors provide real-time binding affinity measurements between a pair of biomolecules, characterizing its interaction dynamics. An example of Trypanosoma cruzi’s RPA-1 and a single-stranded DNA telomere sequence is presented with detailed guidelines and fundamentals for SPR technology.

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Acknowledgments

This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 2013/07467-1; 2016/50050-2; 2017/07693-2) and Conselho Nacional de Pesquisa e Desenvolvimento (CNPq 306199/2018-1; 870219/1997-9).

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Authors and Affiliations

  1. Cell Cycle Laboratory, Butantan Institute, São Paulo, SP, Brazil

    Marcela de Oliveira Vitarelli & Maria Carolina Elias

  2. Center of Toxins, Immune Response and Cell Signaling (CeTICS), Butantan Institute, São Paulo, SP, Brazil

    Marcela de Oliveira Vitarelli & Maria Carolina Elias

Authors
  1. Marcela de Oliveira Vitarelli
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  2. Maria Carolina Elias
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Corresponding author

Correspondence to Maria Carolina Elias .

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Editors and Affiliations

  1. Jaboticabal, Brazil

    Marcos T. Oliveira

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de Oliveira Vitarelli, M., Elias, M.C. (2021). Quantifying the Affinity of Trypanosoma cruzi RPA-1 to the Single-Stranded DNA Overhang of the Telomere Using Surface Plasmon Resonance. In: Oliveira, M.T. (eds) Single Stranded DNA Binding Proteins. Methods in Molecular Biology, vol 2281. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1290-3_13

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  • DOI: https://doi.org/10.1007/978-1-0716-1290-3_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1289-7

  • Online ISBN: 978-1-0716-1290-3

  • eBook Packages: Springer Protocols

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