Abstract
A study on the effects of light intensity (40 and 80 μE/m2/sec) on the components and topographical structures of extracellular polysaccharides (EPS) was carried out in cyanobacteria Nostoc sp.. EPS yield increased with light intensity. However, light intensity did not significantly affect the EPS fractions and monosaccharide composition. Higher light intensity generally resulted in higher protein content of EPS in similar fractions. The topographical structure of EPS, investigated by atomic force microscopy, appeared as spherical lumps, chains and networks. The long chains were observed at higher light intensity. Thus, light intensity affected the yield and nature of EPS.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Bellezza, S., Paradossi, G., De Philippis, R., and Albertano, P. 2003. Leptolyngbya strains from Roman hypogea: cytochemical and physico-chemical characterisation of exopolysaccharides. J. Appl. Phycol. 15, 193–200.
Chen, L., Li, D., and Liu, Y. 2003. Salt tolerance of Microcoleus vaginatus Gom., a cyanobacterium isolated from desert algal crust, was enhanced by exogenous carbohydrates. J. Arid Environ. 55, 645–656.
Chen, L., Wang, G., Hong, S., Liu, A., Li, C., and Liu, Y. 2009. UV-B-induced oxidative damage and protective role of exopolysaccharides in desert cyanobacterium Microcoleus vaginatus. J. Integr. Plant Biol. 51, 194–200.
De Philippis, R., Colica, G., and Micheletti, E. 2011. Exopolysaccharide-producing cyanobacteria in heavy metal removal from water: molecular basis and practical applicability of the biosorption process. Appl. Microbiol. Biotechnol. 92, 697–708.
De Philippis, R., Sili, C., Paperi, R., and Vincenzini, M. 2001. Exopolysaccharides-producing cyanobacteria and their possible exploitation: a review. J. Appl. Phycol. 13, 293–299.
De Philippis, R. and Vincenzini, M. 1998. Exocellular polysaccharides from cyanobacteria and their possible applications. FEMS Microbiol. Rev. 22, 151–175.
De Philippis, R. and Vincenzini, M. 2003. Outermost polysaccharidic investments of cyanobacteria: nature, significance and possible applications. Recent Res. dev. Microbiol. 7, 13–22.
Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., and Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350–356.
Ehling-Schulz, M., Bilger, W., and Scherer, S. 1997. UV-B-induced synthesis of photoprotective pigments and extracellular polysaccharides in the terrestrial cyanobacterium Nostoc commune. J. Bacteriol. 179, 1940–1945.
Friedman, O., Dubinsky, Z., and Arad, S. 1991. Effect of light intensity on growth and polysaccharides production in red and blue-green rhodophyta unicells. Bioresour. Technol. 38, 105–110.
Hokputsa, S., Hu, C., Paulsen, B.S., and Harding, S.E. 2003. A physico-chemical comparative study on extracellular carbohydrate polymers from five desert algae. Carbohyd. Polym. 54, 27–32.
Hu, C., Liu, Y., Paulsen, B.S., Petersen, D., and Klaveness, D. 2003. Extracellular carbohydrate polymers from five desert soil algae with different cohesion in the stabilization of fine sand grain. Carbohyd. Polym. 54, 33–42.
Hu, C., Liu, Y., Zhang, D., Huang, Z., and Paulsen, B.S. 2002. Cementing mechanism of algal crusts from desert area. Chinese Sci. Bull. 47, 1361–1368.
Huang, Z., Liu, Y., Paulsen, B.S., and Klaveness, D. 1998. Studies on polysaccharides from three edible species of Nostoc (cyanobacteria) with different colony morphologies: comparison of monosaccharide compositions and viscosities of polysaccharides from field colonies and suspension cultures. J. Phycol. 34, 962–968.
Li, P., Liu, Z., and Xu, R. 2001. Chemical characterisation of the released polysaccharides from the cyanobacterium Aphanothece halophytica GR02. J. Appl. Phycol. 13, 71–77.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275.
