Abstract
The replacement of native species by invasive Phragmites australis in coastal wetlands may impact ecosystem processes including fluxes of the greenhouse gases (GHGs) carbon dioxide (CO2) and methane (CH4). To investigate differences in daytime CH4 and CO2 fluxes as well as vegetation properties between Phragmites and native vegetation zones along a salinity gradient, fluxes were measured via cavity ringdown spectroscopy in 3 New England coastal marshes, ranging from oligohaline to polyhaline. While daytime CH4 emissions decreased predictably with increasing soil salinity, those from Phragmites zones were larger (15 to 1254 μmol m−2 h−1) than those from native vegetation (4–484 μmol m−2 h−1) across the salinity gradient. Phragmites zones displayed greater daytime CO2 uptake than native vegetation zones (−7 to −15 μmol m−2 s−1 vs. -2 to 0.9 μmol m−2 s−1) at mesohaline-polyhaline, but not oligohaline, sites. Results suggest that vegetation zone and salinity both impact net emission or uptake of daytime CO2 and CH4 (respectively). Future research is warranted to demonstrate Phragmites-mediated impacts on GHG fluxes, and additional measurements across seasonal and diel cycles will enable a more complete understanding of Phragmites' net impact on marsh radiative forcing.
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Introduction
Invasive plants can alter the structure and function of coastal wetlands. Exotic species invasions of coastal wetlands are known drivers of ecosystem-level change including impacts to vegetation (Bertness et al. 2002) and microbial (Ravit et al. 2003) community structure, trophic function (Levin et al. 2006), and biogeochemistry (Windham and Ehrenfeld 2003; Tong et al. 2012). Invasive plants can alter components of nitrogen (N), carbon (C), and water cycling via differences in productivity and rhizosphere conditions including nutrient uptake, soil oxygenation, and availability of C exudates relative to native plants (Ehrenfeld 2003).
In North American coastal wetlands, the presence of the invasive grass Phragmites australis has increased steadily in recent decades (Chambers et al. 1999; Meyerson et al. 2009) with potential implications for ecosystem function. Phragmites has been shown to outcompete native species by shading (Chambers et al. 1999), capitalizing on nutrient availability (Mozdzer and Zieman 2010), and rapidly spreading by both clonal replication (Amsberry et al. 2000) and seeding (McCormick et al. 2009). Although Phragmites invasion is known to exclude native high marsh vegetation (Minchinton et al. 2006) and therefore reduce species richness in the high marsh community (Silliman and Bertness 2004), ways in which ecosystem functions may be affected by Phragmites invasion are less well-understood.
The replacement of native vegetation with invasive Phragmites-dominated communities (hereafter Phragmites zones) can mediate significant shifts in net CO2 and CH4 fluxes. Generally, coastal wetlands emit minimal carbon dioxide (CO2) and methane (CH4) (Mitsch and Gosselink 2000; Poffenbarger et al. 2011; Madigan 2012). Phragmites may increase marsh CO2 uptake in the short term due to its greater productivity relative to smaller native species (Windham 1999). It may also contribute to decreased CH4 emissions as a result of rhizosphere methanotrophy, since Phragmites' physiology often leads to a notable oxygenation of its rhizosphere (Armstrong 2000; Colmer 2003). However, invasive Phragmites also has the potential to exacerbate marsh CH4 emissions relative to native vegetation (Mozdzer and Megonigal 2013). Phragmites' provision of labile organic C to its rhizosphere (Ravit et al. 2003; Lovell 2005; Armstrong et al. 2006) may result in increased methanogen presence or activity. Phragmites may also directly drive CH4 emissions since its pressure-driven transport system is known to conduct rhizosphere-derived CH4 (Brix et al. 2001) through the plants' massive aerenchyma and into the atmosphere (Armstrong et al. 1996; Grosse et al. 1996; Beckett et al. 2001; Colmer 2003). Characterization of CO2 and CH4 fluxes from marshes vegetated with invasive Phragmites and native vegetation could provide insight into potential impacts of Phragmites invasion on marsh GHG flux dynamics. However, few have performed such investigations (Emery and Fulweiler 2014), and no studies to date compare greenhouse gas (GHG) fluxes from Phragmites and the high marsh native perennials (such as Spartina patens, Distichlis spicata and Juncus gerardii) that Phragmites is likely to displace as it invades from upland borders and along creek banks (Chambers et al. 1999; Silliman and Bertness 2004).
