Abstract
We previously reported the development of a neutralization assay system for evaluating Japanese Encephalitis Virus (JEV) neutralizing antibody (NAb) using pseudotyped-JEV (JEV-PV). JEV-PV-based neutralization assay offers several advantages compared with the current standard plaque-reduction neutralization test (PRNT), including simplicity, safety, and speed. To evaluate the suitability of the JEV-PV assay as new replacement neutralization assay, we compared its repeatability, reproducibility, specificity, and correlated its results with those obtained using the PRNT. These analyses showed a close correlation between the results obtained with the JEV-PV assay and the PRNT, using the 50% plaque reduction method as a standard for measuring NAb titers to JEV. The validation results met all analytical acceptance criteria. These results suggest that the JEV-PV assay could serve as a safe and simple method for measuring NAb titer against JEV and could be used as an alternative approach for assaying the potency of JEV neutralization.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Abe, M., Kuzuhara, S., and Kino, Y. 2003. Establishment of an analyzing method for a Japanese encephalitis virus neutralization test in Vero cells. Vaccine 21, 1989–1994.
Ahsan, M.F. and Gore, M.M. 2011. Comparison of immune response generated against Japanese encephalitis virus envelope protein expressed by DNA vaccines under macrophage associated versus ubiquitous expression promoters. Virology J. 8, 382.
Biswas, S.M., Ayachit, V.M., Sapkal, G.N., Mahamuni, S.A., and Gore, M.M. 2009. Japanese encephalitis virus produces a CD4+ Th2 response and associated immunoprotection in an adoptive-transfer murine model. J. Gen. Virol. 90, 818–826.
Elias, C., Okwo-Bele, J.M., and Fischer, M. 2009. A strategic plan for Japanese encephalitis control by 2015. Lancet Infect. Dis. 9, 7.
Fischer, M., Lindsey, N., Staples, J.E., and Hills, S. 2010. Japanese encephalitis vaccines: recommendations of the Advisory Committee on Immunization Practices (ACIP). MMWR Recomm. Rep. 59, 1–27.
Halstead, S.B. and Thomas, S.J. 2010. Japanese encephalitis: new options for active immunization. Clin. Infect. Dis. 50, 1155–1164.
Hombach, J., Solomon, T., Kurane, I., Jacobson, J., and Wood, D. 2005. Report on a WHO consultation on immunological endpoints for evaluation of new Japanese encephalitis vaccines, WHO, Geneva, 2–3 September, 2004. Vaccine 23, 5205–5211.
Jmor, F., Emsley, H.C., Fischer, M., Solomon, T., and Lewthwaite, P. 2008. The incidence of acute encephalitis syndrome in Western industrialised and tropical countries. Virol. J. 5, 134.
Johnson, B.W., Kosoy, O., Hunsperger, E., Beltran, M., Delorey, M., Guirakhoo, F., and Monath, T. 2009. Evaluation of chimeric Japanese encephalitis and dengue viruses for use in diagnostic plaque reduction neutralization tests. Clin. Vaccine Immunol. 16, 1052–1059.
Kim, Y.B., Lee, M.K., Han, D.P., and Cho, M.W. 2001. Development of a safe and rapid neutralization assay using murine leukemia virus pseudotyped with HIV type 1 envelope glycoprotein lacking the cytoplasmic domain. AIDS Res. Hum. Retroviruses 17, 1715–1724.
Konishi, E., Shoda, M., Ajiro, N., and Kondo, T. 2004. Development and evaluation of an enzyme-linked immunosorbent assay for quantifying antibodies to Japanese encephalitis virus nonstructural 1 protein to detect subclinical infections in vaccinated horses. J. Clin. Microbiol. 42, 5087–5093.
Lee, M.S., Cohen, B., Hand, J., and Nokes, D.J. 1999. A simplified and standardized neutralization enzyme immunoassay for the quantification of measles neutralizing antibody. J. Virol. Methods 78, 209–217.
