Abstract
Talin protein was partially purified from human colorectal carcinoma tissues, which was subject to tryptic digestion. Immunoaffinity precipitation with specific antibodies that recognize diglycyl-lysine(Lys) remnants from tryptic digestion of ubiquitinated peptides was used to enrich ubiquitinated sites in talin. Mass spectrometry coupled with capillary reverse-phase high-performance liquid chromatography was used to analyze the enriched peptides. Specifically, four peptides containing diglycyl-Lys remnants from talin, namely, TAK(ub)VLVEDTK, QQQYK(ub) FLPSELRDEH, K(ub)STVLQQQYNR, and EGILK(ub)TAK can be determined using mass spectrometric data. This study provides an analytical method for further study in the relationship between ubiquitination modification of talin and its biological activity in colorectal cancer tissues with different pathological processes.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Zhang Y., Chen Z., Li J., Medicine(Baltimore), 2017, 96, e8242
Arnold M., Sierra M. S., Laversanne M., Soerjomataram I., Jemal A., Bray F., Gut, 2017, 66, 683
AI Bandar M. H., Kim N. K., Oncol. Rep., 2017, 37, 2553
Balacescu O., Sur D., Cainap C., Visan S., Cruceriu D., Man-zat-Saplacan R., Muresan M. S., Balacescu L., Lisencu C., Irimie A., Int. J. Mol. Sci., 2018, 19(12), 3711
Desiniotis A., Kyprianou N., Int. Rev. Cell. Mol. Biol., 2011, 289, 117
Yang H. J., Chen J. Z., Zhang W. L., Ding Y. Q., Cancer Invest., 2010, 28(2), 127
Chinthalapudi K., Rangarajan E. S., Izard T., Proc. Natl. Acad. Sci. USA, 2018, 115, 10339
Huang Z., Barker D., Gibbins J. M., Dash P. R., Exp. Cell Res., 2018, 370, 417
Yates M., Maréchal A., Int. J. Mol. Sci., 2018, 19(10), 2909
Abreha M. H., Dammer E. B., Ping L., Zhang T., Duong D. M., Gearing M., Lah J. J., Levey A. I., Seyfried N. T., Proteomics, 2018, 78, e1800108
Peng Q. S., Li G. P., Sun W. C., Yang J. B., Quan G. H., Liu N., Chinese Journal of Analytical Chemistry, 2016, 44, 850
Huang C., Rajfur Z., Yousefi N., Chen Z., Jacobson K., Ginsberg M. H., Nat. Cell Biol., 2009, 11 624
Liu N., Song W., Wang P., Lee K. C., Cai Z., Chen H., Proteomics, 2010, 10, 1875
Liu N., Song W., Lee KC., Wang P., Chen H., Cai Z., J. Am. Soc. Mass Spectrom., 2009, 20, 312
Cheng W., Zhan F. L., Li S. M., Yang J. B., Wang Y., Liu N., Chinese Journal of Analytical Chemistry, 2019, 47, 30
Murphy S., Zweyer M., Henry M., Meleady P., Mundegar R. R., Swandulla D., Ohlendieck K., Clin. Proteomics, 2018, 75, 34
Yang G. Y., Zhao H. F., Xie F., Li Y., Sun L., Shi Q. H., He C. Y., Liu N., Song L. N., Chem. Res. Chinese Universities, 2012, 28(6), 1031
Yang H., Su R., Wishnok J. S., Liu N., Chen C., Liu S., Tannenbaum S. R., Mikrochim. Acta, 2019, 786, 104
Liu N., Zhao L. C., He C. Y., Cai Z. W., Curr. Anal. Chem., 2012, 8, 22
Feng S., Pan C., Jiang X., Xu S., Zhou H., Ye M., Zou H., Proteomics, 2007, 7, 351
Pascovici D., Wu J. X., McKay M. J., Joseph C., Noor Z., Kamath K., Wu Y., Ranganathan S., Gupta V., Mirzaei M., Int. J. Mol. Sci., 2019, 20(1), 16
Author information
Authors and Affiliations
Corresponding authors
Additional information
Supported by the Fund of Science and Technology Department of Jilin Province, China(No.20150414015GH) and the National Natural Science Foundation of China(Nos.81572082, 81472454).
Rights and permissions
About this article
Cite this article
Wang, K., Qiao, L., Li, X. et al. Profiling of Ubiquitination Modification Sites in Talin in Colorectal Carcinoma by Mass Spectrometry. Chem. Res. Chin. Univ. 35, 377–381 (2019). https://doi.org/10.1007/s40242-019-8377-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s40242-019-8377-1