Abstract
Yerba mate is a tree natural from South America, traditionally daily consumed as hot (chimarrão) or cold (tereré) and roasted leaves (mate tea) infusions, being an important source of polyphenols and flavonoids in human nutrition. To produce bioactive-rich extracts from yerba mate, oven-dried leaves at 35 °C and three different commercial products, named M, T, and S, were extracted using different General Recognized as Safe solvents (distilled water (W), ethanol (E), and water: ethanol (1:1, v:v) (WE)). The yerba mate extracts were analyzed for total and soluble solids, titratable acidity, total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity. The WE solvent resulted in the highest TPC and TFC. However, the commercial samples showed a higher concentration of phytochemicals than oven-dried leaves, and the commercial sample T showed the lowest content of total solids and the highest content of soluble solids (°Brix). The industrial processing of yerba mate aids the TPC and TFC preservation, resulting in extracts with superior phytochemicals concentration when compared with the oven-dried sample.
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
1 Introduction
Yerba mate or maté (Ilex paraguariensis A.St.-Hil.) is a native tree from South America and it has a natural distribution area of 540,000 km2 between northwest Argentina, eastern Paraguay, and southern Brazil [2]. In these regions, it has great environmental and socio-economic importance, creating jobs and increasing incomes, as it is the main non-timber forest product [22]. The parts of the plant used are the leaves and variable fractions of thin branches. Traditional consumption is carried out by locals as a hot infusion (chimarrão), cold infusion (tereré), or even as hot or cold roasted leaves infusions (mate tea). Many consumers drink 1–2 L/day of yerba mate infusions, which correspond to about 12–23 g/habitants/day [4, 7]. This amount is equivalent to the consumption of tea (Camellia sinensis) in Asia and Europe, and coffee in Europe and North America [4].
The yerba mate bioactive compounds with functional properties (about 10% in dry weight) are particularly polyphenols, chlorogenic acids (caffeoylquinic (CQA), and dicaffeoylquinic acids (DQA)) and caffeic acid; alkaloids, caffeine, and theophylline; flavonoids quercetin, rutin, lutein, and myricetin; and terpenoids ursolic and oleanolic acids [2, 16, 17]. These phytochemicals are known for their anti-inflammatory, anti-obesogenic, antimutagenic, antibacterial, and antiviral capacity, as well as their antioxidant capacity [1, 3, 4, 8, 9, 12, 20].
The main steps for chimarrão and tereré mate industrial processing are the sapeco (thermal bleaching using flames), flash drying at high temperatures (300–400°C), milling, sieving, and particle size standardization [13, 18]. The mate phytochemical extraction and purification also have high technical and economic potential. However, the extraction procedure and solvent type impact the phytochemicals recovery, being preferred General Recognized as Safe (GRAS) and environmentally friendly solvents.
We hypothesize that industrial processing decreases the concentration of the phytochemical compounds in fluid yerba mate extracts. For this reason, the aim was to produce bioactive-rich extracts from oven-dried leaves and different commercial chimarrão-type yerba mate with different GRAS solvents.
2 Material and methods
Between March and May 2017, in natura leaves of yerba mate were collected from Laranjeiras do Sul, Paraná State, Brazil (25° 24′ 28" S 52° 24′ 57" O). The samples were from 10-year-old trees, agroforestry shaded, with random and stratified sampling in all parts of the plant. All leaves were oven-dried at 35ºC for 72 h, followed by knife milling (Fortinox, Star FT-50, Brazil). Three commercial yerba mate brands (M, T, and S) of native leaves for chimarrão (no added sugar), manufactured in March and April of 2017, non-vacuum packed, were purchased from groceries of the same city. Each sample was sieved at 20 mesh to remove small and thin branches and stems and vacuum stored at -20 °C.
