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Phosphoproteomic Analysis of Plant Membranes

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Arabidopsis Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2200))

Abstract

Mass spectrometry (MS) is a powerful tool to investigate plant phosphorylation dynamics on a system-wide scale (phosphoproteomics). Plant membrane phosphoproteomics enables elucidating regulatory patterns in membranes, such as kinase-target relationships in different signaling pathways. Here, we present “ShortPhos,” an efficient and simple phosphoproteomics protocol for research on plant membrane proteins, which allows fast and efficient identification and quantification of phosphopeptides from small amounts of starting plant material and/or membrane proteins. This method improves upon the efficiency of plant membrane phosphoproteomics profiling and can be applied to the study of membrane-based signaling networks.

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Author information

Authors and Affiliations

  1. Department of Plant Systems Biology, University of Hohenheim, Stuttgart, Germany

    Lin Xi, Waltraud X. Schulze & Xu Na Wu

  2. School of Life Sciences, Center for Life Sciences, Yunnan University, Kunming, China

    Xu Na Wu

Authors
  1. Lin Xi
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  2. Waltraud X. Schulze
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  3. Xu Na Wu
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Corresponding author

Correspondence to Xu Na Wu .

Editor information

Editors and Affiliations

  1. Centro Nacional de Biotecnología, CSIC Centro Nacional de Biotecnología, Madrid, Spain

    Jose J. Sanchez-Serrano

  2. Centro de Investigaciones Biologicas, CSIC Madrid Centro de Investigaciones Biologicas, Madrid, Spain

    Julio Salinas

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Xi, L., Schulze, W.X., Wu, X.N. (2021). Phosphoproteomic Analysis of Plant Membranes. In: Sanchez-Serrano, J.J., Salinas, J. (eds) Arabidopsis Protocols . Methods in Molecular Biology, vol 2200. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0880-7_22

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  • DOI: https://doi.org/10.1007/978-1-0716-0880-7_22

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0879-1

  • Online ISBN: 978-1-0716-0880-7

  • eBook Packages: Springer Protocols

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