Abstract
Among the different secondary structures that DNA can adopt, G-quadruplex is a noncanonical form that has recently started to garner attention about the possible layers of regulation they could introduce in cellular processes. Here, we outline how the presence of G-quadruplexes can be probed in legumes and other plant genomes. This chapter describes various in silico approaches that can be utilized to identify putative G-quadruplex forming sequences (GQSes) and validate their formation through in vitro experimental approaches.
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Acknowledgments
SS and KG acknowledge Shiv Nadar University for Research Fellowship and TM acknowledges SNU for funding OUR project. RG acknowledges Shiv Nadar University and Early Career Research Award from the Science and Engineering Research Board, Government of India, for funding support.
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Singh, S., Mathur, T., Gupta, K., Garg, R. (2020). Methods for Identification and Validation of G-Quadruplex Sequences in Legumes. In: Jain, M., Garg, R. (eds) Legume Genomics. Methods in Molecular Biology, vol 2107. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0235-5_13
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DOI: https://doi.org/10.1007/978-1-0716-0235-5_13
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