Abstract
The consumption of contaminated vegetables has a great impact on human health. Due to this fact, we conduct the study to estimate the heavy metals in groundwater, soil, and vegetables by using the atomic adsorption spectroscopy (AAS) and find out the health risk using THQ and TCR caused by using these vegetables. The mean concentrations of As (0.015–0.40 mg/L), Cd (0.02–0.029 mg/L), Co (0.31–0.38 mg/L), Cr (1.02–1.09 mg/L), Cu (2.14–2.17 mg/L), and Hg (0.01–0.04 mg/L) are high in groundwater from threshold values given by WHO. The mean concentrations of As (22.17–23.14 mg/kg), Cd (4.21–4.54 mg/kg), Cu (21.24–24.36 mg/kg), and Pb (32.12–33.48 mg/kg) are high in soil samples from threshold values given by WHO. The mean concentrations of As, Cd, Pb, Cr, Fe, Hg, and Mn values exceeded the recommended values with concentration ranges: 1.75–4.56, 0.41–0.67, 2.12–3.12, 1.44–4.56, 87.12–135.25, 2.09–2.64, and 33.41–129.32 mg/Kg, respectively. The vegetable sample’s average concentration of heavy metals was in decreasing order cabbage ˃ brinjal ˃ okra ˃ tomato. The EDI values for As, Co, and Hg calculated for both adults and children is high. The target hazard quotients (THQ) for As, Co, and Hg are greater than the threshold value by consuming vegetables, which indicated the health risk for both adults and children. Similarly, HI due to tomato, cabbage, okra, and brinjal’s consumption is ˃ 1, with HI values 8.1975, 15.3077, 8.7312, and 10.2306, respectively. This advised the possible health effect in this area by using these vegetables. Target Cancer risk (TCR) exposed the adverse cancer risk persuaded by As, Cr, and Hg as their values exceeded the normal range by USEPA by consumption of these vegetables. This study concluded that vegetables imply the total health risk on local people, and regular monitoring of heavy metals is strongly suggested in this region.
Article Highlights
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Heavy metals distribution in this study area is under the impact of urbanization, industrialized and agricultural activities.
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BCF showed the transfer of Fe and Hg from soil to edible parts of vegetables.
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TCR for As, Cd, Cr, Ni, and Pb shows the toxicological risk in this region.
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1 Introduction
Heavy metals influence our environment as they are primary polluting agents in our food source, specifically vegetables [1]. The discharge of industrial waste has the potential of polluting water. This polluted water directly affects soil, agricultural fields, and rivers, which cause to produce multiple sources of pollution [2, 3]. Heavy metals flow in soil and water, which is deeply concerned about public health, farming production, and ecological strength [4,5,6,7]. Pakistan faces a deficiency of external water resources, so their agricultural activities rely on wastewater usage in city and peri-urban areas [8]. Human activities like industrial waste, transportation, and agriculture emit a large concentration of heavy metal elements on the soil surface and groundwater [9]. Heavy metals such as Cd, Ni, and Pb are not required to grow plants. These metals mainly accumulate in plants in hazardous forms, which can cause dangerous diseases in humans and wildlife [10]. These heavy metals are categories as essential and toxic. Fe, Cu, Zn, Co, Mn, Mo are necessary for plant’s growth in adequate amount, whereas Hg, Cd, Pb, Sn, Cr, As are known as toxic metals. Toxic heavy metals also affect plants’ growth and cause structural damage and deformation in their physiological and biochemical activities. Heavy metal absorptions in vegetables and nutrition have contrary relationships that influence the various portions of vegetables (protein, fat, and carbohydrate) [11, 12]. Many studies have been conducted on heavy metal pollution in developed countries' food chains [13,14,15,16].
