Abstract
Dynamin-like protein 1 (Drp1) is the master regulator of mitochondrial fission. Drp1 translocates from the cytosol to the mitochondrial outer membrane to execute the scission process. Here we describe an immunofluorescence-based method to measure the mitochondrial translocation of Drp1 and quantify Drp1-related mitochondrial fission by labeling the mitochondrial import receptor subunit TOM20 in fixed cell culture.
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Hu, D., Qi, X. (2020). Quantifying Drp1-Mediated Mitochondrial Fission by Immunostaining in Fixed Cells. In: Ramachandran, R. (eds) Dynamin Superfamily GTPases. Methods in Molecular Biology, vol 2159. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0676-6_15
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DOI: https://doi.org/10.1007/978-1-0716-0676-6_15
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0675-9
Online ISBN: 978-1-0716-0676-6
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