Abstract
The molecular genetic diversity of 404 indigenous landraces from sesame core collection in China were evaluated by 11 SRAP and 3 SSR markers, 175 fragments were generated, of which 126 were polymorphic with an average polymorphism rate of 72%. Jaccard’s genetic similarity coefficients (GS=0.7130), Nei’s gene diversity (h=0.2418) and Shannon’s Information index (I=0.3847) were calculated, a dendrogram of the 404 landraces was made, landraces from various zones were distributed throughout the dendrogram, accessions from different agro-ecological zones were indistinguishable by cluster analysis, geographical separation did not generally result in greater genetic distance, a similar pattern was obtained using principal coordinates (PCO) analysis. As to seven agro-ecological zones, the maximum Nei’s gene diversity (h = 0.2613) and Shannon index (I = 0.3980) values in zone VII indicated that they were genetically more diverse than those in other zones, while the least genetically diverse region was zone III (h = 0.1772, I = 0.2858). Nei’s genetic identity and genetic distance among landraces from seven agro-ecological zones were also analyzed, the genetic relationship of seven zones was inferred using the UPGMA method. This study demonstrated that SRAP and SSR markers were appropriate for evaluation of sesame genetic diversities. There existed extensive genetic diverse among indigenous landraces and the abundance of genetic diversity of landraces in different agro-ecological zones was various. Understanding of these characteristics of indigenous landraces in China can provide theoretical foundation for further collection, effective protection and reasonable utilization of these sesame landraces in breeding.
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References
Bhat V, Babrekar P and Lakhanpaul S (1999) Study of genetic diversity in Indian and exotic sesame (Sesamum indicum L.)germplasm using random amplified polymorphic DNA (RAPD) markers. Euphytica 110: 21–33.
Chen CY (1981) The Catalogue of Sesame Germplasm Research in China. Agricultural Scientech Press, Beijing.
Dixit A, Jin MH, Chung JW, Yu JW, Chung HK, Ma KH, Park YJ and Cho EG (2005) Development of polymorphic microsatellite markers in sesame (Sesamum indicum L.). Mol. Ecol. Notes 5: 736–738.
Dong ZX (1990) Identification of Chinese sesame germplasm resistance to Fusarium oxysporium. Oil Crops China 3: 74–76.
Doyle JJ and Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11–15.
Ercan AG, Taskin M and Turgut K (2004) Analysis of genetic diversity in Turkish sesame (Sesamum indicum L.) populations using RAPD markers. Genet. Resour. Crop Evol. 51: 599–607.
Feng XY (1992) The Catalogue of Sesame Germplasm Research in China (continuation 1). Agricultural Scientech Press, Beijing.
Feng XY (1997) The Catalogue of Sesame Germplasm Research in China (continuation 1). Agricultural Scientech Press, Beijing.
Feng XY, Zhang XR and Liu YY (1996) Further characterization and evaluation of pre-selected sesame germplasm. Oil Crops China 18: 63–66.
Feng XY, Zhang XR and Xiao TH (1991) Identification and evaluation of Chinese sesame germplasm tolerance to water logging stress. Oil Crops China 3: 12–15.
Ferdinandez YSN, Somers DJ and Coulman BE (2001) Estimation the genetic relationship of hybrid bromegrass to smooth bromegrass and meadow bromegrass using RAPD markers. Plant Breed. 120: 149–153.
Hawkes J (1983) The diversity of crop plants. Harvard University Press, Cambridge.
Hernan EL and Petr K (2006) Genetic relationship and diversity in a sesame (Sesamum indicum L.) germplasm collection using amplified fragment length polymorphism (AFLP). BMC Genet. 7: 1–10.
Isshiki S and Umezake T (1997) Genetic variations of isozymes in cultivated sesame. Euphytica 93: 375–377.
Kim DH, Zur G, Danin Y, Lee SW, Shim KB, Kang CW and Kashi Y (2002) Genetic relationships of sesame germplasm collection as revealed by inter-simple sequence repeats. Plant Breed. 121: 259–262.
Li G, Quiros CF (2001) Sequence-related amplified polymorphism (SRAP) a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor. Appl. Genet. 103: 455–461.
Li LL, Wang SY and Fang XP (1991) Identification of Chinese sesame germplasm resistance to Macrophomina phaseolina. Oil Crops China 1: 3–6.
Lin ZX, He DH, Zhang XL, Nie YC, Guo XP, Feng CD and Stewart JM (2005) Linkage map construction and mapping QTL for cotton fiber quality using SRAP, SSR and RAPD. Plant Breed. 124: 180–187.
Pham TD, Bui TM, Werlemark G, Bui TC, Merker A and Carlsson AS (2009) A study of genetic diversity of sesame (Sesamum indicum L.) in Vietnam and Cambodia estimated by RAPD markers. Genet. Resour. Crop Evol. 56: 679–690.
Powell W, Machray GC and Provan J (1996) Polymorphism revealed by simple sequence repeats. Trends Plant Sci. 1: 215–222.
Qamaruz ZF, Michael FF and Parker JS (1998) Molecular techniques employed in the assessment of genetic diversity: A review focusing on orchid conservation. Lindleyana 13: 259–283.
Rohlf FJ (2000) NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, Version 2.1, User Guide. Exeter Software, New York.
Sneath PH and Sokal RR (1973) Numerical Taxonomy: The Principal and Practice of Numerical Classification. W. H. Freeman and Company, San Francisco.
Sokal RR, Michener CD (1958) A statistical method for evaluating systematic relationships. Univ. Kansas Sci. Bull. 28: 1409–1438.
Tamura K, Dudley J and Nei M (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Bio. Evo. 24: 1596–1599.
Venkataramana BK, Prashant BP and Suman L (1999) Study of genetic diversity in Indian and exotic sesame (Sesamum indicum L.) germplasm using random amplified polymorphic DNA (RAPD) markers. Euphytica 110: 21–33.
Xiao TH, Feng XY and Zhang XR (1992) Analysis of the distribution and main characteristics of black seed coat sesame germplasm in China. Oil Crops China 14: 34–37.
Yeh FC and Boyle TJB (1997) Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belgium J. of Botany 129: 157.
Zeven A and Zhukovsky P (1975) Dictionary of cultivated plants and their centres of diversity. PUDOC, Wageningen.
Zhang XR, Cheng Y, Liu SY, Feng XY, Jin LM, Jin QL, Huang CY, Wang ZA, Xu XF and Sun MY (2001) Evaluation of sesame germplasm resistance to Macrophomina phaseolina and Fusarium oxysporium. Chin. J. Oil Crop Sci. 23: 23–27.
Zhang XR, Zhao YZ, Cheng Y Feng XY, Guo QY, Zhou MD and Hodgkin T (2000) Establishment of sesame germplasm core collection in China. Genet. Resour. Crop Evol. 47: 273–279.
Zhang YX, Lin ZX, Xia QZ, Zhang MJ and Zhang XL (2008) Characteristics and analysis of simple sequence repeats in the cotton genome based on a linkage map constructed from a BC1 population between Gossypium hirsutum and G. barbadense. Genome 51: 534–546.
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Zhang, Yx., Zhang, Xr., Hua, W. et al. Analysis of genetic diversity among indigenous landraces from sesame (Sesamum indicum L.) core collection in China as revealed by SRAP and SSR markers. Genes Genom 32, 207–215 (2010). https://doi.org/10.1007/s13258-009-0888-6
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DOI: https://doi.org/10.1007/s13258-009-0888-6