However, some studies have been conducted in developing regions [17,18,19,20]. In Pakistan, published data on heavy metal contamination in the food chain are not substantial, a few reports for references [21,22,23,24,25,26,27]. In the farmland in northern Lahore, industrial wastewater and municipal wastewater are usually used to grow vegetables. These vegetables are widely used in urban and rural areas. The purpose of this study was to ensure that the heavy metals added to the effluents seep down to the groundwater and then cause various diseases in human beings living in nearby areas of Kasur, Pakistan. This study comprises of estimating the concentration of selected heavy metals (Fe, Hg, Co, Mn, Pb, Zn, Cr, Ni, Cu, As, and Cd) in water, soil, and vegetable’s samples near the selected area and finding out the impact of usage of water and vegetables on local people. Some factors such as BCF (bioaccumulation factor), EDI (estimate daily intake), THQ (target hazard quotient), HI (health index), and TCR (target cancer risk) (for As, Cd, Cr, Ni, and Pb) were calculated to find out the health risk for human beings living in this area.
2 Material and methods
2.1 The geographical location of the study area
Kasur (Romanized as Qasūr, meaning palace) has a latitude 31o 70 N and longitude 74o 27′0″ E and is located in the east of Punjab Province, Pakistan–Indian border. The region encompassed by the research area mostly includes small villages and agricultural land irrigated by the river water or groundwater [28]. Significant crops cultivated in this area are sugar cane, maize, wheat, and some vegetables (brinjal, okra, tomatoes, cauliflower, cabbage, and reddish). Kasur city has a traditional industrial setup with many small tannery units existing in the residential areas [28]. Kasur city, rich in the tanning industry, occupies 50% of Pakistan’s entire tanning industry. Out of these existing tanning units, about 90% of the tanneries have adopted the chrome tanning process [29]. Several possible scattered sources in the tannery area's vicinity may cause subsurface contamination. Therefore, it is impossible to define a single-point source causing this contamination. Drain Rohi is not lined when it comes out of the city. It is also carrying industrial effluent and municipal wastewater, so there is a great possibility of percolating hazardous chemicals into the soil. The above-stated facts mean that the effluent has seeped into the ground. The drains could be line sources of contamination and are causing the area of contamination to expand farther from the tannery area. Due to the relatively high permeability of soil underlying the drain, the groundwater is rapidly contaminated by the hazardous chemicals originating from the tannery effluent [28]. The soils are reddish brown to grayish brown, mostly medium-coarse and medium-textured soils, containing a high percentage of fine to very fine sand and silty clay. The clay part of the soil consists of nonswelling materials, i.e., silty clay loam. The entire area is underlined mostly by sand at various depths, below the soil for the drain [30]. Figure 1 shows the map of study area along with sampling sites.
2.2 Groundwater, soil, and vegetable sampling
Forty-eight groundwater samples were collected in triplicate, used for watering the research field, and located near the drainage sites. Forty-eight soil samples from four different areas (12 samples from each field) were collected by digging up a monolith (30 × 30 × 30 cm3). Two kilograms of soil was collected in triplicate from each field stored in a polyethylene bag and sent to the chemistry laboratory for further analysis. Soil samples were air-dried, crushed, and passed out by a 2 mm sieve and stored at room temperature. One kilogram of edible parts of each vegetable was collected in triplicate from each site and stored in a polyethylene bag. After this, each vegetable is washed out with tap water and then washed three times with deionized water to remove the surface pollutants and other impurities. Then, the vegetables were sliced into small pieces and kept air-dried for two hours. After the air-drying, these samples were poured into a silica plate and kept in an oven at 110 °C for drying. The dried sample was ground into the mortar until it could pass out from a 2 mm sieve and then stored in desiccators before putting into a muffle furnace to convert into ash for 12 h at 500 °C [31].
2.3 Physiochemical properties of samples
Groundwater and soil pH were calculated by pH meter by Maclean [32]. The sample's conductivity was calculated by an electrical conductivity meter Cyberscan Pc 510 (Eutech instrument). For BOD Jenway 970 (Cole-Parmer Instrument), HACH methods are used (8043). For COD (Lovibond RD 125 m), HACH method was used (8000). Total nitrogen and total phosphate of soil were determined by Bremner and Mulvaney [33].
