Abstract
The intestine is often examined histologically in connection with allergies and in search for pathological changes. To be able to examine the intestine histologically with a microscope, it must be sampled and processed correctly. For microscopic analysis, the samples have to be cut into thin sections, stained, and mounted on slides. Since it is not possible to cut fresh samples without damaging them, they must first be fixed. The most common method, which is described herein, is the fixation in formalin with subsequent embedding in paraffin and staining of the slides with hematoxylin and eosin (H&E). Hematoxylin solutions (in this case Mayer’s hemalum solution) stain the acidic components of the cell, i.e., cell nuclei, blue. The staining with eosin gives a pink staining of cytoplasm. This chapter describes the method of processing intestinal tissue for paraffin-embedding, sectioning, and staining with H&E. Tissue processing can be done in tissue processing machines or manually. We describe the manual processing that is often used for smaller batches of samples.
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References
McKenzie C, Tan J, Macia L, Mackay CR (2017) The nutrition-gut microbiome-physiology axis and allergic diseases. Immunol Rev 278:277–295. https://doi.org/10.1111/imr.12556
Bancroft JD (2008) Theory and practice of histological techniques, 6th edn. Churchill Livingstone, London
Mulisch M, Welsch U (2010) Romeis—Mikroskopische Technik, 18th edn. Springer Spektrum, Berlin
Clarke EL, Treanor D (2017) Colour in digital pathology: a review. Histopathology 70:153–163. https://doi.org/10.1111/his.13079
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Rieger, J., Pelckmann, LM., Drewes, B. (2021). Preservation and Processing of Intestinal Tissue for the Assessment of Histopathology. In: Nagamoto-Combs, K. (eds) Animal Models of Allergic Disease. Methods in Molecular Biology, vol 2223. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1001-5_18
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DOI: https://doi.org/10.1007/978-1-0716-1001-5_18
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1000-8
Online ISBN: 978-1-0716-1001-5
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