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The Isolation and Manufacture of GMP-Grade Bone Marrow Stromal Cells from Bone Specimens

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Stem Cells and Good Manufacturing Practices

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2286))

Abstract

Bone marrow stromal cells (BMSCs, also known as bone marrow mesenchymal stem cells) are a plastic-adherent heterogeneous cell population that contain inherent skeletal progenitors and a subset of multipotential skeletal stem cells (SSCs). Application of BMSCs in therapeutic protocols implies its isolation and expansion under good manufacturing practices (GMP). Here we describe the procedures we have found to successfully generate practical BMSCs numbers, with preserved biological potency.

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Authors and Affiliations

  1. Research Division, National Institute of Traumatology and Orthopedics, Rio de Janeiro, Brazil

    Rhayra B. Dias

  2. Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

    Rhayra B. Dias & Danielle C. Bonfim

Authors
  1. Rhayra B. Dias
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  2. Danielle C. Bonfim
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Corresponding author

Correspondence to Danielle C. Bonfim .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Dias, R.B., Bonfim, D.C. (2021). The Isolation and Manufacture of GMP-Grade Bone Marrow Stromal Cells from Bone Specimens. In: Turksen, K. (eds) Stem Cells and Good Manufacturing Practices. Methods in Molecular Biology, vol 2286. Humana, New York, NY. https://doi.org/10.1007/7651_2020_319

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  • DOI: https://doi.org/10.1007/7651_2020_319

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1326-9

  • Online ISBN: 978-1-0716-1327-6

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