2.4 Samples digestion for determination of heavy metals
Large particles and wreckages from the soil sample were removed and dried at 110 °C in the oven. One gram of each sample was placed in a flask, and 15 mL acid mixture of 5:1:1 (70% HNO3, 70% H2SO4 and 65% HCLO4) was added and kept in the mechanical shaker at 80 °C until clear solution obtained. After cooling, the solution was filtered with Whatman no 42 filter paper, then poured into a 50-ml volumetric flask, and diluted until the mark for further analysis. Samples were analyzed for different heavy metals such as Cd, Ni, Cu, Zn, Fe, Pb, and Cr with the atomic absorption spectrometer’s help (Model GBC-932 plus U.K). Analysis using AAS was carried out at the most analytical spectral lines of the metals (Zn 228.8 nm, Pb 217 nm, Ni 232 nm, Cu 324.8 nm, Cr 357.9 nm, Fe 309 nm, Cd 248.3 nm, and As 193.7 nm) [34,35,36]. For As determination, the slotted tube atom trap with an inert gas/hydrogen was held in the flame by a simple holder. The burner clips were used to enhance the flame sensitivity and improve the detection limit for As. Ten milliliters of water sample was digested with 2 ml HNO3 and 5 ml HCl and heated at 95 °C until the transparent solution was obtained, and then the solution was filtered and cooled. Total volume was adjusted at 50 ml, and the heavy metals were estimated by AAS [34].
2.5 Bioconcentration factor
The bioconcentration factor was calculated as given in Eq. (1):
Cplant shows the metal concentration in edible parts of vegetables, and Csoil shows the metal concentration in soil. If BCF is greater than one, then the plant is a potential accumulator of heavy metal and should be analyzed.
2.6 Health hazard factors
2.6.1 Estimated daily intake (EDI)
The daily intake of vegetables is calculated from Eq. (2):
where Cm represents the metal concentration (mg/kg of dry weight), Ef represents the exposure frequency (365d/a), De represents the exposure duration (70), Tav represents the average time of exposure (365 days × 70). FIR represents the average food consumption (300–350 g/person/day) given by WHO [37]. In this study, we used mean of this consumption 325/115 g/person/day, Cf represents the conversion factor of vegetable into dry weight (0.085) [38] and Wb represents the average body weight of consumer (70/15 kg) (FAO/WHO) [39].
2.6.2 Target hazard quotient (THQ)
The target hazard quotient is calculated using Eq. (3) [33]:
EDI is estimated daily intake and Df represents the reference dose. If the THQ value is greater than one, then there is a chance for a noncarcinogenic effect related to values. If THQ is less than one, it will be assumed to be safe for noncarcinogenic effects [40]. Overall data for find out the THQ are compiled in Table 1.
2.6.3 Hazard index (HI)
It is calculated by the given formula in Eq. (4) [41, 42]:
where CMn represents the concentration of manganese and DfMn represents the oral reference dose of manganese. If HI is greater than one, then there is a health significance resulting from exposure to a particular element. This HI value greater than one is not acceptable. HI values are classified into negligible, low risk, medium risk, and high risk [43].
2.6.4 Cancer risk
It can be calculated by using Eq. (6)
where EDI represents the estimated daily intake and CPSo represents the oral cancer slope factor. Some CPSo values for As, Pb, Ni, Cr, and Cd are given in Table 1.
2.7 Statistical analysis of heavy metals
Statistical analysis was done using Origin 2018 to evaluate the significant differences between heavy metals concentration in the vegetables, soil, and water samples. Principle component analysis (PCA) was used to determine the potential heavy metal source in water, soil, and vegetable samples.
