Abstract
Anthocyanins are responsible for most of the bright red and blue colors found in higher plants. Anthocyanin accumulation is regulated by MYB transcription factors. Some varieties of apple indicate anthocyanin accumulation with a red color in the skin and, at times, even in the cortex core, but other varieties without anthocyanin production have green-colored skin indicative of chlorophyll accumulation. To obtain gene expression profiles from the apple cultivars with different flesh and skin colors, 2 apple cultivars, the red-colored ‘Redfield’ and the green-colored ‘Greensleeves’, were used. A cDNA suppression subtractive hybridization library was established and analyzed. Six genes encoding methallothionein-like protein, chalcone isomerase, dirigent-like protein, brassinosteroid-6-oxidase, an unnamed protein product, and some unknown proteins were selected, and their expression was confirmed in the ‘Redfield’ cultivar through virtual northern blot analysis. In the ‘Greensleeves’ cultivar, 5 genes encoding gibberellins-regulated protein, a hypothetical 23.5 KD protein, anthranilate N-hydroxycinnamoyl/benzoyltransferase, and isoflavone reductase were chosen, and their upregulated transcriptional levels were identified. These selected genes were differently expressed in each apple cultivar, suggesting that these genes directly or indirectly regulate anthocyanin accumulation.
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Literature Cited
Adams-Phillips, L., C. Barry, and J. Giovannoni. 2004. Signal transduction systems regulating fruit ripening. Trends Plant Sci. 9:331–338.
Alba, R., P. Payton, Z. Fei, R. McQuinn, P. Debbie, G.B. Martin, S.D. Tanksley, and J.J. Giovannoni. 2005. Transcriptome and selected metabolites analyses reveal multiple points of ethylene control during tomato fruit development. Plant Cell 17:2954–2965.
Ban, Y., C. Honda, Y. Hatsuyama, M. Igarashi, and H. Bessho. 2007. Isolation and functional analysis of a MYB transcription factor gene that is a key regulator for the development of red coloration in apple skin. Plant Cell Physiol. 48:958–970.
Bednar, R.A. and G.C. Hadcock. 1988. Purification and characterization of chalcone isomerase from soybeans. J. Biol. Chem. 263: 9582–9588.
Chervin, C., A. El-Kereamy, J.P. Roustan, L. Latche, J. Lamon, and M. Bouzayen. 2004. Ethylene seems required for the berry development and ripening in grape, a non-climacteric fruit. Plant Sci. 167:1301–1305.
Davies, C. and S.P. Robinson. 2000. Differential screening indicates a dramatic change in mRNA profiles during grape berry ripening. cloning and characterization of cDNA encoding putative cell wall and stress response proteins. Plant Physiol. 122:803–812.
Eberhardt, M.V., C.Y. Lee, and R.H. Liu. 2000. Antioxidant activity of fresh apples. Nature 405:903–904.
Espley, R., R.P. Hellens, J. Putterill, D.E. Stevenson, S. Kutty-Amma, and A.C. Allan. 2007. Red colouration in apple fruit is due to the activity of the MYB transcription factor. MdMYB10. Plant J. 49:414–427.
Giovannoni, J. 2001. Molecular biology of fruit maturation and ripening. Annu. Rev. Plant Physiol. Plant Mol. Biol. 52:725–749.
Giovannoni, J. 2004. Genetic regulation of fruit development and ripening. Plant Cell 16:S170–S180.
Goulao, L.F. and C.M. Oliveira. 2007. Molecular identification of novel differentially expressed mRNAs up-regulated during ripening of apples. Plant Sci. 172:306–318.
Habu, Y., Y. Hisatomi, and S. Lida. 1998. Molecular characterization of the mutable flaked allele for flower variegation in the common morning glory. Plant J. 16:371–376.
Kim, M.K., J.H. Jeon, L.B. Davin, and N.G. Lewis. 2002. Monolignol radical-radical coupling networks in western red cedar and Arabidopsis and their evolutionary implications. Phytochemistry 61:311–322.
Kim, S., R. Jones, K.S. Yoo, and L.M. Pike. 2004. Gold color in onions (Allium cepa): A natural mutation of the chalcone isomerase gene resulting in a premature stop codon. Mol. Gen. Genomics 272:411–419.
Knekt, P., J. Kumpulainen, J.H. Rissanen, M. Heliovaara, A. Reunanen, T. Hakulinen, and A. Aromaa. 2002. Flavonoid intake and risk of chronic diseases. Amer. J. Cli. Nutr. 76:560–568.
Kobayashi, S., M. Ishimaru, C.K. Ding, H. Yakushiji, and N. Goto. 2001. Comparison of UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT) gene sequences between white grapes (Vitis vinifera) and their sports with red skin. Plant Sci. 160:543–550.
Kortekamp, A. 2006. Expression analysis of defence-related genes in grapevine leaves after inoculation with a host and a non-host pathogen. Plant Physiol. Biochem. 44:58–67.
Lelievre, J.M., A. Latche, B. Jones, M. Bouzayen, and J.C. Pech. 1997. Ethylene and fruit ripening. Physiol. Plant 101:727–739.
Liu, P., C.J. Goh, C.S. Loh, and E.C. Pua. 2002. Differential expression and characterization of three methallothionein-like genes in Cavendish banana (Musa acuminate). Physiol. Plant 114:241–250.
