Abstract
Mouse embryonic stem cells (mESC) have the ability to self-renew due to their rapid proliferation and high telomerase activity while maintaining their pluripotency. Depending on the environment, mESC can differentiate into a broad range of cell types. These characteristics have established mESC as a tool for modeling human disease, genetic engineering, lineage specificity, stem cell-based therapies, and tissue regeneration. Here we describe a protocol for mESC expansion and differentiation.
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References
Evans MJ, Kaufman MH (1981) Establishment in culture of pluripotential cells from mouse embryos. Nature 292(5819):154–156
Martin GR (1981) Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci U S A 78(12):7634–7638
Smith AG (2003) Embryo-derived stem cells: of mice and men. Annu Rev Cell Dev Biol 17(1):435–462. https://doi.org/10.1146/annurev.cellbio.17.1.435
Bradley A, Evans M, Kaufman MH, Robertson E (1984) Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines. Nature 309(5965):255–256
Keller GM (1995) In vitro differentiation of embryonic stem cells. Curr Opin Cell Biol 7(6):862–869
Doetschman TC, Eistetter H, Katz M, Schmidt W, Kemler R (1985) The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J Embryol Exp Morphol 87(1):27–45
Nakano T, Kodama H, Honjo T (1994) Generation of lymphohematopoietic cells from embryonic stem cells in culture. Science 265(5175):1098–1101
Nishikawa SI, Nishikawa S, Hirashima M, Matsuyoshi N, Kodama H (1998) Progressive lineage analysis by cell sorting and culture identifies FLK1+VE-cadherin+ cells at a diverging point of endothelial and hemopoietic lineages. Development 125(9):1747–1757
Williams RL, Hilton DJ, Pease S, Willson TA, Stewart CL, Gearing DP et al (1988) Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells. Nature 336(6200):684–687
Nichols J, Zevnik B, Anastassiadis K, Niwa H, Klewe-Nebenius D, Chambers I et al (1998) Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell 95(3):379–391
Niwa H, Miyazaki J-I, Smith AG (2000) Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat Genet 24(4):372–376
Avilion AA, Nicolis SK, Pevny LH, Perez L, Vivian N, Lovell-Badge R (2003) Multipotent cell lineages in early mouse development depend on SOX2 function. Genes Dev 17(1):126–140
Chambers I, Colby D, Robertson M, Nichols J, Lee S, Tweedie S et al (2003) Functional expression cloning of nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 113(5):643–655
Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K et al (2003) The homeoprotein nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell 113(5):631–642
Wray J, Kalkan T, Gomez-Lopez S, Eckardt D, Cook A, Kemler R et al (2011) Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation. Nat Cell Biol 13(7):838–845
Ying Q-L, Wray J, Nichols J, Batlle-Morera L, Doble B, Woodgett J et al (2008) The ground state of embryonic stem cell self-renewal. Nature 453(7194):519–523
Mulas C, Kalkan T, von Meyenn F, Leitch HG, Nichols J, Smith A (2019) Defined conditions for propagation and manipulation of mouse embryonic stem cells. Development 146(6):dev173146
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Kioussi, C. (2020). Culturing and Manipulating Mouse Embryonic Stem Cells. In: Kioussi, C. (eds) Stem Cells and Tissue Repair . Methods in Molecular Biology, vol 2155. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0655-1_1
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DOI: https://doi.org/10.1007/978-1-0716-0655-1_1
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0654-4
Online ISBN: 978-1-0716-0655-1
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