Abstract
The edible freshwater fishes of several genera including Labeo, Cyprinus, Cirrhinus, Danio, Puntius, Garra (Cypriniformes), Channa (Channiformes), Clarias, Heteropneustes, Rita, Monopterus, Ompak, Bagarius and Mystus (Siluriformes) in Meghalaya State were examined for their helminth parasite spectrum. Two monogenean flukes representing the genera Diplozoon and Bifurcohaptor were recovered from the gills of the host fish, which are redescribed herein and their descriptions supplemented with information on their surface fine topography. Labeo pangusia and L. boga constitute new host records for the diplozoid monogenean. Both the monogenean species are reported for the first time from the fishes in Meghalaya, a new locality record.
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Introduction
The spectrum of helminth parasites of freshwater piscine hosts has been scantily studied and there is limited information available on this aspect pertaining to the northeastern region of India. In context of Meghalaya there are very few reports available on the parasitic helminth fauna of fishes, wherein the occurrence of some trematode species has been recorded (Srivastava and Ghosh 1967; Soota and Ghosh 1977); however the caryophyllidean cestode fauna of siluriform fishes is relatively well documented (Chakravarty and Tandon 1988; Tandon et al. 2005).
During an exploratory survey of edible freshwater fishes in Meghalaya, a wide spectrum of Cypriniformes, Channiformes and Siluriformes fish species were examined for the platyhelminth fauna sustained by them. Of the fishes examined, the species of Labeo (Cypriniformes) and Mystus (Siluriformes) were found to harbor two monogenean fluke species. In the present communication we redescribe these species, the occurrence of which is being reported for the first time from the northeastern region of India.
Materials and methods
A large number of specimens of Bifurcohaptor indicus were recovered from the gills of the catfish Mystus tengara and M. vittatus and 45 specimens of Diplozoon cauveri were collected from the gill filaments of 42 Labeo pangusia and 8 specimens from 6 Labeo boga. The recovered parasites were flattened, fixed in 70% alcohol and processed for wholemount preparations following standard procedure and using Borax carmine and Mayer’s carmalum as stains. For scanning electron microscopy (SEM) the specimens fixed in 4% cold neutral buffered formalin were processed and treated with tetramethylsilane (Roy and Tandon, 1991), metal coated and viewed under a LEO 435 VP scanning electron microscope at accelerating electron voltage ranging between 10–20 kV. Measurements of the body and the various structures are given in millimeters.
Results
Monopisthocotylea
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Order: Dactylogyridea Bychowsky, 1937
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Family: Dactylogyridae Bychowsky, 1933
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Genus: Bifurcohaptor Jain, 1958
Body elongated, blunt anteriorly. Cephalic region broad, head organs paired, with a set of four organs lying on either lateral side of anterior end. Eye spots two pairs, posterior pair larger than anterior. Opisthaptor forceps-like, deeply bifurcated, nearly 1/3rd of total body length; armature of opisthaptor comprising two pairs of anchors, larger dorsal and smaller ventral; each dorsal anchor with three sclerotized plates—one median, two dorsolateral, anchor comprising a stout base, long shaft and strong recurved points; ventral anchors very small, lying at extremities of haptor, each with bifurcate base, shaft and fine recurved point, ventral bars two. Pharynx oval; oesophagus short; intestinal ceaca bifurcated, united just anterior to haptor. Testis and ovary single; male copulatory complex consisting of copulatory tube and single accessory piece. Vitellaria follicular, densely spread throughout body except at region of reproductive organs. Eggs not observed. SEM observations revealed the presence of smooth body surface, devoid of any spines or papillate structures. Measurements of the body are provided in Table 1.
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Host—Mystus tengara, M. vittatus
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Location—Gill filaments
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Locality—Dawki, Byrnihat (Meghalaya).
Remarks
The genus Bifurcohaptor was erected by Jain (1958) with B. indicus as its type species, from gill filaments of Mystus vittatus at Lucknow. The genus Bagaritrema, described by Tripathi (1959) from gill filament of Bagarius bagarius, is a synonym of Bifurcohaptor (Yamaguti, 1963). Species of Bifurcohaptor so far reported from India include: B. indicus Jain, 1958 from Mystus vittatus; B. giganticus Jain, 1958 (= Bagaritrema son Tripathi, 1959) from M. seengala (= Sperata seengala); B. minutum Kulkarni, 1969 from M. tengara; B. vishwanathai Agarwal and Kumar, 1977 from Bagarius bagarius; B. mulleri Gupta and Sharma, 1981 from B. bagarius; B. tripathii Gupta and Sharma, 1981 from Channa striatus; B. gorakhnathai Kumar and Agarwal, 1982 from B. bagarius; B. sohani Agarwal and Singh, 1982 from M. vittatus; B. hemlatae Gupta, 1984 from Rita rita; B. ramalingami Swarup and Jain, 1984 from M. vittatus and B. bagarius; B. kulkarnii Swarup and Jain, 1984 from B. bagarius; B. chauhani Agarwal and Sharma, 1986 from B. bagarius; and B. pedunculata Pandey, Agarwal and Tripathi, 2002 from M. vittatus.