3 Results and discussion
3.1 Physiochemical properties and heavy metals in groundwater
Heavy metals contaminate groundwater used for drinking purposes and cultivation process through chemical use for agricultural, municipal waste, and industrial effluents. The physiochemical parameter of groundwater is summarized in Table 2, which shows that pH, ECs, BOD, COD, and TDS of groundwater are in a permissible range given by the WHO [37]. This region’s mean pH values range from 7.93 to 8.17, which lie within the productive water range of 6–9 [44]. Some of these heavy metals in groundwater are good for human health in trace amounts, but they produce water pollution and become threat for human health when their amount exceeded. The heavy metals in groundwater exceeded the permissible limit set by WHO [37]. The heavy metal concentration in groundwater is shown in Table 2, and the highest concentration calculated for As (0.04 ± 0.4 mg/L), Cd (0.03 ± 0.4 mg/L), Co (0.38 ± 0.8 mg/L), Cr (1.09 ± 0.2 mg/L), Cu (2.17 ± 1.7 mg/L), and Hg (0.04 ± 0.001 mg/L) exceeded the permissible limit set by WHO [37]. While the highest concentration for Fe (0.54 ± 0.1 mg/L), Mn (0.31 ± 0.4 mg/L), Ni (0.02 ± 0.01 mg/L), Pb (0.02 ± 0.01 mg/L), and Zn (3.4 ± 1.4 mg/L) was found in the permissible range. In recent studies, the allowable amount of groundwater in various Pakistan regions has far exceeded the limit. The previous study found that the amount of metals Cd (0.04), Co (0.15), Cr (1.32), Fe (0.56), Mn (0.07), Ni (0.11), Pb (0.14), and Zn (0.14) (mg/L) is mainly used for groundwater near the Kasur Industrial Zone in Pakistan, which is many times higher than the WHO standard [30]. ULLAH [45] found the high values of Mn (0.03), Zn (0.16), Pb (0.49), Fe (0.30), Cu (0.06), Ni (0.10) and Cr (0.03) in groundwater of Sialkot, Pakistan. Besides, Haq [46] also reported the Pb concentration (0.146 mg/L) in groundwater in 18 areas of Karachi, which exceeded the allowable limit (0.05 mg/L) set by the WHO.
3.2 Physiochemical properties of soil
Physiochemical properties analyzed for the soil under the different vegetables used in this study are given in Table 3. The analyzed soil pH varies from 8.09 to 8.34, which revealed that the study area is slightly alkaline [16]. The electrical conductivity (ECs) values vary from 148 to 169 µS/cm, which means that soil has a balanced nutrient amount for plant growth [16]. The soil’s total nitrogen content ranges from 0.048 to 0.057, which reveals that groundwater has less nitrogen content. Total phosphate values in the analyzed soil vary from 0.058 to 0.071. Lower phosphate values in this soil showed that soil’s pH plays a vital role in the bioavailability of phosphorous plants [47]. The moisture content of the soil samples analyzed under this study varies from 24.8 to 27.4%.
3.3 Level of heavy metals in soil and vegetables
3.3.1 Heavy metals in soil samples
The heavy metal concentration in soil from the study area has been assessed. The obtained data are presented in Table 4, which demonstrates that all soil samples are positive for heavy metals. The result revealed that As concentration ranged from 22.39 to 23.14 mg/kg, which was higher than the permissible range given by US-EPA (14 mg/kg) for agricultural soil. The high concentration of As indicated that metal and its compound are released from industries situated near this area [48]. Cd concentrations ranging from 4.21 to 4.54 mg/kg have been found higher than US-EPA’s normal values (0.3 mg/kg). The Co (7.31–7.56 mg/kg) and Cr (23.25–25.64 mg/kg) concentration ranges in this study have been found in the permissible range. The concentration range of Cu (21.32–24.36 mg/kg) is slightly high in all soil samples from their permissible range. Previous study values of Cd (2–3.4 mg/kg), Cr (54.1–210.2 mg/kg), and Cu (31.2–60.8 mg/kg) reported in this Kasur area resemble those of the current study [49]. The mean concentration of Fe (51.32–56.28 mg/kg) in cultivation soil was found low for elevated iron as described by a previous study[50] but somehow related to the study done by Rattan [51]. The concentration ranges of Hg (0.21–0.27 mg/kg), Mn (1248–1341 mg/kg), and Ni (21.36–21.94 mg/kg) in this cultivation soil were found within the permissible range. The concentration ranges of Pb(32.12–33.48 mg/kg) and Zn (91.36–94.56 mg/kg) were found higher than the permissible range of all soil samples. The Pb and Zn concentration corresponds with the previous study values (23–35 mg/kg and 55.13–95.23 mg/kg) conducted in this area [8]. In general, this study’s data revealed that the cultivation soil under all vegetables is contaminated with a high concentration of As, Cd, Cu, Pb, and Zn. Their limits have exceeded their normal range given by the WHO [37].