Factors other than vegetation type also affect marsh GHG fluxes, and must be taken into account when attempting to discern impacts of changing plant communities. Salinity is known to be a major control on CH4 fluxes in coastal wetlands (Poffenbarger et al. 2011) as frequent inundation with seawater replenishes sulfate to soil bacterial communities that outcompete methanogenic archaea (Mitsch and Gosselink 2000; Madigan 2012). Salinity also is understood to constrain Phragmites distribution (Burdick et al. 2001), and while Phragmites is capable of growing at marine strength salinities (Chambers et al. 2003), it often displays reduced vigor and success when exposed to increased seawater inundation (Hanganu et al. 1999). A comparison of GHG fluxes from Phragmites and native vegetation stands along a natural salinity gradient could begin to distinguish between biotic and abiotic controls on flux differences.
The objective of this research was to compare CH4 and CO2 fluxes from Phragmites and native high marsh vegetation zones during a growing season in three Southern New England coastal marshes that span a natural salinity gradient. Surface soil properties, plant variables and pore water sulfide also were measured and tested for their relationship to observed GHG fluxes. Phragmites zones were hypothesized to have higher CH4 emissions but also higher rates of CO2 uptake than native high marsh zones given the plant’s greater gas transport and productivity rates. CH4 fluxes were expected to decrease with increasing marsh salinity in both Phragmites and native high marsh zones.
Materials and Methods
Study Sites
Study sites were chosen to allow for comparison of GHG fluxes between Phragmites and native high marsh vegetation zones along a salinity gradient. Three Phragmites-invaded southern New England coastal wetlands were selected: two in lower Narragansett Bay, Rhode Island (Round Marsh and Fox Hill) and one in Waquoit Bay, Massachusetts (Sage Lot) (Table 1, Fig. 1). Sage Lot is located in a watershed with minimal development and minimal N loads (Valiela and Cole 2002), and Round Marsh and Fox Hill are located in lower Narragansett Bay where N concentrations from anthropogenic activities are estimated to be low (Wigand et al. 2003). Native vegetation consists primarily of Spartina patens and Distichlis spicata (see Table 1 for details), and Phragmites invasion encroaches from the upland edge of all marsh sites. Sites increase in growing season surface soil salinity from oligohaline levels in Round Marsh to polyhaline in Sage Lot, with Fox Hill being intermediate (Table 1). All sites experienced flooding with 32 ppt seawater in both vegetation zones during spring tides.
Experimental Design
At each site, GHG fluxes were compared between the Phragmites and native high marsh vegetation zones using three replicate plots per zone. At Round Marsh, three plots were selected in each vegetation zone with approximately 30 m of spacing between plots. At Fox Hill and Sage Lot, three plots were selected in the native high marsh zone, but in the Phragmites zone where pilot trials indicated high gas flux variability, three pairs of plots (with 0.3 m spacing between paired plots) were established (data from the paired plots were averaged prior to statistical analyses, as detailed below). In Phragmites zones, plots were established approximately 1 m from the leading, seaward edge of the stand, and in native vegetation zones plots were placed at random. Phragmites-mediated changes to edaphic conditions and microbial communities at the leading edge of the invasion have persisted for shorter duration than in older, interior portions of the stand. Therefore, comparisons between native and Phragmites zones represent conservative estimates of potential changes in GHG flux dynamics due to invasion.
For GHG flux measurements, bases were installed in each selected plot to support static flux chambers. Bases were installed in the early spring (at least 2 weeks prior to first measurements) so as to permit recovery of vegetation, and were left in place for the duration of the growing season to minimize soil disturbance. Phragmites zone chamber bases consisted of PVC rings (24 cm tall × 30 cm diameter), and native vegetation chamber bases consisted of stainless steel rings (9 cm tall × 30 cm diameter). Phragmites and native vegetation bases were inserted 8 cm into the soil and both types featured drainage holes positioned just beneath the soil surface to allow for lateral water movement after tidal inundation or rainfall.
