Abstract
Aldehydes are abundantly present in tobacco smoke and in urban air pollution and are endogenously generated as products of the lipid peroxidation process. These molecules can react with DNA bases forming mutagenic exocyclic adducts, which have been used as biomarkers of aldehyde exposure and as potential tools for the study of inflammation, metal storage diseases, neurodegenerative disorders, and cancer. High-performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS) provides a highly precise, specific and ultrasensitive method for the detection of exocyclic DNA adducts. Here we present and describe a validated micro-HPLC-Electro Spray Ionization (ESI)-MS/MS method for the quantification of 1,N2-propanodGuo, an adduct produced following the reaction between 2′-deoxyguanosine and acetaldehyde or crotonaldehyde.
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Acknowledgments
FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo, No. 2012/12663-1, CEPID Redoxoma No. 2013/07937-8), CNPq (Conselho Nacional para o Desenvolvimento Científico e Tecnológico, No. 302120/2018-1 and No. 159068/2014-2), PRPUSP (Pro-Reitoria de Pesquisa da Universidade de São Paulo, NAP Redoxoma No. 2011.1.9352.1.8).
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Sanchez, A.B., Garcia, C.C.M., Di Mascio, P., Medeiros, M.H.G. (2021). Detection of DNA Adduct Formation in Rat Lungs by a Micro-HPLC/MS/MS Approach. In: Santiago-Cardona, P.G. (eds) Lung Cancer. Methods in Molecular Biology, vol 2279. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1278-1_18
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DOI: https://doi.org/10.1007/978-1-0716-1278-1_18
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