All samples were extracted (8 g in 40 mL of solvent) by distilled water (W), anhydrous ethanol (E), and water: ethanol 1:1 (v:v) (WE), after 5 h of water bath Dubnoff incubation (50 °C) [14]. The extracts were evaluated for soluble and total solids, and total titratable acidity [23]. The total phenolic content (TPC) was analyzed using Folin Ciocalteu reagent, and the results were express as gallic acid (G7384, Sigma-Aldrich San Luis, Missouri, EUA) equivalent (GAE) [5]. The total flavonoid (TFC) content was investigated by aluminum-flavonoid complexation, and the results were express as catechin (C1251, Sigma-Aldrich San Luis, Missouri, EUA) equivalent [10]. Total antioxidant capacity was determined by the scavenging of free radicals DPPH (2,2-Diphenyl-1-picrylhydrazyl, D9132, Sigma-Aldrich San Luis, Missouri, EUA), and ABTS (2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), A1888, Sigma-Aldrich San Luis, Missouri, EUA), and the results were express as Trolox® ((±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid, 238,813, Sigma-Aldrich San Luis, Missouri, EUA) equivalent. All results were expressed in dry mass of yerba mate and statistically evaluated by analysis of variance and Duncan's test mean comparison (p > 0.05) (SAS® Institute Inc, Cary, USA).
3 Results and discussion
The different solvents influenced the total solids of the fluid extracts (Table 1). Ethanol provided the highest of extracted total solids, followed by WE (p < 0.05). The concentration of total solids can reflect the yield of the extraction process, being influenced by the solubility of solids in solvents, sample particle size, and the separation or filtration of the solid particles. Therefore, smaller particles tend to favor extractive processes, as they have a big contact surface area, and also make it difficult to remove them by filtration processes, resulting in high total solids concentrations [1].
Commercial products resulted in the highest soluble solids (p < 0.05), mainly for water extraction (Table 1). The quantification of soluble solids is an easy and fast tool to estimate the content of sugar, organic acids, and small constituents in food products [21]. The use of ethanol, even if diluted (1:1), favors the extraction of sugar, organic acids, and other components, with a polarity similar to that of the solvent, present in yerba mate samples. The lowest acidity was observed in the ethanol extracts, except for the commercial sample S, showing that extraction of acids was hampered using ethanol (Table 1).
The TPC extraction using WE was more efficient than the solvents W and E, especially in the commercial yerba mate (Table 1). Only in the oven-dried sample (F), the solvent did not experience effects in the polyphenols extraction (p > 0.05) (Table 1). Regarding the type of yerba mate, it was observed that the content of phenolic compounds was related to the yerba mate processing (Table 2). The highest TPC was observed in commercial yerba mate band M (65.78 ± 1.54 mg gallic acid g−1) using WE solvent. Sample F resulted in the lowest TPC extraction, using WE as the solvent. The oven-dried sample was not bleached, and enzymes could continue active during mild temperature oven-drying and milling steps. The temperature that was used (35 °C) was not enough to inactivate the polyphenol oxidase and peroxidase enzymes which naturally occur in yerba mate leaves [7, 18], resulting in products with lower phytochemical compounds.
Phenolic compounds feature a variety of chemical structures, ranging from simple molecules to high degree polymerized molecules [19]. Based on this, the differences in the concentration of total phenolic compounds in the aqueous, alcoholic, and hydroalcoholic extracts (Table 2) were consistent, since hydroalcoholic solvents are considered more useful in the extraction of this class of compounds.
The leaves, branches, and bark from I. paraguariensis have different phenolic compound classes and concentrations [20]. Total phenolic compounds and antioxidant activity, by DPPH scavenging, were high in methanolic and aqueous extracts from bark and branches than from leaves samples [20]. Commercial products did not inform in the labels which concentration of the tree part (leaves and branches) composed the product consequently, showing a natural variation of color, flavor, and phytochemical composition. Besides, during the milling process, larger branches can be re-milled to the standardization of commercial products. Therefore, the sample F, composed exclusively of leaves, should consequently present higher antioxidant activity. However, this sample showed the highest antioxidant activity (Table 2). This result corroborates TPC and TFC, suggesting that enzymatic degradation promoted greater influence than thermal degradation at yerba mate compounds. It results in products with relatively low antioxidant activity and, ethanolic extracts tended to show higher antioxidant activity than water extracts.
