Abstract
Only a limited number of large-scale protocols describe the production of mature skeletal muscle fibers from human induced pluripotent stem cells (hiPSCs). Here we describe a novel procedure for simultaneous differentiation of hiPSC into muscle cells and motor neurons, that generates innervated and contractile multinucleated skeletal muscle fibers with sarcomeric organization. Our protocol permits the production of expandable skeletal muscle progenitor cells and mature skeletal muscle fibers that can be used for the exploration of skeletal muscle differentiation for basic research, disease modeling, and drug discovery.
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Acknowledgments
This study was funded by Association Française contre les Myopathies (AFM, TRIM-RD program) and Region Provence Alpes Côte d’Azur (Cladimus project, 2018-06436). M.D. is the recipient of a fellowship from the French Ministry of Research and Higher Education. The project leading to this chapter has received funding from the Excellence Initiative of Aix-Marseille University-A*Midex, a French “investissement d’avenir programme” AMX-19-IET-007 through the Marseille Maladies Rares (MarMaRa) Institute. Mégane Delourme and Natacha Broucqsault contributed equally to this work.
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Delourme, M., Broucqsault, N., Mazaleyrat, K., Magdinier, F. (2020). Production of Innervated Skeletal Muscle Fibers Using Human Induced Pluripotent Stem Cells. In: Nagy, A., Turksen, K. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 2454. Humana, New York, NY. https://doi.org/10.1007/7651_2020_334
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DOI: https://doi.org/10.1007/7651_2020_334
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Print ISBN: 978-1-0716-2118-9
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