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Generation of Functional Genetic Study Models in Zebrafish Using CRISPR-Cas9

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Cancer Cell Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2174))

Abstract

CRISPR-Cas9 is a method for genome editing that can be used efficiently for in vivo applications; the basic implementation of this method is used to generate genome site-directed sequence eliminations. Here we describe a protocol for genome editing using CRISPR-Cas9 in zebrafish (Danio rerio) one-cell embryos.

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Acknowledgements

This work is supported by a grant from Consejo Nacional de Ciencia y Tecnología (Fronteras de la Ciencia 2015-290) and Fundación Miguel Alemán and by Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica (PAPIIT IN203917) to FR-T. HNN-M and CAP-A are doctoral students from Programa de Doctorado en Ciencias Biomédicas (661245 and 390924), Universidad Nacional Autónoma de México and GT-U is a doctoral student from the Programa de Doctorado en Ciencias Bioquímicas (630552), all of them received fellowships from Consejo Nacional de Ciencia y Tecnología.

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Authors and Affiliations

  1. Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico

    Francisco Carmona-Aldana, Hober N. Nuñez-Martinez, Carlos A. Peralta-Alvarez, Gustavo Tapia-Urzua & Félix Recillas-Targa

Authors
  1. Francisco Carmona-Aldana
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  2. Hober N. Nuñez-Martinez
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  3. Carlos A. Peralta-Alvarez
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  4. Gustavo Tapia-Urzua
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  5. Félix Recillas-Targa
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Corresponding author

Correspondence to Félix Recillas-Targa .

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Editors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico

    Martha Robles-Flores

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Carmona-Aldana, F., Nuñez-Martinez, H.N., Peralta-Alvarez, C.A., Tapia-Urzua, G., Recillas-Targa, F. (2021). Generation of Functional Genetic Study Models in Zebrafish Using CRISPR-Cas9. In: Robles-Flores, M. (eds) Cancer Cell Signaling. Methods in Molecular Biology, vol 2174. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0759-6_16

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  • DOI: https://doi.org/10.1007/978-1-0716-0759-6_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0758-9

  • Online ISBN: 978-1-0716-0759-6

  • eBook Packages: Springer Protocols

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