Abstract
In eukaryotes, the maturation of the 3′ ends of most transcripts involves cleavage and polyadenylation steps in the nucleus. While I was working in the group of Katherine Borden at the University of Montréal, we reported that the eukaryotic translation initiation factor 4E (eIF4E) promotes the 3′ end cleavage of specific RNAs. Here, I describe how we monitored this specific maturation pathway using subcellular fractionation, quantitative RT-PCR, and an in vitro cleavage assay with the nuclear fraction.
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Acknowledgments
I gratefully acknowledge Pr. Katherine Borden for the opportunity to perform this work in her laboratory at University of Montréal (Canada) as well as Dr. Marc Boudvillain for critical reading and editing of this manuscript.
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Delaleau, M. (2021). Monitoring eIF4E-Dependent Nuclear 3′ End mRNA Cleavage. In: Boudvillain, M. (eds) RNA Remodeling Proteins. Methods in Molecular Biology, vol 2209. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0935-4_22
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DOI: https://doi.org/10.1007/978-1-0716-0935-4_22
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Publisher Name: Humana, New York, NY
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