Abstract
Current treatment options are not providing an adequate solution for cartilage defects. Articular cartilage lesions in particular are not able to repair spontaneously and progressively degenerate with an arthrosic pattern. Aiming to solve this pressing medical need, xenotransplantation of porcine chondrocytes could be developed as a new therapeutic approach. Xenotransplantation is gaining much attention, thanks to the advances in animal genetic engineering and progress in the characterization of the rejection mechanisms that prevent long-term graft survival. In this regard, our team has identified various targets for intervention that should be tested in a meaningful animal model to prove their relevance in rejection of xenogeneic cartilage. To this end, we have recently established a discordant xenotransplantation model by injecting three million porcine articular chondrocytes (PAC) into the femorotibial joint of Lewis rats. This chapter describes this new model, which can be used to assess the immunoregulatory effect of a variety of strategies designed to inhibit rejection of xenogeneic PAC both at the humoral and cellular levels.
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References
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Acknowledgments
This work was funded by Ministerio de Ciencia e Innovación—Instituto de Salud Carlos III (PI11/02179) and the SLT002/16/00445 grant funded by the Department of Health of the Generalitat de Catalunya, both granted to CC, and cofunded by FEDER funds—a way to build Europe. IDIBELL benefits from the support of CERCA. M.P.-C. was supported by an IDIBELL fellowship.
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Marquina, M., Pérez-Cruz, M., Costa, C. (2020). Rat Model of Intra-articular Chondrocyte Xenotransplantation. In: Costa, C. (eds) Xenotransplantation. Methods in Molecular Biology, vol 2110. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0255-3_17
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DOI: https://doi.org/10.1007/978-1-0716-0255-3_17
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