Mandal, S., Singh, R., and Patel, V. 2011. Isolation and characterization of exopolysaccharides secreted by a toxic dinoflagellate, Amphidinium carterae Hulburt 1957 and its probable role in harmful algal blooms (HABs). Microb. Ecol. 62, 518–527.
Moreno, J., Vargas, M.A., Olivares, H., Rivas, J., and Guerrero, M.G. 1998. Exopolysaccharides production by the cyanobacterium Anabaena sp. ATCC 33047 in batch and continuous culture. J. Biotechnol. 60, 175–182.
Mota, R., Guimaraes, R., Büttel, Z., Rossi, F., Colica, G., Silva, C.J., Santos, C., Gales, L., Zille, A., De Philippis, R., Pereira, S.B., and et al. 2013. Production and characterization of extracellular carbohydrate polymer from Cyanothece sp. CCY 0110. Carbohyd. Polym. 92, 1408–1415.
Neu, T.R. and Marshall, K.C. 1990. Bacterial polymers: physicochemical aspects of their interactions at interfaces. J. Biomater. Appl. 5, 107–133.
Otero, A. and Vincenzini, M. 2003. Extracellular polysaccharides synthesis by Nostoc strains as affected by N source and light intensity. J. Biotechnol. 102, 143–152.
Pereira, S., Zille, A., Micheletti, E., Moradas-Ferreira, P., De Philippis, R., and Tamagnini, P. 2009. Complexity of cyanobacterial exopolysaccharides: composition, structures, inducing factors and putative genes involved in their biosynthesis and assembly. FEMS Microbiol. Rev. 33, 917–941.
Pletikapic, G., Radic, T.M., Zimmermann, A.H., Svetlicic, V., Pfannkuchen, M., Maric, D., Godrijan, J., and Zutic, V. 2011. AFM imaging of extracellular polymer release by marine diatom Cylindrotheca closterium (Ehrenberg) Reiman & J.C. Lewin. J. Mol. Recognit. 24, 436–445.
Rippka, R., Deruelles, J., Waterbury, J.B., Herdman, M., and Stanier, R.Y. 1979. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol. 111, 1–61.
Su, J., Jia, S., Chen, X., and Yu, H. 2008. Morphology, cell growth, and polysaccharides production of Nostoc flagelliforme in liquid suspension culture at different agitation rates. J. Appl. Phycol. 20, 213–217.
Suresh Kumar, A., Mody, K., and Jha, B. 2007. Bacterial exopolysaccharides-a perception. J. Basic Microb. 47, 103–117.
Trabelsi, L., Ben Ouada, H., Bacha, H., and Ghoul, M. 2009. Combined effect of temperature and light intensity on growth and extracellular polymeric substance production by the cyanobacterium Arthrospira platensis. J. Appl. Phycol. 21, 405–412.
Vincenzini, M., Philippis, R., Sili, C., and Materassi, R. 1993. Stability of molecular and rheological properties of the exopolyzsaccharide produced by Cyanospira capsulata cultivated under different growth conditions. J. Appl. Phycol. 5, 539–541.
Yoshimura, H., Kotake, T., Aohara, T., Tsumuraya, Y., Ikeuchi, M., and Ohmori, M. 2012. The role of extracellular polysaccharides produced by the terrestrial cyanobacterium Nostoc sp. strain HK-01 in NaCl tolerance. J. Appl. Phycol. 24, 237–243.
Yu, H., Jia, S., and Dai, Y. 2010. Accumulation of exopolysaccharides in liquid suspension culture of Nostoc flagelliforme cells. Appl. Biochem. Biotechnol. 160, 552–560.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ge, H., Xia, L., Zhou, X. et al. Effects of light intensity on components and topographical structures of extracellular polysaccharides from the cyanobacteria Nostoc sp.. J Microbiol. 52, 179–183 (2014). https://doi.org/10.1007/s12275-014-2720-5
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12275-014-2720-5