Abstract
Autophagy is increasingly identified as a central player in many cellular activities from cell proliferation to cell division, migration, and differentiation. However, it is also considered as a double-edged sword in cancer biology which either promotes oncogenesis/invasion or sensitizes the tumor cells to chemotherapy induced apoptosis. Recent investigations have provided direct evidence for regulation of cellular phenotype via autophagy pathway. One of the most important types of phenotype conversion is Epithelial-Mesenchymal-Transition (EMT), resulting in alteration of epithelial cell properties to a more mesenchymal form. In the current chapter, we provide a method which is established and being used in our laboratory for detection of autophagy and EMT in lung epithelial cells and show the involvement of autophagy in modulation of cellular phenotype.
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Acknowledgement
SG was supported by Health Science Centre Foundation General Operating Grant and University Collaborative Research Program. SS was supported by Health Science Centre Foundation General Operating Grant and University Collaborative Research Program and Mitacs Accelerate Postdoctoral Fellowship. JA was supported by Research Manitoba studentship award.
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Alizadeh, J., Shojaei, S., Sepanjnia, A., Hashemi, M., Eftekharpour, E., Ghavami, S. (2017). Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-small Cell Lung Cancer Cells. In: Turksen, K. (eds) Autophagy in Differentiation and Tissue Maintenance. Methods in Molecular Biology, vol 1854. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_84
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