Abstract
The simultaneous removal of phenol and selenite from synthetic wastewater was investigated by adopting two different co-culturing techniques using the fungus Phanerochaete chrysosporium and the bacterium Delftia lacustris. Separately grown biomass of the fungus and the bacterium (suspended co-culture) was incubated with different concentrations of phenol (0–1,200 mg/L) and selenite (10 mg/L). The selenite ions were biologically reduced to extracellular Se(0) nanoparticles (3.58 nm diameter) with the simultaneous degradation of up to 800 mg/L of phenol. Upon growing the fungus and the bacterium together using an attached growth co-culture, the bacterium grew as a biofilm onto the fungus. The extracellularly produced Se(0) in the attached growth co-culture had a minimum diameter of 58.5 nm. This co-culture was able to degrade completely 50 mg/L phenol, but was completely inhibited at a phenol concentration of 200 mg/L.
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Acknowledgements
The authors would like to thank Prof. Debabrata Chakraborty and Dr. Arup Mandal (Department of Polymer Science and Technology, University of Calcutta, India) for their support with the FT-IR, SEM and TEM analysis. This work was supported by the Marie Sklodowska-Curie European Joint Doctorate (EJD) in Advanced Biological Waste-To-Energy Technologies (ABWET) funded from Horizon 2020 [grant number 643071].
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Competing financial interests: S.C. declares no competing financial interests. E.R.R. declares no competing financial interests. P.N.L.L. declares no competing financial interests.
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Chakraborty, S., Rene, E.R. & Lens, P.N.L. Reduction of selenite to elemental Se(0) with simultaneous degradation of phenol by co-cultures of Phanerochaete chrysosporium and Delftia lacustris. J Microbiol. 57, 738–747 (2019). https://doi.org/10.1007/s12275-019-9042-6
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DOI: https://doi.org/10.1007/s12275-019-9042-6