Abstract
Innate lymphoid cells (ILCs) are important regulators of the early responses to infection at mucosal barriers, including the intestine. Recently, we have shown that specific ILC3 subsets protect against enteric bacterial pathogens. Here, we describe a mouse model of oral infection by Yersinia enterocolitica (Y. enterocolitica) and several different methodologies to assess the severity of the infection. We also detail how ILC3 subsets can be isolated from the mouse small intestine and transferred into recipient immune deficient mice to study the function of these ILCs in the small intestine.
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Acknowledgments
This work was supported by grants from the National Institutes of Health P01 DK46763, R01 AI61516, and MIST U01 AI125955 to M.K.; F32 AI140581 to D.A.G.; and National Research Foundation (NRF) of Korea 2013R1A1A2057931 to G.-Y.S. We thank the staff of the Microscopy & Histology Core (Zbigniew Mikulski and Angela Denn), Flow Cytometry Core and the Department of Laboratory Animal Care (DLAC) at LJI for excellent technical assistance, SDx Histopathology Inc., (Carlsbad, CA) for help with histology analysis.
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Seo, GY., Giles, D.A., Kronenberg, M. (2020). Bacterial Infection Allows for Functional Examination of Adoptively Transferred Mouse Innate Lymphoid Cell Subsets. In: Amarnath, S. (eds) Innate Lymphoid Cells . Methods in Molecular Biology, vol 2121. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0338-3_12
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DOI: https://doi.org/10.1007/978-1-0716-0338-3_12
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-0338-3
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