The WE extracts showed high total flavonoids concentration, except for the F yerba mate sample (p < 0.05) (Table 2). The highest flavonoid content was observed in commercial T (5357 ± 373 mg catechin g−1) using WE solvent. It has been reported high flavonoids content using WE compared to W when analyzing extracts from yerba mate residues [11]. The results can be due to the high rutin extracting [13], and low quercetin solubility in water [6]. Both groups, phenolic and flavonoids compounds, have structures that are characterized by their high antioxidant potential [3].
Thereby, the yerba mate extracts stand out because of their high antioxidant capacity compared to other plants and tea herbs [16]; and the ethanolic extracts tend to show higher antioxidant activity than water extracts. The WE solvent (1:1, v:v) resulted in extracts with a higher concentration of total phenolic compounds and flavonoids than W or E, which correlate with antioxidant activity [15].
4 Conclusion
The GRAS solvent WE resulted in mate extracts with high total phenolic and flavonoid compounds, which corroborate with antioxidant activity. Oven-dried leaves, non-thermal bleached, showed the lowest phytochemical compound concentration on fluid extracts. The commercial yerba mate T presented the lowest content of total solids and the highest content of soluble solids (°Brix) among all brands, indicating good extraction efficiency of WE solvent. Oppositely to our initial hypothesis, the industrial yerba mate processing for commercial products resulted in superior yield extraction and preserves the phenolic and flavonoids compounds, compared to the mild drying process of mate leaves. The WE solvent and commercial yerba mate samples can be used for extract production, being safe and useful for drugs or food production and manufacturing.
Data Availability
The supporting data are available from the corresponding author upon reasonable request.
References
Bastos DHM, Saldanha LA, Catharino RR et al (2007) Phenolic antioxidants identified by ESI-MS from yerba maté (Ilex paraguariensis) and green tea (Camelia sinensis) extracts. Molecules 12:423–432. https://doi.org/10.3390/12030423
Berté K, Rucker N, Hoffmann-Ribani R (2011) Yerba maté Ilex paraguariensis A.St.-Hil. Phytotherapie 9:180–184. https://doi.org/10.1007/s10298-011-0629-2
Bisognin DA, da Luz LV, Lencina KH et al (2019) Contents of total phenolics and flavonoids in and antioxidant activity of Ilex paraguariensis leaves. Pesqui Agropecuária Bras 54:1–8. https://doi.org/10.1590/s1678-3921.pab2019.v54.00856
Bracesco N, Sanchez AG, Contreras V et al (2011) Recent advances on Ilex paraguariensis research : Minireview. J Ethnopharmacol 136:378–384. https://doi.org/10.1016/j.jep.2010.06.032
Bucić-Kojić A, Planinić M, Tomas S et al (2007) Study of solid-liquid extraction kinetics of total polyphenols from grape seeds. J Food Eng 81:236–242. https://doi.org/10.1016/j.jfoodeng.2006.10.027
Carvalho M, Santos Â, Ghassan L et al (2017) The effects of roasted yerba mate (Ilex paraguariensis A. ST. Hil) consumption on glycemia and total serum creatine phosphokinase in patients with traumatic brain injury. J Funct Foods 28:240–245. https://doi.org/10.1016/j.jff.2016.11.024
Dartora N, De SLM, Santana-filho AP et al (2011) UPLC-PDA–MS evaluation of bioactive compounds from leaves of Ilex paraguariensis with different growth conditions, treatments and ageing. Food Chem 129:1453–1461. https://doi.org/10.1016/j.foodchem.2011.05.112
de Mejía EG, Song YS, Heck CI, Ramírez-Mares MV (2010) Yerba mate tea (Ilex paraguariensis): phenolics, antioxidant capacity and in vitro inhibition of colon cancer cell proliferation. J Funct Foods 2:23–34. https://doi.org/10.1016/j.jff.2009.12.003
