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Cloning of Autophagy-Related MicroRNAs

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Autophagy in Differentiation and Tissue Maintenance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1854))

Abstract

Autophagy is a cellular survival pathway that is necessary for the degradation of cellular constituents such as long-lived proteins and damaged organelles. Conditions resulting in cellular stress such as starvation or hypoxia might activate autophagy. Being at the crossroads of various cellular response pathways, dysregulation of autophagy might result in pathological states including cancer and neurodegenerative diseases. Autophagy has also been shown to participate in stemness. MicroRNAs were introduced as novel regulators of autophagy, and accumulating results underlined the fact that they constituted an important layer of biological control mechanism on the autophagic activity.

MicroRNAs are protein noncoding small RNAs that control cellular levels of transcripts and proteins through posttrancriptional mechanisms. Novel miRNAs in human and mouse genomes are yet to be identified. Considering the emerging role of autophagy in health and disease, identification of novel autophagy-regulating miRNAs and determination of relations between miRNA expression and physiological and pathological conditions might contribute to a better understanding of mechanisms governing health and disease. High-throughput techniques were developed for miRNA profiling, yet for a thorough characterization and miRNA target determination, miRNA cloning remains as an important step. Here, we describe a modified miRNA cloning method for the characterization of novel autophagy-regulating miRNAs.

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Authors and Affiliations

  1. Faculty of Engineering and Natural Sciences, Molecular Biology, Genetics and Bioengineering Program, Sabanci University, Orhanli-Tuzla, Istanbul, 34956, Turkey

    Deniz Gulfem Ozturk, Muhammed Kocak & Devrim Gozuacik

  2. Center of Excellence for Functional Surfaces and Interfaces for Nano Diagnostics (EFSUN), Sabanci University, Orhanli-Tuzla, Istanbul, 34956, Turkey

    Devrim Gozuacik

Authors
  1. Deniz Gulfem Ozturk
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  2. Muhammed Kocak
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  3. Devrim Gozuacik
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Corresponding author

Correspondence to Devrim Gozuacik .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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© 2017 Springer Science+Business Media New York

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Ozturk, D.G., Kocak, M., Gozuacik, D. (2017). Cloning of Autophagy-Related MicroRNAs. In: Turksen, K. (eds) Autophagy in Differentiation and Tissue Maintenance. Methods in Molecular Biology, vol 1854. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_83

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  • DOI: https://doi.org/10.1007/7651_2017_83

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8747-4

  • Online ISBN: 978-1-4939-8748-1

  • eBook Packages: Springer Protocols

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