Lee, H.J., Min, K.I., Lee, J., Kang, S.H., Jeon, W., Nam, J.H., Ju, Y.R., and Kim, Y.B. 2009. The prM-independent packaging of pseudotyped Japanese encephalitis virus. Virol. J. 6, 115.
Lobigs, M., Pavy, M., Hall, R.A., Lobigs, P., Cooper, P., Komiya, T., Toriniwa, H., and Petrovsky, N. 2010. An inactivated Vero cell-grown Japanese encephalitis vaccine formulated with Advax, a novel inulin-based adjuvant, induces protective neutralizing antibody against homologous and heterologous flaviviruses. J. Gen. Virol. 91, 1407–1417.
Markoff, L. 2000. Points to consider in the development of a surrogate for efficacy of novel Japanese encephalitis virus vaccines. Vaccine 18 Suppl 2, 26–32.
Martin, J.E., Louder, M.K., Holman, L.A., Gordon, I.J., Enama, M.E., Larkin, B.D., Andrews, C.A., Vogel, L., Koup, R.A., Roederer, M., and et al. 2008. A SARS DNA vaccine induces neutralizing antibody and cellular immune responses in healthy adults in a Phase I clinical trial. Vaccine 26, 6338–6343.
Pierson, T.C., Sanchez, M.D., Puffer, B.A., Ahmed, A.A., Geiss, B.J., Valentine, L.E., Altamura, L.A., Diamond, M.S., and Doms, R.W. 2006. A rapid and quantitative assay for measuring antibody-mediated neutralization of West Nile virus infection. Virology 346, 53–65.
Roehrig, J.T., Hombach, J., and Barrett, A.D. 2008. Guidelines for plaque-reduction neutralization testing of human antibodies to dengue viruses. Viral Immunol. 21, 123–132.
Schnierle, B.S., Stitz, J., Bosch, V., Nocken, F., Merget-Millitzer, H., Engelstadter, M., Kurth, R., Groner, B., and Cichutek, K. 1997. Pseudotyping of murine leukemia virus with the envelope glycoproteins of HIV generates a retroviral vector with specificity of infection for CD4-expressing cells. Proc. Natl. Acad. Sci. USA 94, 8640–8645.
Shyu, W.R., Wang, Y.C., Chin, C., and Chen, W.J. 1997. Assessment of neutralizing antibodies elicited by a vaccine (Nakayama) strain of Japanese encephalitis virus in Taiwan. Epidemiol. Infect. 119, 79–83.
Simoes, M., Camacho, L.A., Yamamura, A.M., Miranda, E.H., Cajaraville, A.C., and da Silva Freire, M. 2012. Evaluation of accuracy and reliability of the plaque reduction neutralization test (micro-PRNT) in detection of yellow fever virus antibodies. Biologicals 40, 399–404.
Solomon, T. 2003. Recent advances in Japanese encephalitis. J. Neurovirol. 9, 274–283.
Solomon, T., Dung, N.M., Kneen, R., Gainsborough, M., Vaughn, D.W., and Khanh, V.T. 2000. Japanese encephalitis. J. Neurol. Neurosurg. Psychiatry 68, 405–415.
Ting, S.H., See, E., Tan, H.C., Lee, M.A., and Ooi, E.E. 2001. Development of a simplified assay for the detection of neutralizing antibodies to Japanese encephalitis virus. J. Virol. Methods 93, 43–47.
Yang, D.K., Kim, B.H., Lim, S.I., Kwon, J.H., Lee, K.W., Choi, C.U., and Kweon, C.H. 2006. Development and evaluation of indirect ELISA for the detection of antibodies against Japanese encephalitis virus in swine. J. Vet. Sci. 7, 271–275.
Author information
Authors and Affiliations
Corresponding author
Additional information
These authors contributed equally to this work Copyright 2014, The Microbiological Society of Korea
Rights and permissions
About this article
Cite this article
Lee, HJ., Min, KI., Park, K.H. et al. Comparison of JEV neutralization assay using pseudotyped JEV with the conventional plaque-reduction neutralization test. J Microbiol. 52, 435–440 (2014). https://doi.org/10.1007/s12275-014-3529-y
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12275-014-3529-y