Abstract
To understand how the brain functions we need to understand the properties of its constituent cells. Whole-cell patch-clamp recordings of neurons have enabled studies of their intrinsic electrical properties as well as their synaptic connectivity within neural circuits. Recent technological advances have now made it possible to combine this with a sampling of their transcriptional profile. Here we provide a detailed description how to combine whole-cell patch-clamp recordings of neurons in brain slices followed by extraction of their cytoplasm suitable for single-cell RNA sequencing and analysis.
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Acknowledgments
This work was supported by a MRC Career Development Award (MR/M009599/1) to TJE.
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Mahfooz, K., Ellender, T.J. (2021). Combining Whole-Cell Patch-Clamp Recordings with Single-Cell RNA Sequencing. In: Dallas, M., Bell, D. (eds) Patch Clamp Electrophysiology. Methods in Molecular Biology, vol 2188. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0818-0_9
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DOI: https://doi.org/10.1007/978-1-0716-0818-0_9
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0817-3
Online ISBN: 978-1-0716-0818-0
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