Abstract
Plant cells are heavily compartmentalized, and metabolite concentrations in the various compartments differ significantly. Thus, determination of metabolite abundance in whole-cell extracts may be misleading, when the role of a compound in plant freezing tolerance shall be evaluated. Here, we describe a method for the separation of the largest compartments of plant cells, the vacuole, plastid, and cytosol. With more elaborate analysis, this method can be expanded to also resolve mitochondria and other compartments.
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Acknowledgments
This work was supported by grant HE 3087/10-2 from the German Science Foundation to AGH.
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Hoermiller, I.I., Nägele, T., Heyer, A.G. (2020). Subcellular Compartmentation of Metabolites Involved in Cold Acclimation. In: Hincha, D., Zuther, E. (eds) Plant Cold Acclimation. Methods in Molecular Biology, vol 2156. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0660-5_18
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DOI: https://doi.org/10.1007/978-1-0716-0660-5_18
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-0660-5
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