Edaphic Variable and Plant Metrics
Surface soil (top 3 cm) salinities were measured at each plot within chamber bases once per month from April-August 2014 at the time of GHG flux measurements. Soil was pressed against paper filters using small syringes to extract water, which was analyzed for salinity using a handheld refractometer. Soil pore water was collected at each site in May, July and late August from each plot during GHG flux measurements using 15 cm Rhizon Soil Moisture Samplers (Ben Meadows, Janesville, WI), preserved using 1 M zinc acetate, and frozen until analysis. Pore water sulfide concentrations were analyzed using standard colorimetric techniques (Cline 1969). Mid-growing season soil pH, oxidation-reduction potential, temperature and moisture point measurements were performed just outside each chamber base once each during June and July GHG flux measurements for a total of two measurements per plot. Soil oxidation-reduction (redox) potential (ORP) and pH were measured using an ORP probe and pH/ORP meter (Mettler Toledo, Greifensee, Switzerland) and pH meter (ExStick® Instruments, Nashua, NH). Soil temperature was measured with a digital thermometer inserted into soil at a depth of 15 cm, and soil moisture content was measured using a volumetric water content sensor (Decagon Devices, Pullman, WA) inserted 5 cm into soil. Soil for organic content analysis was collected from each plot in August. Using a cutoff syringe, approximately 10 mL of soil was collected. Soil was dried, weighed, and placed in a muffle furnace at 500 °C overnight. Change in weight was defined as loss on ignition (LOI), a proxy for soil organic content.
To characterize Phragmites vegetation at each site, densities of live and dead stems and average stem height within chamber bases were recorded during the growing season once plants were mature (July). For stem height, ten stems per plot were selected randomly and their heights were averaged.
GHG Flux Measurements
Daytime GHG flux measurements were performed during the early (April-May), mid- (June-July), and late (late August) growing season during 2014. Gas measurements were conducted for 6–10 min per plot, based on observed periods for linear rates of change. All GHG flux measurements were performed between 9:00 AM and 3:00 PM and within 3 h of low tide.
A cavity ring down spectroscopy (CRDS) analyzer (Picarro G2508) was used to measure CO2, CH4 and N2O concentrations in real-time. The analyzer cavity, together with a flux chamber and connective tubing, creates a closed system within which gas concentration changes over time are measured with a flow rate of ~230 sccm and frequency of approximately 1 measurement/s. The gas analyzer simultaneously measures H2O vapor concentrations and reports the dry mole fraction of the other target gases, and this corrected value was used for all flux calculations. H2O vapor saturations never exceeded 2.5 % over the course of any measurements.
The analyzer was connected via nylon tubing to transparent polycarbonate chambers, which were placed into the previously-installed bases. Vegetation was left intact inside the chambers. A 0.02 m3 polycarbonate chamber was used for native species zone measurements following previously-described methods (Moseman-Valtierra et al. 2011). In order to accommodate tall (up to 2 m) Phragmites plants, a 2 m tall, 0.3 m diameter transparent polycarbonate tube (Rideout PlasticsR) was modified to extend the shorter polycarbonate chamber which was sealed to the extender using a polyethylene closed-cell foam collar with its channel filled with water (for a total chamber volume of 0.15 m3). Extender support bases were designed to create a gas-tight fit between base and extender. Two small fans attached to the inside of the polycarbonate chamber (10-cm fans) and extender (20-cm fans) ensured air mixing during measurements. A stainless steel 55 cm long, 0.8 mm diameter pigtail was used for pressure equilibration. Hobo® data loggers (Onset, Bourne, MA) were suspended within chambers during all flux measurements to monitor air temperature at 30 s intervals.
