Abstract
The objective of this study was to evaluate the survival of cowpea during bacterial colonization and evaluate the interrelationship of the Bradyrhizobium sp. and plant growth-promoting bacteria (PGPB) as a potential method for optimizing symbiotic performance and cowpea development. Two experiments using the model legume cowpea cv. “IPA 206” were conducted. In the first experiment, cowpea seeds were disinfected, germinated and transferred to sterilized Gibson tubes containing a nitrogen-free nutritive solution. The experimental design was randomized blocks with 24 treatments [Bradyrhizobium sp. (BR 3267); 22 PGPB; absolute control (AC)] with three replicates. In the second experiment, seeds were disinfected, inoculated according to their specific treatment and grown in Leonard jars containing washed and autoclaved sand. The experimental design was randomized blocks with 24 treatments [BR 3267; 22 BR 3267 + PGPB; AC] with three replicates. Scanning electron microscopy demonstrated satisfactory colonization of the roots of inoculated plants. Additionally, synergism between BR 3267 and PGPB in cowpeas was observed, particularly in the BR 3267 + Paenibacillus graminis (MC 04.21) and BR 3267 + P. durus (C 04.50), which showed greater symbiotic performance and promotion of cowpea development.
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Almeida, A.L.G., Alcantara, R.M.C.M., Nóbrega, R.S.A., Nóbrega, J.C.A., Leite, L.F.C., and Silva, J.A.L. 2010. Produtividade do feijão-caupi cv. BR 17 Gurguéia inoculado com bactérias diazotróficas simbióticas no Piauí. Agraria 5, 364–369.
Antolín, M., Fiasconaro, M.L., and Sánchez-Díaz, M. 2010. Relationship between photosynthetic capacity, nitrogen assimilation and nodule metabolism in alfalfa (Medicago sativa) grown with sewage sludge. J. Hazard Mater. 182, 210–216.
Araújo, F.F. 2008. Rizobactérias e indução de resistência a doenças em plantas. Parte II — Microrganismos Promotores de Crescimento em Plantas, pp. 197–210. In Figueiredo, M.V.B., Burity, H.A., Stamford, N.P., and Santos, C.E.R.S. (eds.), Microrganismos e Agrobiodiversidade: O novo desafio para agricultura, Agrolivros, Guaíba.
Belimov, A.A., Dodd, I.C., Hontzeas, N., Theobald, J., Safronova, V.I., and Davies, W. 2009. Rhizosphere bacteria containing 1-aminocyclopropane-1-carboxylate deaminase increase yield of plants grown in drying soil via both local and systemic hormone signaling. New Phytol. 181, 413–423.
Bengough, A.G., Bransby, M.F., Hans, J., McKenna, S.J., Roberts, T.J., and Valentine, T.A. 2006. Root responses to soil physical conditions: growth dynamics from field to cell. J. Exp. Bot. 57, 437–447.
Benincasa, M.M.P. 2003. Análise de crescimento de plantas. FUNEP, Jaboticabal.
Bremner, J.M. 1965. Total nitrogen. pp. 1149–1178. In Black, C.A. (ed.), Methods of soil analysis chemical and microbiological properties. American Society of Agronomy, Madison, USA.
Chianu, J.N., Nkonya, E.M., Mairura, F.S., Chianu, J.N., and Akinnifesi, F.K. 2011. Biological nitrogen fixation and socioeconomic factors for legume production in sub-Saharan Africa: a review. Agron. Sust. Dev. 31, 139–154.
Compant, S.S., Clément, C., and Sessitsch, A. 2010. Plant growth-promoting bacteria in the rhizo- and endosphere of plants: their role, colonization, mechanisms involved and prospects for utilization. Soil Biol. Biochem. 42, 669–678.
Cotta, S.R., Mota, F.F., Tupinambá, G., Ishida, K., Rozental, S., Oliveira e Silva, D., Silva, A.J.R., Bizzo, H.R., Alviano, D.S., Alviano, C.S., and Seldin, L. 2011. Antimicrobial activity of Paenibacillus kribbensis POC 115 against the dermatophyte Trichophyton rubrum. World J. Microbiol. Biotechnol. 28, 953–962.
Dey, R., Pal, K.K., Bhatt, D.M., and Chauhan, S.M. 2004. Growth promotion and yield enhancement of peanut (Arachis hypogaea L.) by application of plant growth-promoting rhizobacteria. Microbiol. Res. 159, 371–394.
Dobbelaere, S., Vanderleyden, J., and Okon, Y. 2003. Plant growth-promoting effects of diazotrophs in the rhizosphere. Crit. Rev. Plant Sci. 22, 107–149.
