Abstract
B cells are primarily known for their capacity to differentiate into antibody-secreting cells (ASCs). ASCs are usually viewed as terminally differentiated cells sharing a unique phenotype. However, it lately became evident that ASCs exist in a variety of subsets differing by their lifespan, anatomic location, and immunological function. Thus, ASCs can exist as long-lived plasma cells (LLPC) that can persist for years in a nonproliferating state within particular niches in the bone marrow (BM), or as short-lived plasma cells (SLPC) that are primarily found in secondary lymphoid organs or inflamed tissues and wane upon the termination of the associated immune response. Another layer of ASC diversity was uncovered with the discovery of their capacity to produce various pro- or anti-inflammatory cytokines. Notably, a subset of natural regulatory plasma cells characterized by the distinctive expression of the inhibitory receptor lymphocyte activation gene (LAG)-3 and a unique capacity to produce interleukin (IL)-10 upon stimulation was recently identified. Here, we describe how to immunophenotypically characterize murine plasma cells as well as how to isolate them using cell sorting, with a special focus on these recently described natural regulatory plasma cells.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Smith T, Cunningham-Rundles C (2019) Primary B-cell immunodeficiencies. Hum Immunol 80(6):351–362. https://doi.org/10.1016/j.humimm.2018.10.015
Nutt SL, Hodgkin PD, Tarlinton DM, Corcoran LM (2015) The generation of antibody-secreting plasma cells. Nat Rev Immunol 15(3):160–171. https://doi.org/10.1038/nri3795
Oracki SA, Walker JA, Hibbs ML, Corcoran LM, Tarlinton DM (2010) Plasma cell development and survival. Immunol Rev 237(1):140–159. https://doi.org/10.1111/j.1600-065X.2010.00940.x
Chang HD, Tokoyoda K, Hoyer B, Alexander T, Khodadadi L, Mei H, Dorner T, Hiepe F, Burmester GR, Radbruch A (2019) Pathogenic memory plasma cells in autoimmunity. Curr Opin Immunol 61:86–91. https://doi.org/10.1016/j.coi.2019.09.005
Radbruch A, Muehlinghaus G, Luger EO, Inamine A, Smith KG, Dorner T, Hiepe F (2006) Competence and competition: the challenge of becoming a long-lived plasma cell. Nat Rev Immunol 6(10):741–750. https://doi.org/10.1038/nri1886
Amanna IJ, Carlson NE, Slifka MK (2007) Duration of humoral immunity to common viral and vaccine antigens. N Engl J Med 357(19):1903–1915. https://doi.org/10.1056/NEJMoa066092
Shen P, Fillatreau S (2015) Antibody-independent functions of B cells: a focus on cytokines. Nat Rev Immunol 15(7):441–451. https://doi.org/10.1038/nri3857
Fillatreau S, Sweenie CH, McGeachy MJ, Gray D, Anderton SM (2002) B cells regulate autoimmunity by provision of IL-10. Nat Immunol 3(10):944–950. https://doi.org/10.1038/ni833
Lino AC, Dang VD, Lampropoulou V, Welle A, Joedicke J, Pohar J, Simon Q, Thalmensi J, Baures A, Fluhler V, Sakwa I, Stervbo U, Ries S, Jouneau L, Boudinot P, Tsubata T, Adachi T, Hutloff A, Dorner T, Zimber-Strobl U, de Vos AF, Dahlke K, Loh G, Korniotis S, Goosmann C, Weill JC, Reynaud CA, Kaufmann SHE, Walter J, Fillatreau S (2018) LAG-3 inhibitory receptor expression identifies immunosuppressive natural regulatory plasma cells. Immunity 49(1):120–133 e129. https://doi.org/10.1016/j.immuni.2018.06.007
Acknowledgments
V.D.D is supported by Vinmec grant ISC.19.11. S.F. lab is supported by ERC PREG-LAB 647696, AXA Chair Translational Immunology, Chair of Excellence (University Paris Descartes), Agence Nationale pour la Recherche (ANR), INSERM, and CNRS. DFG grant LI3540/1-1 to ACL.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Dang, V.D., Fillatreau, S., Lino, A.C. (2021). Purification and Immunophenotypic Characterization of Murine Plasma Cells. In: Mion, F., Tonon, S. (eds) Regulatory B Cells. Methods in Molecular Biology, vol 2270. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1237-8_3
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1237-8_3
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1236-1
Online ISBN: 978-1-0716-1237-8
eBook Packages: Springer Protocols