Pandey and Singh (1989) studied the validity of Indian species of Bifurcohaptor and regarded only B. indicus as a valid species, with all others being its synonyms. However, Dubey et al. (1990) regarded B. giganticus, B. son, B. tripathii and B. hemlatae to be valid species, whereas Lim et al. (2001) retained only B. indicus, B. giganticus and B. son as valid species and considered the rest as species inquirendae. Pandey et al. (2002) concluded that catfishes of the Indian sub-continent harbor only two species viz. B. indicus and B. giganticus.
The present description of B. indicus tallies with the original description of the species, with minor modifications in form and measurements of the body structures. B. indicus has been earlier reported from Mystus tengara, M. keletius and M. nemurus (= Hemibagrus nemurus) besides its type host. Dawki and Byrnihat, (Meghalaya), as reported herein, are new locality records for this species.
Polyopisthocotylea
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Order: Mazocraeidea Bychowsky, 1957
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Family: Diplozoidae Tripathi, 1957
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Genus: Diplozoon Nordmann, 1832
Flukes always occurring in permanent pairing, two flukes joined in posterior half of body, in shape of ‘X’; body pale white in colour, forebody longer than hindbody. Prohaptor comprising two cup-shaped suckers. Opisthaptor with four pairs of lateral clamps, rectangular in shape and concave ventrally. Mouth situated on ventral side of anterior extremity; pharynx just behind prohaptoral region. Gut single, intestine not bifurcate but with numerous lateral diverticula. Reproductive organs located in anterior part of hindbody. Testis single, spherical, slightly lobed. Ovary pretesticular. Vitelline follicles extensive, scattered in whole forebody. In utero eggs one, occasionally two in number, oval, without polar filaments. SEM observation showed the presence of rough body surface with absence of spines or papillae. Measurements of the fluke are provided in Table 2.
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Host—Labeo pangusia, L. boga
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Location—Gill filaments
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Locality—Dawki, Shella, Sonapur (Meghalaya).
Remarks
The genus Diplozoon, with type species D. paradoxum Nordmann, 1832, was first described from the gills of Abramis bramis brama. The species of Diplozoon reported from India so far are: D. indicum Dayal, 1941 from Barbus sarana; D. kashmirensis Kaw, 1950 from Schizothorax niger and S. esocinus; D. soni Tripathi, 1957 from Oxygaster bacaila; D. cauveri Tripathi, 1959 from Cirrhina cirrhosa; D. microclampi Kulkarni, 1971 from Barbus sarana; D. thapari Gupta and Krishna, 1979 from Tor tor and D. dasashwamedhai Agarwal and Kumar, 1989 from Barilius bola.
Fotedar and Parveen (1987) also recorded D. nipponicum Goto, 1891 from Cyprinus carpio specularis from Kashmir. However, Pandey et al. (2002) regarded D. nipponicum Goto, 1891 as a synonym of D. kashmirensis. Further, Pandey and Agarwal (2008) considered D. indicum and D. nipponicum as the only valid species.
The presence of eggs with a long coiled filament is a diagonistic characteristic of the genus Diplozoon. However, D. cauveri was described as having eggs without polar filament (Tripathi, 1959). The present observations of the diplozoid fluke under study tally with the original description of D. cauveri in having non filamented eggs. In view of this character, i.e., eggs without polar filaments, D. cauveri should be recognized as a valid species of the genus. As reported herein, Labeo pangusia and L. boga are new host records and Meghalaya, a new locality record for D. cauveri.
