Abstract
The dynamin-related proteins (DRPs) are self-assembling membrane remodeling machines that are indispensable for fundamental cellular trafficking and homeostatic processes. We describe in this chapter protocols developed in our laboratory for purification of full-length and minimal constructs of Chaetomium thermophilum Vps1, the model fungal DRP, using mammalian and Escherichia coli expression systems.
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Acknowledgement
We thank Tyler M. Brady and Bryan A. Tornabene for assistance with protein purification. This work was supported by the National Institutes of Health grant GM120102 (M. G. J. Ford).
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Varlakhanova, N.V., Ford, M.G.J. (2020). Purification of the Dynamin-Related Protein Vps1 Using Mammalian and Bacterial Expression Systems. In: Ramachandran, R. (eds) Dynamin Superfamily GTPases. Methods in Molecular Biology, vol 2159. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0676-6_2
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DOI: https://doi.org/10.1007/978-1-0716-0676-6_2
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