3.3.2 Heavy metals in vegetables
In current ages, food hygiene and safety have become an important challenge in emerging countries due to improper management of wastage released from industries. Thus, the heavy metal concentration in commonly consumed vegetables was analyzed to ensure food safety and quality in this study.
The data of heavy metal concentration for all cultivated vegetables in this study are shown in Table 5. The mean concentration of As (1.75–4.46 mg/kg) is higher than the normal values given by FAO/WHO [39]. The previous study conducted in Bangladesh shows the mean concentration of As in leafy and fruity vegetables is 0.28 mg/kg (0.09–0.43 mg/kg) and 2.24 mg/kg (0.009–7.9 mg/kg), respectively [52, 53]. These result revealed that As concentration is high than the previous study conduct in this region, which is due to the usage of As-enriched fertilizer to cultivate vegetables [54]. Cd’s mean concentration ranged 0.41–0.67 mg/kg in all vegetables collected from the study area. While Cd concentration values in previous study conduct in Pakistan are (0.02–0.08 mg/kg), (0.01–0.69 mg/kg), (0.093–4.09 mg/kg) [55–57,56,], and study conduct in Bangladesh is (0.001–2.2 mg/kg) [58], respectively. The Cd level in all vegetables used in this study is higher than the normal values given by FAO [39]. This is due to the extreme usage of inorganic fertilizer, wastewater irrigation, and metal emission from industries.
Co’s mean concentration varied from 0.57 to 1.36 mg/kg in all vegetable samples collected from the study area. Compared with previous studies conducted in Pakistan, the current study result varied from 1.08 to 13.6 mg/kg in different vegetables that fall within the permissible range [27]. The mean concentration of Cr ranged from 1.44 to 4.56 mg/kg in vegetable samples. The Cr concentration in a previous study (0.2–3.98 mg/kg) done in Pakistan [27] resembles this study, but Cr concentration is lower than the previous study done in Bangladesh (2.1–33.16 mg/kg) [19]. The mean concentration of Fe (87.12–135.25 mg/kg), Hg (2.09–2.65 mg/kg), Mn (33.41–129.32 mg/kg) is found high in all vegetables also from the previous study conducted in Pakistan [59] and Bangladesh [57]. Fe metal is used in photosynthesis and chlorophyll synthesis. That is why all vegetables contain more amount of iron. Ni’s mean concentration ranged from 1.36 to 3.12 mg/kg, which resembled the values obtained with the previous study, whose mean concentration varied from 1.41 to 37.52 mg/kg [19]. The mean concentration of Pb in all vegetable samples varied from 2.12 to 3.21 mg/kg, which is much higher than the previous study done in this region [27, 59]. The Pb concentration resembled the values obtained from the study conducted in India and Bangladesh [19, 57, 58]. Pb’s high value in all vegetable samples indicated that these fields were irrigated with the untreated wastewater discharge from paint and Pb smelting industries in this region [19]. The mean concentration of Zn varies from 20.36 to 22.67 mg/kg. Zn’s value in all vegetable samples is higher than the previous study conducted in Pakistan [55]. In contrast, the Zn concentration coincides with the study conduct in Bangladesh [57].
It is observed from Table 5 that the heavy metals accumulation in the leafy vegetable is higher than the fruity vegetables because leafy vegetables have a high transpiration rate to sustain the plant growth and moisture content in vegetables [19, 27]. Also, heavy metal concentration in vegetables highly depends on environmental and geological location. The current study results were compared with previous studies, and the data are given in Table 6.
