Abstract
Recently, we reported our methodology for isolating plasma membrane and lysosome from eukaryotic cell using superparamagnetic nanoparticles (SPMNPs). Here in this article, we report a step-by-step protocol for synthesis of hybrid gold nanoparticle (AuNP), surface functionalization of AuNPs on superparamagnetic nanoparticles (SPMNPs), and potential use of hybrid AuNP-SPMNP for efficient coupling of biomolecules.
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References
Thimiri Govinda Raj DB et al (2011) A novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes. Mol Syst Biol 7:541
Sun S, Zeng H (2002) Size-controlled synthesis of magnetite nanoparticles. J Am Chem Soc 124(28):8204–8205
Dubertret B, Skourides P, Norris DJ, Noireaux V, Brivanlou AH, Libchaber A (2002) In vivo imaging of quantum dots encapsulated in phospholipid micelles. Science 298(5599):1759–1762
Raj DBTG (2011) Superparamagnetic nanoparticle based (10nm) isolation of plasma membrane for high resolution mass spec. analysis of the proteome, lipidome and glycome. In: Semiconductor physics section. KU Leuven, Leuven
Thimiri Govinda Raj DB, Khan NA (2018) Surface functionalization dependent subcellular localization of superparamagnetic nanoparticle in plasma membrane and endosome. Nano Converg 5:4
Thimiri Govinda Raj DB, Khan NA (2019) Protocol for eukaryotic plasma membrane isolation using superparamagnetic nanoparticles. J Magn Magn Mater 476:628–631. https://doi.org/10.1016/j.jmmm.2017.12.070
Thimiri Govinda Raj DB, Khan NA (2017) Designer nanoparticle: nano-biotechnology tool for cell biology. Nano Converg 3(1):22
Thimiri Govinda Raj DB, Khan NA, Venkatachalam S, Chu DT (2019) Step by step protocol for superparamagnetic nanoparticle based plasma membrane isolation from eukaryotic cell. Methods Mol Biol. https://doi.org/10.1007/7651_2019_211
Thimiri Govinda Raj DB, Khan NA, Venkatachalam S, Chu DT, Arumugam S (2019) Step by step protocol for superparamagnetic nanoparticle based endosome and lysosome isolation from eukaryotic cell. Methods Mol Biol. https://doi.org/10.1007/7651_2019_212
Thimiri Govinda Raj DB, Khan NA (2018) Synthesis of hybrid gold nanoparticles functionalized superparamagnetic nanoparticles. Micro Nano Lett 13(3):292. https://doi.org/10.1049/mnl.2017.0574
Acknowledgments
This work was supported by Envirotransgene® Global, Chennai, India, and Deepak Thimiri Consulting Group (DTCG®), Chennai, India. Authors thank the infrastructural support from Bannari Amman Institute of Technology, India.
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Thimiri Govinda Raj, D.B., Khan, N.A., Venkatachalam, S., Arumugam, S., Palaniswamy, S. (2019). Synthesis of Hybrid Gold Nanoparticle (AuNP) Functionalized Superparamagnetic Nanoparticles (SPMNPs) for Efficient Coupling of Biomolecules. In: Turksen, K. (eds) Stem Cell Nanotechnology. Methods in Molecular Biology, vol 2125. Humana, New York, NY. https://doi.org/10.1007/7651_2019_254
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DOI: https://doi.org/10.1007/7651_2019_254
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0359-8
Online ISBN: 978-1-0716-0360-4
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