Loreti, E., G. Povero, G. Novi, C. Solfanelli, A. Alpi, and P. Perata. 2008. Gibberellins, jasmonate and abscisic acid modulate the sucrose-induced expression of anthocyanin biosynthetic genes in Arabidopsis. New Phytologist 179:1004–1016.
Nam, Y.W., L. Tichit, M. Leperlier, B. Cuerq, I. Marty, and J.M. Leliever. 1999. Isolation and charaterization of mRNAs differentially expressed during ripening of wild strawberry (Fragaria vesca L.) fruits. Plant Mol. Biol. 39:629–636.
Newcomb, R.D., R.N. Crowhurst, A.P. Gleave, E.H.A. Rikkerink, A.C. Allan, L.L. Beuning, J.H. Bowen, E. Gera, K.R. Jamieson, B.J. Janssen, W.A. Laing, S. McArtney, B. Nain, G.S. Ross, K.C. Snowden, E.J.F. Souleyre, E.F. Walton, and Y.K. Yauk. 2006. Analysis of expressed sequence tags from apple. Plant Physiol. 141:147–166.
Nisahiro, M., T. Nakatsuka, and S. Yamamura. 2005. Flavonoid component and flower color change in transgenic tobacco plants by suppression of chalcone isomerase gene. FEBS Lett. 579:6074–6078.
Park, S., N. Sugimoto, M.D. Larson, R. Beaudry, and S. van Nocke. 2006. Identification of genes with potential roles in apple fruit development and biochemistry through large-scale statistical analysis of expressed sequence tags. Plant Physiol. 141:811–824.
Reid, S.J. and G.S. Ross. 1997. Up-regulation of two cDNA clones encoding methallothionein-like proteins in apple fruit during cool storage. Physiol. Plant 100:183–189.
Seymour, G.B., J.E. Taylor, and G.A. Tucker. 1993. Biochemistry of fruit ripening. Chapman & Hall, London.
Shih, C.H., I.K. Chu, W.K. Yip, and C. Lo. 2006. Differential expression of two flavonoid 3’-hydroxylase cDNAs involved in biosynthesis of anthocyanins pigments and 3-deoxyanthocyanidin phytoalexins in sorghum. Plant Cell Physiol. 47:1412–1419.
Solfanelli, C., A. Poggi, E. Loreti, A. Alpi, and P. Perata. 2006. Sucrose-specific induction of the anthocyanins biosynthesis pathway in Arabidopsis. Plant Physiol. 140:637–646.
Symons, G.M., C. Davies, Y. Shavrukov, I.B. Dry, and J.B. Reid. 2006. Grapes on steroids. Brassinosteroids are involved in grape berry ripening. Plant Physiol. 140:150–158.
Takos, A., F.W. Jaffe, S.R. Jacob, J. Bogs, S.P. Robison, and A.R. Walker. 2006. Light-induced expression of a MYB gene regulates anthocyanins biosynthesis in red apples. Plant Physiol. 142:1216–1232.
Tanksley, S.D. 2004. The genetic, developmental and molecular bases of fruit size and shape variation in tomato. Plant Cell 16:S181–S189.
Theologis, A. 1992. One rotten apple spoils the whole bushel: the role of ethylene in fruit ripening. Cell 70:81–184.
Toufektsian, M.C., M.D. Lorgeril, N. Nagy, P. Salen, M.B. Donati, L. Giordano, H.P. Mock, S. Peterek, A. Matro, K. Petroni, R. Pilu, D. Rotilio, C. Tonelli, J.D. Leiris, F. Boucher, and C. Martin. 2008. Chronic dietary intake of Plant-derived anthocyanins protects the rat heart against ischemia-reperfusion injury. J. Nutr. 138:747–752.
Ubi, B.E., C. Honda, H. Bessho, S. Kondo, M. Wada, S. Kobayashi, and T. Moriguchi. 2006. Expression analysis of anthocyanin biosynthetic genes in apple skin: Effect of UV-B and temperature. Plant Sci. 170:571–578.
Wu, Q. and H.D. VanEtten. 2004. Introduction of Plant and Fungal Genes intp Pea (Pisum sativum L.) Hairy Roots Reduces Their Ability to Produce Pisatin and Affects Their Response to a Fungal Pathogen. MPMI 17:798–804.
Yamazaki, M., M. Shibata, Y. Nishiyama, K. Springob, M. Kitayama, N. Shimada, T. Aoki, S.I. Ayabe, and K. Saito. 2008. Differential gene expression profiles of red and green forms of Perilla frutescens leading to comprehensive identification of anthocyanins biosynthetic genes. FEBS J. 275:3494–3502.
Yang, Q., B. Grimming, and U. Matern. 1998. Anthranilate N-hydroxycinnamoyl/benzoyltansferase gene from carnation: rapid elicitation of transcription and promoter analysis. Plant Mol. Biol. 38:1201–1214.
Yi, H.C., S. Joo, K.H. Nam, B.G. Kang, and W.T. Kim. 1999. Auxin and brassinosteroid differentially regulated the expression of three members of the 1-aminocyclopropane-1-carboxylate synthesis gene family in mung bean (Vigna radiate L.). Plant Mol. Biol. 41:443–454.
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Han, S.E., Lee, H.E., Heo, S. et al. Isolation and characterization of genes expressed differently in mature fruits of ‘redfield’ and ‘greensleeves’ apples. Hortic. Environ. Biotechnol. 52, 413–421 (2011). https://doi.org/10.1007/s13580-011-0213-6
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DOI: https://doi.org/10.1007/s13580-011-0213-6