de Resende PE, Kaiser S, Pittol V et al (2015) Influence of crude extract and bioactive fractions of Ilex paraguariensis A. St. Hil. (yerba mate) on the Wistar rat lipid metabolism. J Funct Foods 15:440–451. https://doi.org/10.1016/j.jff.2015.03.040
Derakhshan Z, Ferrante M, Tadi M et al (2018) Antioxidant activity and total phenolic content of ethanolic extract of pomegranate peels, juice and seeds. Food Chem Toxicol 114:108–111. https://doi.org/10.1016/j.fct.2018.02.023
Gullón B, Eibes G, Moreira MT et al (2018) Yerba mate waste: a sustainable resource of antioxidant compounds. Ind Crop Prod 113:398–405. https://doi.org/10.1016/j.indcrop.2018.01.064
Heck CI, Schmalko M, De Mejia EG (2008) Effect of growing and drying conditions on the phenolic composition of Mate teas (Ilex paraguariensis). J Agric Food Chem 56:8394–8403. https://doi.org/10.1021/jf801748s
Isolabella S, Cogoi L, López P et al (2010) Study of the bioactive compounds variation during yerba mate (Ilex paraguariensis) processing. Food Chem 122:695–699. https://doi.org/10.1016/j.foodchem.2010.03.039
Knapp MA, Santos DF dos, Pilatti-riccio D, et al (2019) Yerba mate extract in active starch films: Mechanical and antioxidant properties. J Food Process Preserv e13897:1–12. https://doi.org/10.1111/jfpp.13897
Kodama DH, de Gonçalves AESS, Lajolo FM, Genovese MI (2010) Flavonoids, total phenolics and antioxidant capacity: comparison between commercial green tea preparations. Ciência e Tecnol Aliment 30:1077–1082. https://doi.org/10.1093/oxfordjournals.pubmed.a024788
Meinhart AD, Caldeirão L, Damin FM et al (2018) Analysis of chlorogenic acids isomers and caffeic acid in 89 herbal infusions (tea). J Food Compos Anal 73:76–82. https://doi.org/10.1016/j.jfca.2018.08.001
Meinhart AD, Damin FM, Caldeirão L et al (2017) Chlorogenic acid isomer contents in 100 plants commercialized in Brazil. Food Res Int 99:522–530. https://doi.org/10.1016/j.foodres.2017.06.017
Nabechima GH, Provesi JG, Frescura JDO et al (2014) Thermal inactivation of peroxidase and polyphenoloxidase enzymes in mate leaves (Ilex paraguariensis) in a conveyor belt oven. CYTA J Food 12:399–406. https://doi.org/10.1080/19476337.2014.900115
Naczk M, Shahidi F (2006) Phenolics in cereals, fruits and vegetables: occurrence, extraction and analysis. J Pharm Biomed Anal 41:1523–1542. https://doi.org/10.1016/j.jpba.2006.04.002
Pagliosa CM, Vieira MA, Podestá R et al (2010) Methylxanthines, phenolic composition, and antioxidant activity of bark from residues from mate tree harvesting (Ilex paraguariensis A. St. Hil.). Food Chem 122:173–178. https://doi.org/10.1016/j.foodchem.2010.02.040
Pimenta AC, Silva PSDR, Zuffellato-Ribas KC, Koehler HS (2014) Caracterização de plantas e de frutos de araticunzeiro (Annona crassiflora mart.) nativos no cerrado matogrossense. Rev Bras Frutic 36:892–899. https://doi.org/10.1590/0100-2945-416/13
Signor P, Marcolini M (2017) Diagnóstico do consumo industrial de erva-mate no Paraná. Inst Florestas do Paraná
Zenebon O, Pascuet NS, Tiglea P (2008) Métodos físico-químicos para análise de alimentos, 4th edn. Instituto Adolfo Lutz, São Paulo
Funding
This research was funded by Universidade Federal da Fronteira Sul (UFFS) (PES-2019-0547) and by National Council for Scientific and Technological Development (CNPq) (432181/2018-0).
Author information
Authors and Affiliations
Contributions
All authors contribute to: Conceptualization, Methodology, Investigation, Writing—review & editing. V.Z. Pinto also was responsible to: Funding acquisition and Supervision.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare no conflict of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Pinto, V.Z., Pilatti-Riccio, D., Costa, E.S.d. et al. Phytochemical composition of extracts from yerba mate chimarrão. SN Appl. Sci. 3, 353 (2021). https://doi.org/10.1007/s42452-021-04373-2
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s42452-021-04373-2