References
Agarwal GP, Kumar R (1977) On a new monogenetic trematode, Bifurcohaptor vishwanathi n.sp. from the gills of a freshwater fish, Bagarius bagarius (Ham.). J Zool Soc India 29:73–79
Agarwal GP, Kumar (1989) On a new monogenetic trematode, Diplozoon dasashwamedhai n.sp. from the gills of freshwater fish, Barilius bola (Ham.). Indian J Helminthol 41:148–151
Agarwal N, Sharma R (1986) Bifurcohaptor chauhani n.sp. (Monogenea) from a freshwater fish Bagarius bagarius (Ham.) caught at Lucknow. Indian J Helminthol (n.s.) 3:37–42
Agarwal N, Singh HS (1982) Two freshwater monogeneans of the genus Bifurcohaptor Jain, 1958 and Dactylogyrus Diesing, 1850. Indian J Parasitol 6:215–218
Chakravarty R, Tandon V (1988) On the present status of caryophyllidea with a report on some caryophyllid infections in the freshwater catfish, Clarias batrachus (L) in Northeast India and a record of an anomalous form. Indian J Helminthol (n.s.) 5:37–54
Dayal J (1941) On a new trematode. Diplozoon indicum n.sp., from a freshwater fish Barbus (Puntius) sarana (Ham.). Proc Natl Acad Sci India 11:93–96
Dubey A, Gupta AK, Agarwal SM (1990) Studies on monogenea parasites in freshwater fishes at Raipur. VI. A review, synonymy of species of the genus Bifurcohaptor Jain, 1958. Ancylodiscoidinae: Ancyrocephalidae. Indian J Helminthol 42:152–163
Fotedar DN, Parveen QN (1987) On two species of trematode genus Diplozoon Nordmann 1832, from fishes of Kashmir and notes on some species of the genus. Indian J Helminthol 39:66–76
Goto S (1891) On Diplozoon nipponicum n.sp. J Coll Sci Imp Univ Tokyo 4:151–192
Gupta PC (1984) Bifurcohaptor hemlatae n. sp. (Monogenea: Dactylogyridae) from a freshwater fish Rita rita from Kanpur. Indian J Parasitol 7:233–235
Gupta SP, Krishna (1979) Monogenetic trematodes of fishes. On a new monogenetic trematode Diplozoon thapari n. sp. from gill filaments of hill stream fish, Tor tor (Ham.) from Nanak Sagar Dam. Nainital. Indian J Helminthol 29:137–139
Gupta SP, Sharma RK (1981) Two new monogenetic trematodes of the genus Bifurcohaptor Jain, 1958 from freshwater fishes of Uttar Pradesh. Indian J Helminthol 33:38–44
Jain SL (1958) Monogenea of Indian freshwater fishes. VII. Bifurcohaptor, a new genus of freshwater Tetraonchinae from the gill filament of two fishes from Lucknow. J Parasitol 44:388–394
Kaw BL (1950) Studies in helminthology: helminth parasites of Kashmir. Part I. Trematoda. Indian J Helminthol 2:67–69
Kulkarni T (1969) Studies on the monogenetic trematodes of fishes found in Hyderabad, Andhra Pradesh (India). Part I. Riv Parassitol 30:73–90
Kulkarni T (1971) Studies on the monogenetic trematodes of fishes found in Hyderabad (India). Diplozoon microclampi n.sp. from the gills of Barbus sarana. Zool Anz 186:379–381
Kumar R, Agarwal GP (1982) On a new Bifurcohaptor species (Monogenea) from Bagarius bagarius (Pisces) in India. Angew Parasitol 23:135–137
Lim LHS, Timofeeva TA, Gibson DI (2001) Dactylogyridean monogeneans of the siluriform fishes of the Old World. Syst Parasitol 50:159–197
Nordmann A (1832) Mikrographische Beitrage zur Naturgeschichto der Wirbellosen Thiere. Berlin. 1 Heft: X + 118; Heft: XVIII + 150
Pandey KC, Agarwal N (2008) An encyclopaedia of Indian monogenoidea. Vitasta Publishing, New Delhi
Pandey KC, Singh HS (1989) A review of the genus Bifurcohaptor Jain, 1958. Indian J Helminthol (n.s.) 6:39–56
Pandey KC, Agarwal N, Tripathi A (2002) Remarks on Indian species of Dactylogyroides Gusev, 1976, with description of a new species on freshwater cyprinids of Lucknow. Indian J Helminthol (n.s.) 20:15–28
Roy B, Tandon V (1991) Usefulness of tetramethylsilane in the preparation of helminth parasites for scanning electron microscopy. Riv Parassitol 8:207–215
Soota TD, Ghosh RK (1977) On some trematodes from Meghalaya. Record Zool Surv India 73:111–122
Srivastava CB, Ghosh RK (1967) On a new genus Paramacrolecithus (Trematoda: Allocreadiidae) from a cyprinid fish, Rasbora rasbora (Ham.). Proc Indian Acad Sci 66:179–183
Swarup M, Jain SP (1984) On three new species of the genus Bifurcohaptor Jain, 1958 (Monogenea: Dactylogyridae) with remarks on pathological effects. Indian J Helminthol (n.s.) 1 & 2:106–110
Tandon V, Chakravarty R, Das B (2005) Four new species of the genus Lytocestus (Caryophyllidea, Lytocestidae) from edible catfishes in Assam and Meghalaya, India. J Parasit Dis 29:131–142
Tripathi YR (1957) Monogenetic trematodes from fishes of India. Indian J Helminthol 9:1–149
Tripathi YR (1959) Monogenetic trematodes from fishes of India. Indian J Helminthol 9:1–149
Yamaguti S (1963) Systema Helminthum. IV. Monogenea and Aspidocotylea. Interscience Publishers, New York
Acknowledgments
The present study was supported by the All India Co-ordinated Project on Capacity Building in Taxonomy: Research on Helminths (AICOPTAX; MoE&F, GOI) to VT and DSA (UGC-SAP) Programme in Zoology, NEHU.
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Thapa, S., Jyrwa, D.B. & Tandon, V. A new report on the occurrence of monogenean parasites (Monogenoidea) on gill filaments of freshwater fishes in Meghalaya. J Parasit Dis 35, 80–84 (2011). https://doi.org/10.1007/s12639-011-0023-x
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DOI: https://doi.org/10.1007/s12639-011-0023-x