Abstract
Magnetic Fe3O4@SiO2 nanoparticles with superparamagnetic properties were prepared via a reverse microemulsion method at room temperature. The as-prepared samples were characterized by transmission electron microscopy(TEM), X-ray diffractometry(XRD), and vibrating sample magnetometry(VSM). The Fe3O4@SiO2 nanopar-ticles were modified by (3-aminopropyl)triethoxysilane(APTES) and subsequently activated by glutaraldehyde(Glu). Protein A was successfully immobilized covalently onto the Glu activated Fe3O4@SiO2 nanoparticles. The adsorption capacity of the nanoparticles was determined on an ultraviolet spectrophotometer(UV) and approximately up to 203 mg/g of protein A could be uniformly immobilized onto the modified Fe3O4@SiO2 magnetic beads. The core-shell of the Fe3O4@SiO2 magnetic beads decorated with protein A showed a good binding capacity for the chimeric anti-EGFR monoclonal antibody(anti-EGFR mAb). The purity of the anti-EGFR mAb was analyzed by virtue of HPLC. The protein A immobilized affinity beads provided a purity of about 95.4%.
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Supported by the National Natural Science Foundation of China(Nos.51572058, 91216123, 51174063), the Natural Science Foundation of Heilongjiang Province, China(No.E201436), the International Science & Technology Cooperation Program of China(Nos.2013DFR10630, 2015DFE52770) and the Specialized Research Fund for the Doctoral Program of Higher Education of China(No.SRFDP 20132302110031).
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Hou, X., Zhao, C., Tian, Y. et al. Preparation of functionalized Fe3O4@SiO2 magnetic nanoparticles for monoclonal antibody purification. Chem. Res. Chin. Univ. 32, 889–894 (2016). https://doi.org/10.1007/s40242-016-6251-y
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DOI: https://doi.org/10.1007/s40242-016-6251-y