Abstract
In renal transplantation, the discovery of early urine biomarkers of graft lesions would be useful in helping physicians to improve patient care and minimize the use of invasive techniques such as biopsies. Over the last years, high-resolution mass spectrometry has been used extensively for the search of biomarkers in various biological fluids. Here we describe a procedure based on reverse-phase nano-HPLC, offline plate spotting, and MALDI-TOF and TOF/TOF applied in our laboratory for the search of natural peptides in urine samples from renal transplant patients.
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References
Pejcic M, Stojnev S, Stefanovic V (2010) Urinary proteomics—a tool for biomarker discovery. Ren Fail 32:259–268
Bestard O, Cruzado JM, la Franquesa M, Grinyo JM (2010) Biomarkers in renal transplantation. Curr Opin Organ Transplant 15:467–473
Alachkar N, Rabb H, Jaar BG (2011) Urinary biomarkers in acute kidney transplant dysfunction. Nephron Clin Pract 118:c173–c181
Rodríguez-Suárez E, Siwy J, Zürbig P, Mischak H (2014) Urine as a source for clinical proteome analysis: from discovery to clinical application. Biochim Biophys Acta 1(844):884–898
Ling XB, Mellins ED, Sylvester KG, Cohen HJ (2010) Urine peptidomics for clinical biomarker discovery. Adv Clin Chem 51:181–213
Metzger J, Schanstra JP, Mischak H (2009) Capillary electrophoresis-mass spectrometry in urinary proteome analysis: current applications and future developments. Anal Bioanal Chem 393:1431–1442
Mischak H, Coon JJ, Novak J, Weissinger EM, Schanstra JP, Dominiczak AF (2009) Capillary electrophoresis-mass spectrometry as a powerful tool in biomarker discovery and clinical diagnosis: an update of recent developments. Mass Spectrom Rev 28:703–724
Mischak H, Delles C, Klein J, Schanstra JP (2010) Urinary proteomics based on capillary electrophoresis—coupled mass spectrometry in kidney disease: discovery and validation of biomarkers, and clinical applications. Adv Chronic Kidney Dis 17:493–506
Ling XB, Sigdel TK, Lau K, Ying L, Lau I, Schilling J, Sarwal MM (2010) Integrative urinary peptidomics in renal transplantation identifies biomarkers for acute rejection. J Am Soc Nephrol 21:646–653
Mischak H, Schanstra JP (2011) CE-MS in biomarker discovery, validation, and clinical application. Proteomics Clin Appl 5:9–23
Siwy J, Mullen W, Golovko I, Franke J, Zurbig P (2011) Human urinary peptide database for multiple disease biomarker discovery. Proteomics Clin Appl 5:367–374
Court M, Selevsek N, Matondo M, Allory Y, Garin J, Masselon CD, Domon B (2011) Toward a standardized urine proteome analysis methodology. Proteomics 11:1160–1171
Benkali K, Marquet P, Rerolle JP, Le Meur Y, Gastinel L (2008) A new strategy for faster urinary biomarkers identification by Nano-LC-MALDI-TOF/TOF mass spectrometry. BMC Genomics 9:541
Quintana LF, Campistol JM, Alcolea MP, Banon-Maneus E, Sol-Gonzalez A, Cutillas PR (2009) Application of label-free quantitative peptidomics for the identification of urinary biomarkers of kidney chronic allograft dysfunction. Mol Cell Proteomics 8(1):658–1673
Mischak H, Kolch W, Aivaliotis M, Bouyssié D, Court M, Dihazi H, Dihazi GH, Franke J, Garin J, Gonzalez de Peredo A, Iphöfer A, Jänsch L, Lacroix C, Makridakis M, Masselon C, Metzger J, Monsarrat B, Mrug M, Norling M, Novak J, Pich A, Pitt A, Bongcam-Rudloff E, Siwy J, Suzuki H, Thongboonkerd V, Wang LS, Zoidakis J, Zürbig P, Schanstra JP, Vlahou A (2010) Comprehensive human urine standards for comparability and standardization in clinical proteome analysis. Proteomics Clin Appl 4:464–478
Sigdel TK, Nicora CD, Hsieh SC, Dai H, Qian WJ, Camp DG II, Sarwal MM (2014) Optimization for peptide sample preparation for urine peptidomics. Clin Proteomics 11:7
Sauvage FL, Gastinel LN, Marquet P (2012) Untargeted screening of urinary peptides with liquid chromatography coupled to hybrid linear-ion trap tandem mass spectrometry. J Chromatogr A 1(259):138–147
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Sauvage, FL., Passeron, S., Marquet, P. (2017). Untargeted Screening of Urinary Peptides Using Offline Nano-Liquid Chromatography: MALDI-TOF/TOF Mass Spectrometry. In: Sarwal, M., Sigdel, T. (eds) Tissue Proteomics. Methods in Molecular Biology, vol 1788. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_74
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DOI: https://doi.org/10.1007/7651_2017_74
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