3.4 Source analysis of heavy metals in vegetables
Pearson’s correlation coefficient matrix method was used to determine the interrelationship between the vegetable sample’s heavy metals, as shown in Table 7. The inter-metal interface shows the causes and pathways of heavy metals in vegetable samples. As shows the strong positive relationship with the Cd (0.939), Co (0.956), Cr (0.987), Fe (0.996), Hg (0.951), and Mn (0.977), while a moderately strong relationship with Ni (0.646) and Pb (0.628) and strong negative relationship with Cu (− 0.790) and Zn (− 0.916). Cd, Co, Cr, Cu Fe, Hg, and Ni show a strong correlation. In contrast, Pb and Zn do not offer any significant strong and negative correlation with other metals. The high correlation coefficient between metals shows the common source, interdependence, nearly or similar metal accumulation in vegetable samples [17].
To determine the hypothetical source of heavy metals (natural or human) in vegetable samples, principal component analysis (PC) was performed according to the standard procedure described in the literature [60, 61]. The PCA was completed in a tabular and dimensionless standardized form of the dataset and is presented in Table 8 and Fig. 2. Two principal components (PC) were obtained, and their variances were explained by 84.996% and 9.129% for this study. Overall, PCA reveals two significant categories of 11 heavy metals studied in vegetables. PC1 is highly synchronized with As, Cd, Co, Cr, Fe, Hg, Ni, Mn, and Pb, while PC2 is highly synchronized with Cu and Zn. The sources of PC1 and PC2 can be considered as mixed sources of human input, especially industrial pollution and agricultural activities in the study area. Vehicle emissions and environmental reserves were released by burning coal and fuel, and it is believed that vegetable samples have been collected in these metals in urban areas. Because heavy metals are released into the atmosphere and accumulated by plants, PCA analysis shows that the intensity of similar heavy metals in vegetables is not the same.
3.5 Bioconcentration factor (BCF)
The deposition and transfer route of heavy metals from soil to the edible part of vegetables is the main entry of heavy metals in food [58]. We have calculated heavy metal transferability of soil to plant for vegetables used in this study which is shown in Table 9. BCF factor for heavy metals As, Co, Cd, Cr, Cu, Mn, Ni, Pb, and Zn is less than one, which indicates that these metals are not accumulating in the plant through soil. While the BCF for Fe accumulating in tomato is (1.55), Brinjal (1.88), cabbage (2.49), and okra (1.84) and BCF for Hg accumulating in tomato (9.35), Brinjal (11.00), cabbage (9.78), and okra (8.71). BCF for each vegetable was subjected to a statistical test (one-way ANOVA test) to estimate the existence or absence of numerical difference between these vegetables and result revealed that the 95% significantly different expect Cd, Zn, and Pb [42].
3.6 Health risk assessment
US-EPA has introduced health risk (carcinogenic and noncarcinogenic health risk) parameters to determine the health risk due to the exposure of toxic metals when used for a long time [50].
3.6.1 Estimated daily intake
EDI refers to the estimated daily intake. The calculation is based on each metal’s mean concentration in food and respective consumption of rate and done by Eq. (2), and data are displayed in Table 10. By comparing these values with the reference value, we determined that EDI values for heavy metals As, Co, and Hg were high and calculated for both adults and children. The NYSDOH (New York State Department of Health) suggests if EDI/Df ratio is ≤ Df, it is related to minimum health risk. If it is 1–5 times higher than Df,, it is related to low health risk. If it is 5–10 times higher than Df, it is related to moderate health risk, and if it is ten times higher than Df, it is related to high health risk [50]. From EDI/Df ratio, we concluded that the As metal in fruity vegetables is 1–5 higher than Df, so low health risk for using these vegetables grown in this area while in leafy vegetables, it is 5–10 times high so moderate health risk for using this vegetable.
3.6.2 Target hazard quotient
THQ is related to the noncarcinogenic health risk, and its acceptable value is ≤ 1 [51]. Ambedkar and Maniyan (2011) resolved that if THQ values exceed their limit, it will be associated with health risk. It will be calculated by Eq. (3), and its data are shown in Table 11. In this study, the THQ values for As, Co, and Hg are greater than 1 in all vegetables. So, their THQ values could carry the noncarcinogenic risk in this area population. From these values, we observed that the leafy vegetables’ THQ values are higher than the fruity vegetables except Cu and Zn.
