Abstract
Myocardial infarction is a leading cause of mortality and morbidity worldwide, and current treatments fail to address the underlying scarring and cell loss, which is a major cause of heart failure after infarction. The novel strategy, therapeutic angiogenesis and/or vasculogenesis with endothelial progenitor cells transplantation holds great promise to increase blood flow in ischemic areas, thus rebuild the injured heart and reverse the heart failure. Given the potential of self-renewal and differentiation into virtually all cell types, human embryonic stem cells (hESCs) may provide an alternate source of therapeutic cells by allowing the derivation of large numbers of endothelial cells for therapeutic angiogenesis and/or vasculogenesis of ischemic heart diseases. Moreover, to fully understand the fate of implanted hESCs or hESC derivatives, investigators need to monitor the motility of cells in living animals over time. In this chapter, we describe the application of bioluminescence reporter gene imaging to track the transplanted hESC-derived endothelial cells for treatment of myocardial infarction. The technology of inducing endothelial cells from hESCs will also be discussed.
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References
Li Z, Han Z, Wu JC (2009) Transplantation of human embryonic stem cell-derived endothelial cells for vascular diseases. J Cell Biochem 106(2):194–199. doi:10.1002/jcb.22003
Wollert KC, Drexler H (2005) Clinical applications of stem cells for the heart. Circ Res 96(2):151–163. doi:10.1161/01.RES.0000155333.69009.63
de Muinck ED, Thompson C, Simons M (2006) Progress and prospects: cell based regenerative therapy for cardiovascular disease. Gene Ther 13(8):659–671. doi:10.1038/sj.gt.3302680
Li Z, Wilson KD, Smith B, Kraft DL, Jia F, Huang M, Xie X, Robbins RC, Gambhir SS, Weissman IL, Wu JC (2009) Functional and transcriptional characterization of human embryonic stem cell-derived endothelial cells for treatment of myocardial infarction. PLoS One 4(12):e8443. doi:10.1371/journal.pone.0008443
Moon SH, Kim JS, Park SJ, Lee HJ, Do JT, Chung HM (2011) A system for treating ischemic disease using human embryonic stem cell-derived endothelial cells without direct incorporation. Biomaterials 32(27):6445–6455. doi:10.1016/j.biomaterials.2011.05.026
Yu J, Huang NF, Wilson KD, Velotta JB, Huang M, Li Z, Lee A, Robbins RC, Cooke JP, Wu JC (2009) nAChRs mediate human embryonic stem cell-derived endothelial cells: proliferation, apoptosis, and angiogenesis. PLoS One 4(9):e7040. doi:10.1371/journal.pone.0007040
Wang L, Su W, Liu Z, Zhou M, Chen S, Chen Y, Lu D, Liu Y, Fan Y, Zheng Y, Han Z, Kong D, Wu JC, Xiang R, Li Z (2012) CD44 antibody-targeted liposomal nanoparticles for molecular imaging and therapy of hepatocellular carcinoma. Biomaterials 33(20): 5107–5114. doi:10.1016/j.biomaterials. 2012.03.067
Su W, Zhou M, Zheng Y, Fan Y, Wang L, Han Z, Kong D, Zhao RC, Wu JC, Xiang R, Li Z (2011) Bioluminescence reporter gene imaging characterize human embryonic stem cell-derived teratoma formation. J Cell Biochem 112(3):840–848. doi:10.1002/jcb.22982
Li Z, Suzuki Y, Huang M, Cao F, Xie X, Connolly AJ, Yang PC, Wu JC (2008) Comparison of reporter gene and iron particle labeling for tracking fate of human embryonic stem cells and differentiated endothelial cells in living subjects. Stem Cells 26(4):864–873. doi:10.1634/stemcells.2007-0843
Acknowledgments
This work was partially supported by grants from the National Basic Research Program of China (2011CB964903), National Natural Science Foundation of China (31071308), Tianjin Natural Science Foundation (12JCZDJC24900), NCET of State Education Ministry (NCET-12-0282) and Fundamental Research Funds for the Central Universities (65121018).
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Su, W., Leng, L., Han, Z., He, Z., Li, Z. (2013). Bioluminescence Imaging of Human Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Myocardial Infarction. In: Turksen, K. (eds) Imaging and Tracking Stem Cells. Methods in Molecular Biology, vol 1052. Humana Press, Totowa, NJ. https://doi.org/10.1007/7651_2013_15
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DOI: https://doi.org/10.1007/7651_2013_15
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