GHG fluxes were calculated using chamber size and footprint. The Ideal Gas Law (PV = nRT) was used to calculate changes in gas concentrations over time using field-measured air temperatures and atmospheric pressure. Cases in which no change in gas concentration over time was detectable for the duration of the measurement period were classified as having a flux of 0 (3.8 % of CO2 and 7.6 % of CH4 measurements). When slopes had an R2 value of less than 0.85, data were not included in the analysis (5.7 % of CO2 and 1.9 % of CH4 measurements). The relatively short time period of these greenhouse gas flux measurements are not designed to capture ebullitive fluxes, and thus may represent underestimates of total gas emissions, particularly for CH4 (Tokida et al. 2005). However, our high resolution measurement of gas concentrations does enable detection of the rapid (often step-shaped) changes in gas concentrations that occur during ebullition to be very well resolved and distinguished from diffusive flux during the periods of chamber deployment (Middelburg et al. 1996). We did not detect ebullition from this dataset.
Statistical Analyses
When two sets of measurements were taken during a portion of the growing season (n = 3 seasonal stages: early, mid or late), averages for the two sampling dates were computed by plot. At Fox Hill and Sage Lot where Phragmites zone measurements were conducted in duplicate, averages of measurements from pairs of plots were used for statistical analyses. Therefore, for GHG flux, soil salinity and porewater sulfide data, each site had three data points per vegetation zone per growing season period for a total of 18 data points per site. CO2 and CH4 fluxes, soil salinity and porewater sulfide were compared between vegetation zones at each site using a two-factor ANOVA with vegetation type and growing season period (early, mid, late) as the two factors, and comparisons were drawn between sites using a one-factor ANOVA.
For edaphic variables, June and July data were averaged for the two sampling dates by plot. Edaphic and plant variable data collected from pairs of Phragmites plots were averaged and the means of the two values were used for statistical analysis. Therefore, for pH, redox potential, temperature, moisture, and soil organic C, each site had n = 3 data points per vegetation zone for the mid growing season only (for a total of six data points per site). Edaphic variables were compared using two-factor ANOVA with site and vegetation type as main effects. Phragmites vegetation characteristics (stem height, live and dead stem counts) were compared between sites using a one-factor ANOVA. Data were aligned then rank-transformed prior to ANOVA analyses (Salter and Fawcett 1993; Wobbrock et al. 2011) to account for deviations in normality while allowing for tests of effect interaction (Seaman et al. 1994). Tukey’s HSD test was used for post-hoc pairwise comparisons when appropriate.
Potential relationships between edaphic and vegetation variables and GHG fluxes were investigated using Spearman’s R Correlation Test.
All statistics were performed in R (R Core Team 2014) and interpreted at a significance level of 0.05.
Results
Edaphic Variables & Vegetation Characteristics
Confirming the expected salinity gradient, soil salinity differed significantly between all 3 sites and was highest at Sage Lot and lowest at Round Marsh, with Fox Hill intermediate (Table 2). Significant differences in salinity between vegetation zones (Phragmites and native) were present only at Fox Hill (Table 2), with salinity higher by several ppt in the native vegetation zone during the mid and late growing season stages.
Porewater sulfide concentrations ranged from 0 to approximately 250 μM, although one sample (from native vegetation at Sage Lot) had a sulfide concentration of over 1000 μM (Table 2). Concentrations did not differ significantly between vegetation zones at any site, but did display between-site differences when averaged across all dates, with Sage Lot sulfide concentrations (139.00 ± 17.77) significantly greater than those at Round Marsh (31.57 ± 16.31) (Table 2).
Surface soil pH averaged across vegetation zones at Sage Lot was significantly greater than at Round Marsh and Fox Hill (Table 3). Surface soil oxidation-reduction potential averaged across vegetation zones was significantly lower at Sage Lot than Round Marsh. Soil temperature (at 15 cm depth) averaged across vegetation zones was higher by approximately 3 °C at Sage Lot and Fox Hill than at Round Marsh (Table 3). Soil moisture (at 5 cm depth) averaged across vegetation zone differed between the three sites, decreasing from Sage Lot to Round Marsh with Fox Hill intermediate. Significant site x vegetation zone interaction indicated that Phragmites zone soil moisture at Fox Hill was similar to Round Marsh, while native vegetation soil moisture at Fox Hill was similar to Sage Lot (Table 3). Soil organic content averaged between zones was significantly greater at Fox Hill than Round Marsh (Table 3).
