Abstract
We describe the protocol for the efficient in vitro differentiation of human neural stem cells (NSCs) from human-induced pluripotent stem cells (iPS cells). NSCs differentiate via neural epithelial progenitors enabling the analysis of early neuronal development. They represent neural progenitor cells, which are capable of differentiating into neurons and glia.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Takahashi K, Tanabe K, Ohnuki M et al (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131(5):861–872. https://doi.org/10.1016/j.cell.2007.11.019
Yu J, Hu K, Smuga-Otto K et al (2009) Human induced pluripotent stem cells free of vector and transgene sequences. Science 324(5928):797–801. https://doi.org/10.1126/science.1172482
Foja S, Jung M, Harwardt B et al (2013) Hypoxia supports reprogramming of mesenchymal stromal cells via induction of embryonic stem cell-specific microRNA-302 cluster and pluripotency-associated genes. Cell Reprogram 15(1):68–79. https://doi.org/10.1089/cell.2012.0037
Jung M, Rujescu D (2016) Translational medicine: from disease- and patient-specific stem cell research to clinical trials and back again. Eur Arch Psychiatry Clin Neurosci 266(8):679–680. https://doi.org/10.1007/s00406-016-0742-1
Stoddard-Bennett T, Reijo Pera R (2019) Treatment of Parkinson’s disease through personalized medicine and induced pluripotent stem cells. Cells 8(1). https://doi.org/10.3390/cells8010026
Savage JE, Jansen PR, Stringer S et al (2018) Genome-wide association meta-analysis in 269,867 individuals identifies new genetic and functional links to intelligence. Nat Genet 50(7):912–919. https://doi.org/10.1038/s41588-018-0152-6
Sims R, van der Lee SJ, Naj AC et al (2017) Rare coding variants in PLCG2, ABI3, and TREM2 implicate microglial-mediated innate immunity in Alzheimer’s disease. Nat Genet 49(9):1373–1384. https://doi.org/10.1038/ng.3916
Pardinas AF, Holmans P, Pocklington AJ et al (2018) Common schizophrenia alleles are enriched in mutation-intolerant genes and in regions under strong background selection. Nat Genet 50(3):381–389. https://doi.org/10.1038/s41588-018-0059-2
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Jung, M., Schiller, J., Hartmann, C., Pfeifer, J., Rujescu, D. (2021). Generation of Neural Stem Cells from Pluripotent Stem Cells for Characterization of Early Neuronal Development. In: Stock, P., Christ, B. (eds) In Vitro Models for Stem Cell Therapy. Methods in Molecular Biology, vol 2269. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1225-5_16
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1225-5_16
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1224-8
Online ISBN: 978-1-0716-1225-5
eBook Packages: Springer Protocols