Abstract
Immunoassays represent valuable and broadly used techniques for detection and quantification of proteins. Thanks to their high sensitivity, such techniques are powerful for analyzing growth factors, trophic factors, angiogenic factors, hormones, cytokines, chemokines, soluble receptors, and other proteins which play key roles in intercellular communication and operate as potent regulators of stem cell survival, proliferation, differentiation, or cell death. Multiplex immunological assays, in contrast to ELISA, offer simultaneous quantification of tens of proteins across multiple samples, and have been developed to save time, costs, and sample volumes. Among them, planar antibody microarrays and xMAP® bead-based assays have become particularly popular for characterization of proteins secreted by stem cells, as they are relatively easy, highly accurate, multiplex to a high degree and a broad spectrum of analytes can be measured. Here, we describe protocols for multiplex quantification of secreted proteins using Quantibody® microarrays (RayBiotech) and xMAP® assays (Luminex and its partners).
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Acknowledgement
This work was supported by the CHDI Foundation (ID 1035, A5378), the Technical Agency of the Czech Republic (TA01011466), the project EXAM from European Regional Development Fund CZ.1.05/2.1.00/03.0124 and by Institutional Research Concept Grant RVO 67985904.
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Valekova, I., Skalnikova, H.K., Jarkovska, K., Motlik, J., Kovarova, H. (2014). Multiplex Immunoassays for Quantification of Cytokines, Growth Factors, and Other Proteins in Stem Cell Communication. In: Turksen, K. (eds) Stem Cell Renewal and Cell-Cell Communication. Methods in Molecular Biology, vol 1212. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_94
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DOI: https://doi.org/10.1007/7651_2014_94
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Publisher Name: Humana Press, New York, NY
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