Figueiredo, M.V.B., Sobral, J.K., Stamford, T.L.M., and Araújo, J.M. 2010. Bactérias promotoras do crescimento de plantas: estratégia para uma agricultura sustentável. pp. 387–414. In Figueiredo, M.V.B., Burity, H.A., Oliveira, J.P.O., Santos, C.E.R.S., and Stamford, N.P. (eds.), Biotecnologia aplicada à agricultura: textos de apoio e protocolos experimentais. Embrapa Agrobiologia, Brazil.
Gulden, R.H. and Vessey, J.K. 1998. Low concentrations of ammonium inhibit specific nodulation (nodule number g-1 root DW) in soybean (GlycIine max [L.] Merr.). Plant Soil 198, 127–136.
Hoagland, D. and Arnon, D.I. 1950. The water culture method for growing plants without soil. Agriculture Experimental Station Circular, California, USA.
Hungria, M. and Araújo, R.S. 1994. Manual de métodos empregados em estudos de microbiologia agrícola. Embrapa, Brazil.
Krapp, A., Berthomé, R., Orsel, M., Mercey-Boutet, S., Yu, A., Castaings, L., Elftieh, S., Major, H., Renou, J.P., and Daniel-Vedele, F. 2011. Arabidopsis roots and shoots show distinct temporal adaptation patterns toward nitrogen starvation. Plant Physiol. 157, 1255–1282.
Lima, A.S.T., Barreto, M.C.S., Araújo, J.M., Seldin, L., Burity, H.A. and Figueiredo, M.V.B. 2011. Sinergismo Bacillus, Brevibacillus e, ou, Paenibacillus na simbiose Bradyrhizobium-caupi. Rev. Bras. Cien. Solo 35, 713–721.
Lugtenberg, B. and Kamilova, F. 2009. Plant-growth-promoting rhizobacteria. Ann. Rev. Microbiol. 63, 541–556.
Maget-Dana, R. and Peypoux, F. 1994. Iturins, a special class of pore-forming lipopeptides: biological and physicochemical properties. Toxicology 87, 151–174.
Moreira, F.M.S. and Siqueira, J.O. 2006. Microbiologia e bioquímica do solo. UFLA, Lavras.
Reynolds, M. and Tuberosa, R. 2008. Translational research impacting on crop productivity in drought-prone environments. Curr. Opin. Plant Biol. 11, 171–179.
Robertson, G.P. and Vitousek, P.M. 2009. Nitrogen in agriculture: balancing the cost of an essential resource. Annu. Rev. Environ. Resour. 34, 97–125.
Silveira, J.A.G., Contado, J.L., Rodrigues, J.L.M., and Oliveira, J.T.A. 1998. Phosfoenolpyruvate carboxylase and glutamine synthetase activities in relation to nitrogen fixation in cowpea nodules. Rev. Br. Fis. Veg. 10, 19–23.
Spaepen, S., Vanderleyden, J., and Okon, Y. 2009. Plant growth-promoting actions of rhizobacteria. Adv. Bot. Res. 51, 283–320.
Velázquez, E., García-Fraile, P., Ramirez-Bahena, M.H., Rivas, R., and Martinez-Molina, E. 2010. Bacteria involved in nitrogen-fixing legume symbiosis: current taxonomic perspective. pp. 1–25. In Khan, M.S., Zaidi, A., and Musarrat, J. (ed.), Microbes for legume improvement. Springer, New York, N.Y., USA.
Vessey, K. and Buss, T.J. 2002. Bacillus cereus UW85 inoculation effects on growth, nodulation, and N accumulation in grain legumes: controlled-environment studies. Can. J. Plant Sci. 82, 282–290.
von der Weid, I., Paiva, E., Nóbrega, A., van Elsas, J.D., and Seldin, L. 2000. Diversity of Paenibacillus polymyxa strains isolated from the rhizosphere of maize planted in Cerrado soil. Res. Microbiol. 151, 369–381.
Yoon, J.H., Oh, H.M., Yoon, B.D., Kang, K., and Park, Y.H. 2003. Paenibacillus kribbensis sp. nov. and Paenibacillus terrae sp. nov., bioflocculants for ef_cient harvesting of algal cells. Int. J. Syst. Evol. Microbiol. 53, 295–301.
Zilli, J.E., Xavier, G.R., Moreira, F.M.S., Freitas, A.C.R., and Oliveira, L.A. 2009. Fixação biológica de nitrogênio. pp. 185–221. In Zilli, J.E., Vilarinho, A.A., and Alves, J.M.A. (eds.), A cultura do feijão-caupi na Amazônia Brasileira. Embrapa Roraima, Boa Vista.
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Rodrigues, A.C., Antunes, J.E.L., da Costa, A.F. et al. Interrelationship of Bradyrhizobium sp. and plant growth-promoting bacteria in cowpea: Survival and symbiotic performance. J Microbiol. 51, 49–55 (2013). https://doi.org/10.1007/s12275-013-2335-2
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DOI: https://doi.org/10.1007/s12275-013-2335-2