Phragmites stand structure varied along the salinity gradient. Although not significant, trends in Phragmites stem height and live and dead stem counts were observed between sites (Table 3). Average Phragmites stem height displayed a trend of decrease with increasing site salinity. Live and dead Phragmites stem densities were generally greater at higher-salinity sites (Fox Hill and Sage Lot) than at Round Marsh.
GHG Fluxes
Daytime CH4 fluxes were significantly greater (by up to several orders of magnitude) in Phragmites zones than in native vegetation zones at all sites (Fig. 2) and were orders of magnitude larger for both vegetation zones at oligo-mesohaline Round Marsh than polyhaline Sage Lot (Fig. 3). CH4 emissions were highly variable and ranged from 0 to 4206 μmol m−2 h−1. They increased after the early growing season at meso-polyhaline Fox Hill (trend) and polyhaline Sage Lot (significantly); by contrast, however, oligo-mesohaline Round Marsh displayed a trend of larger CH4 emissions during the early growing season, which declined later in the growing season (Fig. 2).
Daytime CO2 fluxes ranged from −37 − +7 μmol m−2 s −1, with significantly greater uptake (by 5–15 times during the mid growing season) in the Phragmites zone than in native vegetation at Fox Hill and Sage Lot, the higher-salinity sites (Fig. 2). The greatest Phragmites zone CO2 uptake (approximately 2 × as much as at Round Marsh and Sage Lot), as well as the greatest native vegetation zone CO2 emission (positive fluxes), occurred at Fox Hill (intermediate salinity). CO2 fluxes varied across the growing season at Round Marsh and Fox Hill, with the least CO2 uptake occurring during the early growing season. For CO2 fluxes at Fox Hill, interaction of vegetation type and seasonal stage were significant, indicating that greatest CO2 uptake occurred in Phragmites zones during the mid growing season.
No detectable N2O fluxes were observed (with a 30-s averaging period and minimal detection limit of approximately 1.4 μmol m−2 h−1) (Brannon et al. in prep).
Across all sites, CH4 emissions in Phragmites zones (but not native vegetation) were negatively correlated with salinity (Spearman’s r = −0.43, p = 0.04). Soil redox potential was negatively correlated with CO2 flux magnitude in native vegetation (Spearman’s r = −0.88, p < 0.01), while soil temperature (Spearman’s r = 0.78, p = 0.01), and soil moisture (Spearman’s r = 0.72, p = 0.04) were positively correlated with CO2 flux magnitude in that zone. No other significant relationships were found between GHG fluxes and edaphic and plant variables.
Discussion
Phragmites Zones Were Consistently Associated with Larger CH4 Emissions Across the Salinity Gradient
Site histories prior to Phragmites invasions may vary. Therefore, experimental manipulations would be required in order to determine whether Phragmites invasion drove the observed consistent greater emission of CH4 compared to native vegetation zones. However, the clear association between Phragmites presence and increased daytime CH4 emissions during the growing season across sites suggests a role of this invasive species in driving GHG dynamics. These findings are consistent with Phragmites' known promotion of advective and diffusive fluxes of gases from soils to atmosphere (Armstrong et al. 1996; Brix et al. 1996; Colmer 2003), as well as potential greater C substrate provision (as a result of greater Phragmites biomass relative to native species) to methanogens in the form of rhizodeposition or litter (as reviewed in Lovell 2005).