3.6.3 Hazard index
HI represents the cumulative effect of the ingestion of toxic metals from the usage of contaminated vegetables, and data of HI shown in Table 11 indicate that HI values are higher than the permissible limit (1) for all vegetables. So, this study area's vegetable intake will be linked with noncarcinogenic health risk [42].
It is an allusion here that this present study assessed the EDI, THQ, and HI values which were based on the estimated daily vegetable consumption, which was about 325 g per day for both study vegetables. So, it is probable that EDI and THQ values might be overvalued and might impact on HI values as well. Meanwhile, it should be noted that the present study had only considered cabbage, brinjal, okra, and tomato to estimate possible noncarcinogenic and carcinogenic health risks of the population in Kasur and its surrounding. Hence, this study’s result took into account part but not the real threat to the people in the study area. As a result, the local population’s potential health risks due to the exposure to heavy metals through the consumption of vegetables might be underestimated.
3.6.4 Target cancer risk
Overall, it is supposed that when people interact with toxic metals, it may produce the bad effect on their health, and prolonged contact with specific carcinogenic metal may lead to cancer, and heal risk increases with time. TCR signifies the approximation of the predictable cancers. Then, it also signifies the opportunity to evolve cancer-causing risks in an individual. TCR is due to the exposure of toxic metals such as As, Cd, Cr, Ni, and Pb by consuming contaminated vegetables calculated by EDI and CPSo values and obtained result shown in Table 12. NYSDOH stated that If TCR values are ≤ 10–6, then it relates to low cancer-causing risks, if its values lie between 10–5 and 10–4, then it relates to moderate cancer-causing risks, and if values lie between 10–3 and 10–1, then it relates to high stakes [50].
TCR values for As and Ni for all vegetables stay in the 10–3–10–1, indicating the high carcinogenic risk. While the TCR value of Cr in cabbage also remains 10–3–10–1, it also shows the high risk. All other TCR values for all metals are within the 10–5–10–4 range, so these relate to the moderate cancer risk.
4 Conclusion
This study indicated that heavy metal concentration in groundwater is high from FAO’s standard range. The mean concentration of As, Cd, Cu, Pb, and Zn in soil samples is higher than the permissible range. Similarly, heavy metals such as As, Cd, Pb, Cr, Fe, Hg, and Mn values exceeded the recommended values set by FAO/WHO in all vegetable samples. From human health point of view, THQ values of As (2.67), Co (1.01), and Hg (3.30) by consumption of tomato are ˃ 1. Similarly, THQ values of As (4.07), Co (1.29), and Hg (3.55) by consumption of brinjal are ˃ 1. The GTHQ by consumption of all vegetables was˃ 1 for As (17.30), Co (5.26), Cr (1.52), Hg (14.11), and Pb (1.41). Health index (HI) for tomato (8.19), brinjal (10.23), cabbage (15.31), and okra (8.73) is found ˃ 1. TCR values for As and Ni for all vegetables stay in the 10–3–10–1, indicating the high carcinogenic risk. While the TCR value of Cr in cabbage also remains in 10–3–10–1, it also shows the high risk. All other TCR values for all metals are within the 10–5–10–4 range, so these relate to the moderate cancer risk. The total health risk showed the ingestion of vegetable presence health risk in the Kasur area and its surroundings.
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IA and FA conceived the concept. IA and AS designed the experimental work. IA, FA, AS, ARA, and TP collected and analyzed the data. IA prepared the manuscript. ARA and TP proofread the document and correction. All authors approved the final version of the manuscript.
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Ashraf, I., Ahmad, F., Sharif, A. et al. Heavy metals assessment in water, soil, vegetables and their associated health risks via consumption of vegetables, District Kasur, Pakistan. SN Appl. Sci. 3, 552 (2021). https://doi.org/10.1007/s42452-021-04547-y
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DOI: https://doi.org/10.1007/s42452-021-04547-y