Abstract
Chondrocytes are the only cell type in cartilage. The dense cartilage extracellular matrix surrounding the chondrocytes makes isolating these cells a complex and lengthy task that subjects the cells to harsh conditions. Protocols to isolate expand and maintain these cells have been improved over the years, providing ways to obtain viable cells for tissue engineering and clinical applications. Here we describe a method to obtain populations of chondrocytes that are able to expand and maintain a native-like phenotype.
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Acknowledgments
This work was funded, in part, by grants from the National Institutes of Health P41 EB021911, R01 AR050208, and P01 AR053622.
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Somoza, R.A., Welter, J.F. (2021). Isolation of Chondrocytes from Human Cartilage and Cultures in Monolayer and 3D. In: Haqqi, T.M., Lefebvre, V. (eds) Chondrocytes. Methods in Molecular Biology, vol 2245. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1119-7_1
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DOI: https://doi.org/10.1007/978-1-0716-1119-7_1
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