In salt marshes, plant zonation follows strong gradients in multiple environmental conditions. Significantly larger emissions from Phragmites than from native vegetation zones at all sites along the salinity gradient may thus reflect a combination of edaphic and plant-driven factors. CH4 emissions differed between zones despite similarity in mid-growing season surface soil variables (pH, redox, temperature, moisture, and organic content), a finding that may suggest direct CH4 emission enhancement by Phragmites. However, the similarity of edaphic variables in surface soils (0–15 cm) does not rule out potential for significant differences in these and other factors between vegetation zones at depths greater than we sampled. Phragmites' ability to alter conditions in its rhizosphere environment is well-documented, with reported effects including decreased surface soil salinity (Windham and Lathrop 1999), oxygenation of the rhizosphere (Colmer 2003) and enhanced sediment accretion (Rooth et al. 2003). Given the plant’s characteristic deep (up to 1 m) root system (Brix 1987; Moore et al. 2012), it is reasonable to suspect that Phragmites rhizosphere conditions may have contributed to the observed pattern of CH4 emissions.
While this study found differences in CH4 emissions between Phragmites and native high marsh vegetation zones, an investigation comparing GHG fluxes between Phragmites and low marsh native Spartina alterniflora did not. Emery and Fulweiler (2014) measured GHG fluxes between January and September from Phragmites and S. alterniflora zones at Plum Island Estuary (mesohaline) and found that GHG fluxes (CO2, CH4 and N2O) at this site did not differ between the two vegetation zones. The authors' reported growing season CH4 fluxes were highly variable, but generally fall within the ranges of those we observed at higher-salinity sites. Their single exceptionally high S. alterniflora CH4 flux of over 18,000 μmol m−2 h−1, however, exceeds our greatest measured fluxes at any site by an order of magnitude. The discrepancy between our findings and those of Emery and Fulweiler are likely due a combination of methods differences, key ecophysiological differences in S. alterniflora and the high marsh species S. patens and D. spicata, and effects of the season during which measurements were conducted. In addition, the ability of CRDS technology to detect fluxes over much shorter periods than gas chromatograph-based methods (6–10 min vs. 60 min) may have allowed for better differentiation between vegetation zones.
In a mesocosm experiment, greater CH4 emissions were attributed to more abundant biomass of invasive relative to native Phragmites (Mozdzer and Megonigal 2013). In our study, however, metrics indicative of Phragmites biomass (stem density and height) did not correlate with CH4 emissions, suggesting that differences in subsurface soil conditions or belowground biomass may instead be responsible for observed patterns of CH4 fluxes.
Greater CO2 Uptake by Phragmites Zones may Suggest Potential for Enhanced C Sequestration
Greater CO2 uptake by Phragmites relative to native vegetation zones over the course of the growing season at Fox Hill and Sage Lot is reasonable given Phragmites' substantially greater biomass (Windham 2001) (and therefore more photosynthetic uptake) relative to smaller native high marsh species. At oligohaline Round Marsh, mid-growing season uptake by native vegetation greater than that measured at other sites could be due to greater aboveground biomass (which was not measured in this study) or to reduced salinity stress.
At Round Marsh and Sage Lot, mid-season Phragmites zone CO2 uptake is similar to that reported by Emery and Fulweiler (2014) (approximately 11 μmol m−2 s−1). Mid-growing season uptake at intermediate-salinity Fox Hill, however, averaged more than twofold greater at about 30 μmol m−2 s−1. Such difference in uptake magnitude between Fox Hill and Sage Lot is surprising since Phragmites live stem densities and heights were similar between these sites (Table 3), and may suggest an influence of soil-driven CO2 emission that counters plant-mediated uptake at Sage Lot.
Although CH4 fluxes in Phragmites zones were larger than in native vegetation zones, they were small compared to measured Phragmites zone CO2 uptake rates on a gram-to-gram C basis. Therefore, based on daytime, low tide fluxes measured during this growing season study, net CH4 emissions were not sufficient to offset net CO2 uptake. However, CO2 uptake is diminished (and emissions therefore increased) during the evening, and studies over annual or tidal cycles will likely exhibit reduced overall uptake of CO2.
Phragmites' substantial increase in daytime CO2 uptake relative to native vegetation, coupled with its known slow rates of decomposition and high productivity rates relative to S. patens (Windham 2001) and its promotion of marsh accretion (Rooth et al. 2003), may suggest that its presence could ultimately enhance marsh C sequestration. However, such a conclusion must be based on more detailed temporal GHG flux measurements (seasonal and diel), including longer term gas ebullition studies, and coupled with measurements of long-term C sequestration rates.
