Abstract
Target enrichment sequencing (TES) is a powerful approach to deep-sequencing the exome or genomic regions of interest with great depth. Although successfully and widely adopted in many plant species, TES is currently applied for genotyping of only a couple legume species. Here we describe an in-solution probe capture based method for application of TES in legumes. The topics cover probe design, library preparation, probe hybridization, as well as bioinformatic analysis for evaluation of target capture efficiency and identifying single nucleotide polymorphisms using generated sequencing data.
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This work was supported by the Florida Peanut Producers Association and National Peanut Board.
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Peng, Z., Paudel, D., Wang, L., Luo, Z., You, Q., Wang, J. (2020). Methods for Target Enrichment Sequencing via Probe Capture in Legumes. In: Jain, M., Garg, R. (eds) Legume Genomics. Methods in Molecular Biology, vol 2107. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0235-5_10
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DOI: https://doi.org/10.1007/978-1-0716-0235-5_10
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