Avoid common mistakes on your manuscript.
In 2016, Urtica incisa plants in a mixed crop field in the Salalah region of Oman showed typical symptoms of begomovirus infection. Twinned icosahedral particles (18–20 nm) were observed in symptomatic plant samples by electron microscopy. Total nucleic acid was extracted from leaves with the CTAB method (Porebski et al. 1997) and used in PCR with begomovirus specific primers TYLCD-356 (5′-ATCATTTCCACKCCCGYCTCGA-3′) and TYLCD-1044 (5′-GCRTGMGTACABGCCATATACA-3′). The expected 688 bp product was obtained from two symptomatic plants. The presence of a begomovirus DNA genome was confirmed by rolling-circle amplification (RCA) with the TempliPhi 100 Amplification System (GE Healthcare) and sequencing of ~2.7 kb HindIII-digested RCA products after cloning in pUC19 vector. The sequence of isolates Wed8–24 (MH475358) and Wed8–25 (MH475359) was 2759 bp in length. Pairwise sequence analysis by the Sequence Demarcation Tool (SDT) with the MUSCLE algorithm showed 99.6% nucleotide (nt) identity with each other (Muhire et al. 2014). The sequence of both isolates had the highest nt sequence identity (99.2%) with a chilli leaf curl virus (ChiLCV) isolate from tomato in Oman (JN604495). A betasatellite was also amplified from U. incisa samples by PCR using betasatellite specific primers Sat101 and Sat102 (Shahid et al. 2017). The amplified products of 1391 bp (MH475360) and 1386 bp (MH475361) were cloned in pTZ57R/T and sequenced. Sequences had a 99.7% nt identity and the highest nt sequence identity (98.6%) with a tomato leaf curl betasatellite (ToLCB) isolate from kidney bean in Oman (KX787940). No amplicons were recovered from U. incisa samples by PCR using primer pairs designed to amplify alphasatellites or DNA-B components. This is the first report of identification of ChiLCV and ToLCB in U. incisa from Oman.
References
Muhire BM, Varsani A, Martin DP (2014) SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PLoS One 9:e108277. https://doi.org/10.1371/journal.pone.0108277
Porebski S, Bailey LG, Baum BR (1997) Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Report 15:8–15
Shahid MS, Al-Sadi MA, Briddon RW (2017) First report of Chili leaf curl virus and associated betasatellite infecting watermelon (Citrullus lanatus) in Oman. Plant Dis 101:1063
Funding
This work was supported by the Sultan Qaboos University (Grant Code: SR/AGR/CROP/16/01 and IGR/AGR/CROP/17/02).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
Authors declare that they have no conflict of interest.
Ethical approval
This article does not contain any studies with human participants performed by any of the authors.
Rights and permissions
About this article
Cite this article
Shahid, M.S., Raza, A., Shafiq, M. et al. Infection of Urtica incisa with chili leaf curl virus and tomato leaf curl betasatellite in Oman. J Plant Pathol 101, 395 (2019). https://doi.org/10.1007/s42161-018-0168-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s42161-018-0168-x