Comparing GHG Emissions Across Seasonal Stages and Complex Environmental Gradients
Since Phragmites commonly invades marshes from the landward edge (Amsberry et al. 2000) and therefore displaces native high marsh species, GHG fluxes need to be characterized in order to assess ecosystem-scale response to a changing vegetation community. This study broadens understanding of growing season patterns of daytime CO2 and CH4 fluxes across the complex marsh landscape and over a growing season period.
Phragmites zones exhibited distinct temporal CH4 flux trends along the salinity gradient, with fluxes increasing over the course of the growing season in the meso-polyhaline sites and decreasing at the oligohaline site. The observed increase in net CH4 emissions from early to late seasonal stages in the more saline sites may imply a role of plant-mediated transport and/or an increase as the growing season progresses in microbial CH4 production that is not being offset by increased microbial CH4 oxidation. At the oligohaline site, observed temporal patterns of CH4 fluxes may imply that vegetation presence decreases CH4 emissions (potentially by soil oxygenation) and/or that microbial CH4 production decreases as the growing season progresses.
The difference in CH4 emissions between sites is consistent with the known control of salinity on marsh CH4 emission (Bartlett et al. 1987; Mitsch and Gosselink 2000; Poffenbarger et al. 2011; Madigan 2012), but other variables (soil moisture, redox potential, and pore water sulfide concentration) also vary along the salinity gradient. CH4 emissions were greatest at Round Marsh, the site of lowest soil salinity, moisture and porewater sulfide and least reduced conditions, and smallest at Sage Lot, which was characterized by greatest soil salinity, moisture and sulfide concentrations and most reduced conditions. Fox Hill’s soil conditions were intermediate. These findings support known roles of salinity and sulfate availability as strong predictors of marsh CH4 emission magnitude, but contradict known positive relationships between methanogenesis and anaerobic, reduced soil conditions. Given the difficulty in determining relative contributions of soil variable and plant-mediated effects on GHG fluxes, future research should be directed toward experimentation to discern biotic and abiotic feedbacks along these environmental gradients.
Conclusions
Phragmites-dominated zones were characterized by significantly larger daytime CH4 emissions than native high marsh vegetation zones along the natural salinity gradient, and larger daytime CO2 uptake rates were observed in Phragmites zones in meso-polyhaline marshes. Although this study is not able to discern relative impacts of physical and biological controls on observed CO2 and CH4 fluxes, it reveals differences between two marsh zones for which GHG fluxes had not previously been compared and therefore confirms a need for future manipulative experiments to test mechanisms driving flux differences. In order to determine whether Phragmites may affect marsh net GHG uptake and C sequestration in the long term, future studies should monitor GHG fluxes over annual and diel cycles and investigate how rates of Phragmites-zone C sequestration compare with rates in native vegetation zones.
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Acknowledgments
This work was supported by the USDA National Institute of Food and Agriculture (Hatch project # 229286, grant to Moseman-Valtierra) and the National Science Foundation EPSCoR Coperative Agreement (#EPS-1004057, fellowship to Martin). Waquoit Bay National Estuarine Research Reserve provided access to our Sage Lot Pond site. C. Wigand provided valuable feedback on this manuscript, and we sincerely thank her for her helpful comments. We thank I. Armitstead, L. Brannon, I. China, S. Doman, S. Kelley, T. Moebus, A. Moen, and R. Quinn for field support, and C. Martin for assistance with development of R code for expediting data analysis. We are grateful to three anonymous reviewers for their helpful comments, which greatly improved the quality of this manuscript.
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Martin, R.M., Moseman-Valtierra, S. Greenhouse Gas Fluxes Vary Between Phragmites Australis and Native Vegetation Zones in Coastal Wetlands Along a Salinity Gradient. Wetlands 35, 1021–1031 (2015). https://doi.org/10.1007/s13157-015-0690-y
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DOI: https://doi.org/10.1007/s13157-015-0690-y