Abstract
EPO has a complex glycosylation pattern with differently branched and charged glycans. A combination of hydrophilic interaction chromatography (HILIC) with weak anion exchange chromatography (WAX) enables highly orthogonal separation. Comprehensive 2D-LC analysis with HILIC in the first and WAX in the second dimension provides high resolution 2D chromatography together with simultaneous charge profiling. Meanwhile, multiple heart-cutting 2D-LC analysis combining WAX and HILIC separation provides a flexible alternative whereby the user can select multiple peaks to be analyzed in the second dimension and, moreover, run longer gradients in the second dimension.
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Schneider, S., Naegele, E., Krieger, S. (2021). Online 2D-LC for Complex N-Glycan Analysis from Biopharmaceuticals. In: Delobel, A. (eds) Mass Spectrometry of Glycoproteins. Methods in Molecular Biology, vol 2271. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1241-5_16
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DOI: https://doi.org/10.1007/978-1-0716-1241-5_16
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-1241-5
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